ADRB2 Antibody

ADRB2 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13096-1-AP

Synonyms

ADRB2, ADRB2R, ADRBR, B2AR, BAR, Beta 2 adrenergic receptor, Beta 2 adrenoceptor, Beta 2 adrenoreceptor, BETA2AR



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

13096-1-AP targets ADRB2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRB2 fusion protein Ag3715
Full Name adrenergic, beta-2-, receptor, surface
Calculated molecular weight 413aa,46.6 kDa
Observed molecular weight 50-55 kDa
GenBank accession number BC012481
Gene symbol ADRB2
Gene ID (NCBI) 154
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beta-2 adrenergic receptor (ADRB2), a member of the G protein-coupled receptor (GPCR) family, binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. ADRB2 is directly linked to one of its final effectors, the class C L-type Ca2+ channel, Cav1.2 (PMID: 11441182). This receptor-channel complex also contains a G protein, an adenylyl cyclase, cyclic adenosine monophosphate- dependent protein kinase (PKA), and a counteracting phosphatase, PP2A. Different polymorphic forms, point mutations, and/or downregulation of the gene of ADRB2 are associated with nocturnal asthma, obesity and type 2 diabetes. This antibody detects ADRB2 with an apparent molecular weight of 50-55 kDa as has been shown by some researches (PMID: 25348166; 12502786; 29232706; 23984976; 19584355).

Protocols

Product Specific Protocols
WB protocol for ADRB2 antibody 13096-1-APDownload protocol
IHC protocol for ADRB2 antibody 13096-1-APDownload protocol
FC protocol for ADRB2 antibody 13096-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Rep

PMID: 27432558

Authors - Wei-Jun Wei
humanIF

Sci Rep

Interplay between sympathetic nervous system and inflammation in aseptic loosening of hip joint replacement.

Authors - M Ribeiro-da-Silva
ratWB,IHC

Proc Natl Acad Sci U S A

Astrocytic β2-adrenergic receptors mediate hippocampal long-term memory consolidation.

Authors - Virginia Gao