|Positive WB detected in||HeLa cells, HepG2 cells|
|Positive IP detected in||Y79 cells|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14330-1-AP targets ADAR1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ADAR1 fusion protein Ag5609|
|Full Name||adenosine deaminase, RNA-specific|
|Calculated molecular weight||136 kDa|
|Observed molecular weight||110 kDa|
|GenBank accession number||BC038227|
|Gene ID (NCBI)||103|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ADAR1 is also named as ADAR1, DSRAD, G1P1, IFI4. It convert selected adenosine residues into inosine in substrate RNAs containing a relatively short dsRNA region(PMID:15556947). The human ADAR1 gene specifies two size forms of RNA-specific adenosine deaminase, an IFN inducible ∼150 kDa protein and a constitutively expressed N-terminally truncated ∼110 kDa protein, encoded by transcripts with alternative exon 1 structures that initiate from different promoters(PMID:11111054). It has 5 isoforms produced by alternative promoter usage and alternative splicing. Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH).ADAR1 can form respective homodimers, and this association is essential for its enzymatic activities(PMID:17428802).
|Product Specific Protocols|
|WB protocol for ADAR1 antibody 14330-1-AP||Download protocol|
|IHC protocol for ADAR1 antibody 14330-1-AP||Download protocol|
|IF protocol for ADAR1 antibody 14330-1-AP||Download protocol|
|IP protocol for ADAR1 antibody 14330-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Cell Death Dis
Increased AT2R expression is induced by AT1R autoantibody via two axes, Klf-5/IRF-1 and circErbB4/miR-29a-5p, to promote VSMC migration.
5-HT2CR antagonist/5-HT2CR inverse agonist recovered the increased isolation-induced aggressive behavior of BALB/c mice mediated by ADAR1 (p110) expression and Htr2c RNA editing.
World J Gastroenterol
LncRNA MEG3 acts a biomarker and regulates cell functions by targeting ADAR1 in colorectal cancer.
Decrease in ADAR1 expression by exposure to cigarette smoke enhances susceptibility to oxidative stress.
Am J Cancer Res
The aberrant expression of ADAR1 promotes resistance to BET inhibitors in pancreatic cancer by stabilizing c-Myc.