Adrenomedullin Polyclonal antibody

Adrenomedullin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10778-1-AP

Synonyms

ADM, ProAM N terminal 20 peptide, Proadrenomedullin N-20 terminal peptide, Pro-adrenomedullin, PAMP

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Validation Data Gallery

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  WB analysis of human kidney using 10778-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10778-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10778-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10778-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 10778-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10778-1-AP (Adrenomedullin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10778-1-AP (Adrenomedullin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10778-1-AP (Adrenomedullin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10778-1-AP (Adrenomedullin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10778-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10778-1-AP (Adrenomedullin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 10778-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Adrenomedullin antibody (10778-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of A549 using 10778-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 10778-1-AP (Adrenomedullin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human kidney tissue, fetal human brain tissue, Raji cells, A549 cells, human placenta tissue
• Positive IHC detected in: human placenta tissue, human pancreas cancer tissue, human kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10778-1-AP targets Adrenomedullin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Adrenomedullin fusion protein Ag1197
• Full Name: adrenomedullin
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 6 kDa
• GenBank Accession Number: BC015961
• Gene Symbol: Adrenomedullin/ADM
• Gene ID (NCBI): 133
• RRID: AB_2242255
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP) are two small active hormones derived from the expression of a single gene (Adm) that is expressed throughout the GI tract, including the mucosal epithelium, glandular duct cells, neuroendocrine cells, and smooth muscle cells of the GI tract, between the oral cavity and the rectum (PMID:10782362, PMID:27345325). These two peptides coexist in GI cells, where they regulate many physiological functions including vasodilation, angiogenesis, anti-inflammation, organ protection, and tissue repair. AM suppresses inflammatory cytokine production in the intestinal mucosa, improves vascular and lymphatic function, mucosal epithelial repair, and intestinal barrier function in animal models with intestinal inflammation (PMID:27965594, PMID:29311984). Molecular mass species of 18, 14, and 6 kDa were identified in tumor cell lysates and presumably represent AM precursor, processed intermediates, and the authentic peptide, respectively. There is also a 22-kDa immunoreactive species in two cancer cell lines, H720 and MCF-7 (PMID: 8798536).

Publications

Species	Application	Title
human	IF	Cells; Differentiation Trajectory of Limbal Stem and Progenitor Cells under Normal Homeostasis and upon Corneal Wounding.; Authors - Zhenwei Song
human	IHC	Cell Mol Life Sci; APE1 controls DICER1 expression in NSCLC through miR-33a and miR-130b.; Authors - Giulia Antoniali
human	WB	Prostate; Prostate fibroblasts enhance androgen receptor splice variant 7 expression in prostate cancer cells; Authors - Takeshi Sasaki
human	WB	CNS Neurosci Ther; Identification and validation of a glycolysis-related taxonomy for improving outcomes in glioma; Authors - Tianshu Ying