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ADRB2 Polyclonal antibody
ADRB2 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, FC, ELISA
Cat no : 13096-1-AP
|Positive WB detected in||mouse heart tissue, rat heart tissue|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||PC-3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 12 publications below|
|IHC||See 3 publications below|
|IF||See 5 publications below|
13096-1-AP targets ADRB2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ADRB2 fusion protein Ag3715|
|Full Name||adrenergic, beta-2-, receptor, surface|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC012481|
|Gene ID (NCBI)||154|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Beta-2 adrenergic receptor (ADRB2), a member of the G protein-coupled receptor (GPCR) family, binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. ADRB2 is directly linked to one of its final effectors, the class C L-type Ca2+ channel, Cav1.2 (PMID: 11441182). This receptor-channel complex also contains a G protein, an adenylyl cyclase, cyclic adenosine monophosphate-dependent protein kinase (PKA), and a counteracting phosphatase, PP2A. Different polymorphic forms, point mutations, and/or downregulation of the gene of ADRB2 are associated with nocturnal asthma, obesity and type 2 diabetes. This antibody detects ADRB2 with an apparent molecular weight of 50-55 kDa as has been shown by some researches (PMID: 25348166; 12502786; 29232706; 23984976; 19584355).
|Product Specific Protocols|
|WB protocol for ADRB2 antibody 13096-1-AP||Download protocol|
|IHC protocol for ADRB2 antibody 13096-1-AP||Download protocol|
|FC protocol for ADRB2 antibody 13096-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Proc Natl Acad Sci U S A
Astrocytic β2-adrenergic receptors mediate hippocampal long-term memory consolidation.
Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.
Mol Ther Nucleic Acids
Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.
Interplay between sympathetic nervous system and inflammation in aseptic loosening of hip joint replacement.
β2-adrenoreceptor agonist ameliorates mechanical allodynia in paclitaxel-induced neuropathic pain via induction of mitochondrial biogenesis.
Drug Des Devel Ther
10-Gingerol Enhances the Effect of Taxol in Triple-Negative Breast Cancer via Targeting ADRB2 Signaling
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Elisa (Verified Customer) (04-14-2022)
It didn´t work. I used this antibody costained with a marker for microglia (Iba1) and a marker for neuron (neun). I tried two different protocols of immunofluorescence and one of them was with an antigen retrieval step. In both cases I didn´t have luck in obtaining a good result. You can see in the images that I attached.
Tongbin (Verified Customer) (08-25-2020)
This antibody works beautifully in western blots.