Validation Data Gallery
|Positive WB detected in||HEK-293T cells, mouse liver tissue, HeLa cells, Jurkat cells, C2C12 cells, NIH/3T3 cells, mouse skeletal muscle, rat skeletal muscle|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells, Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19096-1-AP is ACTN4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACTN4 fusion protein Ag13546|
|Full Name||actinin, alpha 4|
|Calculated molecular weight||105 kDa|
|Observed molecular weight||100-105 kDa|
|GenBank accession number||BC005033|
|Gene ID (NCBI)||81|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 100-105 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)
|Product Specific Protocols|
|WB protocol for ACTN4 antibody 19096-1-AP||Download protocol|
|IHC protocol for ACTN4 antibody 19096-1-AP||Download protocol|
|IF protocol for ACTN4 antibody 19096-1-AP||Download protocol|
|IP protocol for ACTN4 antibody 19096-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Front Cell Infect Microbiol
Transactivated Epidermal Growth Factor Receptor Recruitment of α-actinin-4 From F-actin Contributes to Invasion of Brain Microvascular Endothelial Cells by Meningitic Escherichia coli.
J Cell Biochem
Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.
Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.
J Extracell Vesicles
Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.
Cell Death Dis
NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.
J Thorac Oncol
Alpha-actinin 4 is Associated with Cancer Cell Motility and is a Potential Biomarker in Non-Small-Cell Lung Cancer.