Adiponectin Polyclonal antibody

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  WB analysis using 21613-1-AP

  Human plasma was subjected to SDS PAGE followed by western blot with 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 21613-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21613-1-AP (ADIPOQ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 21613-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 21613-1-AP (ADIPOQ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brown adipose using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brown adipose using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21613-1-AP (ADIPOQ antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 21613-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21613-1-AP (ADIPOQ antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of 3T3-L1 using 21613-1-AP

  Immunofluorescent analysis of (4% PFA) fixed 3T3-L1 cells using Adiponectin antibody (21613-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of NIH/3T3 using 21613-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Adiponectin antibody (21613-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Adiponectin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21613-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ADIPOQ, apM-1, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein

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Tested Applications

• Positive WB detected in: human plasma, NIH/3T3 cells, 3T3-L1 cells
• Positive IHC detected in: rat brown adipose tissue, human placenta tissue, human prostate cancer tissue, human skin tissue, mouse brown adipose tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: 3T3-L1 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Product Information

21613-1-AP targets Adiponectin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, sheep, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Adiponectin fusion protein Ag16304
• Full Name: adiponectin, C1Q and collagen domain containing
• Calculated Molecular Weight: 244 aa, 26 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC096308
• Gene Symbol: Adiponectin
• Gene ID (NCBI): 9370
• RRID: AB_2878891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15848
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Publications

Species	Application	Title
mouse	IF	Diabetes; PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ; Authors - Yi Zhong
human	WB	Int J Mol Sci; Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.; Authors - Weiyun Zhang
mouse	WB	Respir Res; Isthmin-1 attenuates allergic Asthma by stimulating adiponectin expression and alveolar macrophage efferocytosis in mice; Authors - Jong Huat Tee
mouse	WB	J Nutr Biochem; All-trans retinoic acid induces lipophagy through the activation of the AMPK-Beclin1 signaling pathway and reduces Rubicon expression in adipocytes; Authors - Yuki Mori
human	WB, IF	Sci China Life Sci; Compression loading of osteoclasts attenuated microRNA-146a-5p expression, which promotes angiogenesis by targeting adiponectin.; Authors - Yue Wang
mouse	WB	Dig Dis Sci; Adiponectin Alleviates Intestinal Fibrosis by Enhancing AMP-Activated Protein Kinase Phosphorylation.; Authors - Minghao Xie