Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse testis tissue, mouse ovary tissue, HeLa cells|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11468-1-AP targets ADRM1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ADRM1 fusion protein Ag1997|
|Full Name||adhesion regulating molecule 1|
|Calculated molecular weight||407 aa, 42 kDa|
|Observed molecular weight||46 kDa|
|GenBank accession number||BC017245|
|Gene ID (NCBI)||11047|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.
|Product Specific Protocols|
|WB protocol for ADRM1 antibody 11468-1-AP||Download protocol|
|IHC protocol for ADRM1 antibody 11468-1-AP||Download protocol|
|IF protocol for ADRM1 antibody 11468-1-AP||Download protocol|
|IP protocol for ADRM1 antibody 11468-1-AP||Download protocol|
|FC protocol for ADRM1 antibody 11468-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A possible connection between adhesion regulating molecule 1 overexpression and nuclear factor kappa B activity in hepatocarcinogenesis.
Biochem Biophys Res Commun
Disturbance of proteasomal and autophagic protein degradation pathways by amyotrophic lateral sclerosis-linked mutations in ubiquilin 2.
Genes Chromosomes Cancer
Comprehensive analysis of 20q13 genes in ovarian cancer identifies ADRM1 as amplification target.
The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.
SUMOylation of Psmd1 Controls Adrm1 Interaction with the Proteasome.
Proc Natl Acad Sci U S A
Dendritic spinopathy in transgenic mice expressing ALS/dementia-linked mutant UBQLN2.