Adiponectin Polyclonal antibody
Adiponectin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, ELISA
Cat no : 21613-1-AP
Validation Data Gallery
|Positive WB detected in||NIH/3T3 cells, 3T3-L1 cells|
|Positive IHC detected in||human liver tissue, human skin tissue, human placenta tissue, human prostate cancer tissue, rat brown adipose, mouse skeletal muscle tissue, mouse brown adipose tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21613-1-AP is Adiponectin Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Adiponectin fusion protein Ag16304|
|Full Name||adiponectin, C1Q and collagen domain containing|
|Calculated molecular weight||244 aa, 26 kDa|
|Observed molecular weight||29 kDa|
|GenBank accession number||BC096308|
|Gene ID (NCBI)||9370|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.
|Product Specific Protocols|
|WB protocol for Adiponectin antibody 21613-1-AP||Download protocol|
|IHC protocol for Adiponectin antibody 21613-1-AP||Download protocol|
|IF protocol for Adiponectin antibody 21613-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Sci China Life Sci
Compression loading of osteoclasts attenuated microRNA-146a-5p expression, which promotes angiogenesis by targeting adiponectin.
Dig Dis Sci
Adiponectin Alleviates Intestinal Fibrosis by Enhancing AMP-Activated Protein Kinase Phosphorylation.
Adiponectin protects obesity-related glomerulopathy by inhibiting ROS/NF-κB/NLRP3 inflammation pathway.
Cell Biol Int
IL-1α inhibits proliferation and adipogenic differentiation of human adipose-derived mesenchymal stem cells through NF-κB- and ERK1/2- mediated proinflammatory cytokines.
Int J Mol Sci
Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.
Activation of PPARγ Protects Obese Mice from Acute Lung Injury by Inhibiting Endoplasmic Reticulum Stress and Promoting Mitochondrial Biogenesis