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  • KD/KO Validated

ACTN4 Polyclonal antibody

ACTN4 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 19096-1-AP

Synonyms

ACTININ 4, actinin, alpha 4, ACTN4, Alpha actinin 4, F actin cross linking protein, FSGS, FSGS1, Non muscle alpha actinin 4



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells, NIH/3T3 cells, HepG2 cells, HT-1080 cells, mouse skeletal muscle tissue, C2C12 cell, mouse liver tissue, rat skeletal muscle tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, Hela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19096-1-AP is ACTN4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTN4 fusion protein Ag13546
Full Name actinin, alpha 4
Calculated molecular weight 105 kDa
Observed molecular weight 100-105 kDa
GenBank accession numberBC005033
Gene symbol ACTN4
Gene ID (NCBI) 81
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTN4 (alpha actinin 4) is a ubiquitous actin-binding protein that cross-links actin filaments into bundles to form filopodia, and has a crucial role in cytoskeletal organization. ACTN4 is colocalized with actin stress fibers and is dispersed in the cytoplasm and in the nucleus. Increased levels of ACTN4 have been associated with cancer metastasis, indicating its implication in cancer cell migration and metastasis. Defects in ACTN4 cause glomerular disease. This antibody recognizes 100-105 kDa of endogenous ACTN4 protein in various lysates. (22370640, 10700177)

Protocols

Product Specific Protocols
WB protocol for ACTN4 antibody 19096-1-APDownload protocol
IHC protocol for ACTN4 antibody 19096-1-APDownload protocol
IF protocol for ACTN4 antibody 19096-1-APDownload protocol
IP protocol for ACTN4 antibody 19096-1-APDownload protocol
FC protocol for ACTN4 antibody 19096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Front Cell Infect Microbiol

Transactivated Epidermal Growth Factor Receptor Recruitment of α-actinin-4 From F-actin Contributes to Invasion of Brain Microvascular Endothelial Cells by Meningitic Escherichia coli.

Authors - Jiyang Fu
mouseIF,WB

J Cell Biochem

Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.

Authors - Jun Zhong
humanWB,IHC,IF,CoIP

FASEB J

Trophoblastic proliferation and invasion regulated by ACTN4 is impaired in early onset preeclampsia.

Authors - Wei Peng
  • KD Validated
humanWB

J Extracell Vesicles

Hepatitis A virus structural protein pX interacts with ALIX and promotes the secretion of virions and foreign proteins through exosome-like vesicles.

Authors - Wang Jiang
humanWB

Cell Death Dis

NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.

Authors - Wenwen Chen
humanELISA

J Thorac Oncol

Alpha-actinin 4 is Associated with Cancer Cell Motility and is a Potential Biomarker in Non-Small-Cell Lung Cancer.

Authors - Ming-Chuan Wang