ADAM8 Antibody

ADAM8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat no : 23778-1-AP

Synonyms

ADAM metallopeptidase domain 8, ADAM8, ADAM8 protein, CD156, MS2



Tested Applications

Positive WB detected inBxPC-3 cells, mouse pancreas tissue
Positive IHC detected inhuman tonsillitis tissue, human pancreas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The 23778-1-AP immunogen is Fusion Protein ADAM8 protein expressed in E. coli.

Reactivity human, mouse
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM8 fusion protein Ag17262
Full Name ADAM metallopeptidase domain 8
Calculated molecular weight 824aa,89 kDa
Observed molecular weight 65 kDa
GenBank accession number BC115404
Gene symbol ADAM8
Gene ID (NCBI) 101
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAMs are a large family of transmembrane proteins that are involved in proteolysis, making them candidates for mediating the remodeling of the extracellular matrix(ECM). ADAM8, one of the members of the ADAM family, is overexpressed in various human tumors. It has been shown previously that hypoxia influences the expression of some ADAM proteins. The protein found to be expressed in two active forms(90 kDa and 60 kDa): potential shedding protein and remnant protein in the pancreatic tissues under chronic pancreatitis and pancreatic cancer(PMID:18566576). The full length protein has a signal peptide and four glycosylation sites. This antibody is specific to ADAM8.

Protocols

Product Specific Protocols
WB protocol for ADAM8 antibody 23778-1-APDownload protocol
IHC protocol for ADAM8 antibody 23778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Mol Med

ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.

Authors - Wei Li
mouseWB

Nat Med

Translation control of the immune checkpoint in cancer and its therapeutic targeting.

Authors - Yichen Xu
humanWB

Pathol Oncol Res

High ADAM8 expression is associated with poor prognosis in patients with hepatocellular carcinoma.

Authors - Yun Zhang