|Positive WB detected in||BxPC-3 cells, mouse pancreas tissue|
|Positive IHC detected in||human tonsillitis tissue, human pancreas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The 23778-1-AP immunogen is Fusion Protein ADAM8 protein expressed in E. coli.
|Cited Species||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ADAM8 fusion protein Ag17262|
|Full Name||ADAM metallopeptidase domain 8|
|Calculated molecular weight||824aa,89 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC115404|
|Gene ID (NCBI)||101|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20oC. Aliquoting is unnecessary for -20oC storage.|
ADAMs are a large family of transmembrane proteins that are involved in proteolysis, making them candidates for mediating the remodeling of the extracellular matrix(ECM). ADAM8, one of the members of the ADAM family, is overexpressed in various human tumors. It has been shown previously that hypoxia influences the expression of some ADAM proteins. The protein found to be expressed in two active forms(90 kDa and 60 kDa): potential shedding protein and remnant protein in the pancreatic tissues under chronic pancreatitis and pancreatic cancer(PMID:18566576). The full length protein has a signal peptide and four glycosylation sites. This antibody is specific to ADAM8.
Int J Mol Med
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Translation control of the immune checkpoint in cancer and its therapeutic targeting.
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High ADAM8 expression is associated with poor prognosis in patients with hepatocellular carcinoma.