ADAR1 Antibody

ADAR1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14330-1-AP

Synonyms

ADAR, ADAR1, adenosine deaminase, RNA, DRADA, DSH, DSRAD, G1P1, IFI 4, IFI4, K88dsRBP, p136



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inY79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14330-1-AP targets ADAR1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAR1 fusion protein Ag5609
Full Name adenosine deaminase, RNA-specific
Calculated molecular weight 136 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC038227
Gene symbol ADAR
Gene ID (NCBI) 103
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).

Protocols

Product Specific Protocols
WB protocol for ADAR1 antibody 14330-1-APDownload protocol
IHC protocol for ADAR1 antibody 14330-1-APDownload protocol
IF protocol for ADAR1 antibody 14330-1-APDownload protocol
IP protocol for ADAR1 antibody 14330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Increased AT2R expression is induced by AT1R autoantibody via two axes, Klf-5/IRF-1 and circErbB4/miR-29a-5p, to promote VSMC migration.

Authors - Yan Sun
mouseWB,IHC

PeerJ

PMID: 27602277

Authors - Wei Chen
mouseWB,IHC

Brain Behav

5-HT2CR antagonist/5-HT2CR inverse agonist recovered the increased isolation-induced aggressive behavior of BALB/c mice mediated by ADAR1 (p110) expression and Htr2c RNA editing.

Authors - Weizhi Yu
humanWB

World J Gastroenterol

LncRNA MEG3 acts a biomarker and regulates cell functions by targeting ADAR1 in colorectal cancer.

Authors - Wei Wang
mouseIHC

Toxicol Lett

Decrease in ADAR1 expression by exposure to cigarette smoke enhances susceptibility to oxidative stress.

Authors - Masashi Takizawa
humanIHC

Am J Cancer Res

The aberrant expression of ADAR1 promotes resistance to BET inhibitors in pancreatic cancer by stabilizing c-Myc.

Authors - Yan Sun