ADRM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ADRM1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 11468-1-AP

Synonyms

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog

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Validation Data Gallery

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  WB analysis of HeLa using 11468-1-AP

  WB result of ADRM1 antibody (11468-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADRM1 transfected HeLa cells.

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  WB analysis using 11468-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11468-1-AP (ADRM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11468-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11468-1-AP (ADRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11468-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11468-1-AP (ADRM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 11468-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11468-1-AP (ADRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11468-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11468-1-AP (ADRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 11468-1-AP

  IP Result of anti-ADRM1 (IP:11468-1-AP, 3ug; Detection:11468-1-AP 1:500) with mouse testis tissue lysate 8000ug.

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  IHC staining of human colon cancer using 11468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11468-1-AP (ADRM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11468-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11468-1-AP (ADRM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 11468-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ADRM1 antibody 11468-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of MCF-7 using 11468-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug ADRM1 antibody (11468-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse testis tissue, mouse ovary tissue, HeLa cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

11468-1-AP targets ADRM1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ADRM1 fusion protein Ag1997
• Full Name: adhesion regulating molecule 1
• Calculated molecular weight: 407 aa, 42 kDa
• Observed molecular weight: 46 kDa
• GenBank accession number: BC017245
• Gene symbol: ADRM1
• Gene ID (NCBI): 11047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.

Publications

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
mouse	IHC	Proc Natl Acad Sci U S A; Dendritic spinopathy in transgenic mice expressing ALS/dementia-linked mutant UBQLN2.; Authors - George H Gorrie
	WB	Cell Rep; SUMOylation of Psmd1 Controls Adrm1 Interaction with the Proteasome.; Authors - Hyunju Ryu
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A
human	IHC	Genes Chromosomes Cancer; Comprehensive analysis of 20q13 genes in ovarian cancer identifies ADRM1 as amplification target.; Authors - Fejzo Marlena S MS
mouse	IF	Biochem Biophys Res Commun; Disturbance of proteasomal and autophagic protein degradation pathways by amyotrophic lateral sclerosis-linked mutations in ubiquilin 2.; Authors - Mayuko Osaka

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