ADRB2 Polyclonal antibody

ADRB2 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 13096-1-AP


ADRB2, ADRB2R, ADRBR, B2AR, BAR, Beta 2 adrenergic receptor, Beta 2 adrenoceptor, Beta 2 adrenoreceptor, BETA2AR

Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-3 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

13096-1-AP targets ADRB2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRB2 fusion protein Ag3715
Full Name adrenergic, beta-2-, receptor, surface
Calculated molecular weight46 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC012481
Gene symbol ADRB2
Gene ID (NCBI) 154
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2 adrenergic receptor (ADRB2), a member of the G protein-coupled receptor (GPCR) family, binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. ADRB2 is directly linked to one of its final effectors, the class C L-type Ca2+ channel, Cav1.2 (PMID: 11441182). This receptor-channel complex also contains a G protein, an adenylyl cyclase, cyclic adenosine monophosphate-dependent protein kinase (PKA), and a counteracting phosphatase, PP2A. Different polymorphic forms, point mutations, and/or downregulation of the gene of ADRB2 are associated with nocturnal asthma, obesity and type 2 diabetes. This antibody detects ADRB2 with an apparent molecular weight of 50-55 kDa as has been shown by some researches (PMID: 25348166; 12502786; 29232706; 23984976; 19584355).


Product Specific Protocols
WB protocol for ADRB2 antibody 13096-1-APDownload protocol
IHC protocol for ADRB2 antibody 13096-1-APDownload protocol
FC protocol for ADRB2 antibody 13096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Mol Sci

β-Adrenoceptor Activation in Breast MCF-10A Cells Induces a Pattern of Catecholamine Production Similar to that of Tumorigenic MCF-7 Cells.

Authors - Filipa Amaro

Sci Rep

SMN deficiency causes pain hypersensitivity in a mild SMA mouse model through enhancing excitability of nociceptive dorsal root ganglion neurons.

Authors - Ruobing Qu

J Otolaryngol Head Neck Surg

Propranolol as a novel adjunctive treatment for head and neck squamous cell carcinoma.

Authors - Nikolaus E Wolter

Open Med (Wars)

β2-Adrenergic receptor expression in subchondral bone of patients with varus knee osteoarthritis.

Authors - Xiaochun Yang

Oncol Rep

PMID: 27432558

Authors - Wei-Jun Wei

J Transl Med

Stress-induced epinephrine promotes epithelial-to-mesenchymal transition and stemness of CRC through the CEBPB/TRIM2/P53 axis.

Authors - Zili Zhou


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Elisa (Verified Customer) (04-14-2022)

It didn´t work. I used this antibody costained with a marker for microglia (Iba1) and a marker for neuron (neun). I tried two different protocols of immunofluorescence and one of them was with an antigen retrieval step. In both cases I didn´t have luck in obtaining a good result. You can see in the images that I attached.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: tissue, mouse spinal cord
ADRB2 Antibody Immunofluorescence validation (1:50 dilution) in tissue, mouse spinal cord (Cat no:13096-1-AP)

Tongbin (Verified Customer) (08-25-2020)

This antibody works beautifully in western blots.

  • Applications: Western Blot
  • Cell Tissue Type: Heart