Tested Applications
| Positive WB detected in | A431 cells, HepG2 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human colon cancer tissue, human gliomas tissue, human stomach cancer tissue, mouse brain tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14330-1-AP targets ADAR1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5609 Product name: Recombinant human ADAR1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 877-1226 aa of BC038227 Sequence: DSEDMGVVVSLGTGNRCVKGDSLSLKGETVNDCHAEIISRRGFIRFLYSELMKYNSQTAKDSIFEPAKGGEKLQIKKTVSFHLYISTAPCGDGALFDKSCSDRAMESTESRHYPVFENPKQGKLRTKVENGEGTIPVESSDIVPTWDGIRLGERLRTMSCSDKILRWNVLGLQGALLTHFLQPIYLKSVTLGYLFSQGHLTRAICCRVTRDGSAFEDGLRHPFIVNHPKVGRVSIYDSKRQSGKTKETSVNWCLADGYDLEILDGTRGTVDGPRNELSRVSKKNIFLLFKKLCSFRYRRDLLRLSYGEAKKAARDYETAKNYFKKGLKDMGYGNWISKPQEEKNFYLCPV Predict reactive species |
| Full Name | adenosine deaminase, RNA-specific |
| Calculated Molecular Weight | 136 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC038227 |
| Gene Symbol | ADAR1 |
| Gene ID (NCBI) | 103 |
| RRID | AB_2273600 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55265 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ADAR1 antibody 14330-1-AP | Download protocol |
| IHC protocol for ADAR1 antibody 14330-1-AP | Download protocol |
| IP protocol for ADAR1 antibody 14330-1-AP | Download protocol |
| WB protocol for ADAR1 antibody 14330-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ADAR1 (p110) antibody (Cat# 14330-1-AP) has 34 citations across journals including Nature Cancer, Nature Communications, Redox Biology, Nucleic Acids Research, EMBO Journal, Cell Reports, and Journal of Biological Chemistry. Research fields span oncology (prostate, pancreatic, lung, colorectal cancers), RNA editing, neurobiology (depression, cognitive impairment), virology (SARS-CoV-2), immunology, toxicology, and rheumatology.
| Species | Application | Title |
|---|---|---|
Nat Commun Characterization of dsRNA binding proteins through solubility analysis identifies ZNF385A as a dsRNA homeostasis regulator
| ||
Nat Commun All-trans retinoic acid destabilizes ADAR1 protein through retinoylation-mediated USP7 dissociation and improves immunotherapy in pancreatic cancer. | ||
Redox Biol ADAR1 exacerbates ischemic brain injury via astrocyte-mediated neuron apoptosis | ||
Nucleic Acids Res Coronavirus S protein alters dsRNA accumulation and stress granule formation through regulation of ADAR1-p150 expression | ||
Genes Dis 8-Chloroadenosine suppresses hepatocellular carcinoma progression via ADAR1/PPARγ axis-mediated lipid metabolism.
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Reviews
What customers say
One reviewer (Emile A., Feb 2026) rated this antibody 5/5 stars after using it in Western Blot on U2OS cells at a 1:1000 dilution (Lot 00131460). They reported strong signal reduction upon siRNA-mediated ADAR1 knockdown, confirming high specificity and low background, and described the antibody as very reliable overall.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Emile (Verified Customer) (02-11-2026) | This antibody performed very well in Western blot. Upon siRNA-mediated knockdown of ADAR1, the signal was strongly reduced, confirming high specificity and low background. Overall, this antibody is very reliable.
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