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ADRM1 Polyclonal antibody

ADRM1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11468-1-AP

Synonyms

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, PC-3 cells, Raji cells, NIH/3T3 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11468-1-AP targets ADRM1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRM1 fusion protein Ag1997
Full Name adhesion regulating molecule 1
Calculated Molecular Weight 407 aa, 42 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC017245
Gene Symbol ADRM1
Gene ID (NCBI) 11047
RRIDAB_2273873
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.

Protocols

Product Specific Protocols
WB protocol for ADRM1 antibody 11468-1-APDownload protocol
IHC protocol for ADRM1 antibody 11468-1-APDownload protocol
IF protocol for ADRM1 antibody 11468-1-APDownload protocol
IP protocol for ADRM1 antibody 11468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

An Interaction Landscape of Ubiquitin Signaling.

Authors - Xiaofei Zhang
mouseIHC

Proc Natl Acad Sci U S A

Dendritic spinopathy in transgenic mice expressing ALS/dementia-linked mutant UBQLN2.

Authors - George H Gorrie
WB

Cell Rep

SUMOylation of Psmd1 Controls Adrm1 Interaction with the Proteasome.

Authors - Hyunju Ryu
humanWB

Oncogene

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Authors - Sparks A A
humanIHC

Genes Chromosomes Cancer

Comprehensive analysis of 20q13 genes in ovarian cancer identifies ADRM1 as amplification target.

Authors - Fejzo Marlena S MS
mouseIF

Biochem Biophys Res Commun

Disturbance of proteasomal and autophagic protein degradation pathways by amyotrophic lateral sclerosis-linked mutations in ubiquilin 2.

Authors - Mayuko Osaka