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  • KD/KO Validated

ADAM8 Polyclonal antibody

ADAM8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 23778-1-AP


ADAM metallopeptidase domain 8, ADAM8, ADAM8 protein, CD156, MS2

Tested Applications

Positive WB detected inBxPC-3 cells, mouse pancreas tissue
Positive IHC detected inhuman tonsillitis tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23778-1-AP is ADAM8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM8 fusion protein Ag17262
Full Name ADAM metallopeptidase domain 8
Calculated Molecular Weight 824 aa, 89 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC115404
Gene Symbol ADAM8
Gene ID (NCBI) 101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADAMs are a large family of transmembrane proteins that are involved in proteolysis, making them candidates for mediating the remodeling of the extracellular matrix(ECM). ADAM8, one of the members of the ADAM family, is overexpressed in various human tumors. It has been shown previously that hypoxia influences the expression of some ADAM proteins. The protein found to be expressed in two active forms(90 kDa and 60 kDa): potential shedding protein and remnant protein in the pancreatic tissues under chronic pancreatitis and pancreatic cancer(PMID:18566576). The full length protein has a signal peptide and four glycosylation sites. This antibody is specific to ADAM8.


Product Specific Protocols
WB protocol for ADAM8 antibody 23778-1-APDownload protocol
IHC protocol for ADAM8 antibody 23778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell Neurosci

Matrix metalloprotease-mediated cleavage of neural glial-related cell adhesion molecules activates quiescent olfactory stem cells via EGFR.

Authors - Zhen-Huang Chen

Exp Anim

Suppression of ADAM8 attenuates angiotensin II-induced cardiac fibrosis and endothelial-mesenchymal transition via inhibiting TGF-β1/Smad2/Smad3 pathways.

Authors - Lixia Yao
  • KD Validated

J Healthc Eng

ADAM8 Activates NLRP3 Inflammasome to Promote Cerebral Ischemia-Reperfusion Injury.

Authors - Hongwei Lu

Pathol Oncol Res

High ADAM8 expression is associated with poor prognosis in patients with hepatocellular carcinoma.

Authors - Yun Zhang

Int J Mol Med

ADAM17 promotes lymph node metastasis in gastric cancer via activation of the Notch and Wnt signaling pathways.

Authors - Wei Li

Nat Med

Translation control of the immune checkpoint in cancer and its therapeutic targeting.

Authors - Yichen Xu