Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, PC-3 cells, Raji cells, NIH/3T3 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11468-1-AP targets ADRM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1997 Product name: Recombinant human ADRM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-407 aa of BC017245 Sequence: MTTSGALFPSLVPGSRGASNKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVEDDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNEYLNNPPMPGALGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPGSSSSSSSRSQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNVPAGPAGGQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD Predict reactive species |
| Full Name | adhesion regulating molecule 1 |
| Calculated Molecular Weight | 407 aa, 42 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC017245 |
| Gene Symbol | ADRM1 |
| Gene ID (NCBI) | 11047 |
| RRID | AB_2273873 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16186 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ADRM1 antibody 11468-1-AP | Download protocol |
| IHC protocol for ADRM1 antibody 11468-1-AP | Download protocol |
| IP protocol for ADRM1 antibody 11468-1-AP | Download protocol |
| WB protocol for ADRM1 antibody 11468-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ADRM1 Polyclonal antibody (Cat# 11468-1-AP) has 11 citations published in journals including Mol Cell, PNAS, Oncogene, Cell Reports, Experimental Hematology Oncology, International Journal of Biological Macromolecules, Scientific Reports, Oncology Reports, Biochemical and Biophysical Research Communications, and Genes, Chromosomes & Cancer. Research fields span ubiquitin-proteasome signaling, neurodegeneration (ALS), and oncology (lung, bladder, kidney, ovarian, and liver cancers), as well as cartilage biology.
| Species | Application | Title |
|---|---|---|
Exp Hematol Oncol Downregulation of pro-surfactant protein B contributes to the recurrence of early-stage non-small cell lung cancer by activating PGK1-mediated Akt signaling
| ||
Proc Natl Acad Sci U S A Dendritic spinopathy in transgenic mice expressing ALS/dementia-linked mutant UBQLN2. | ||
Oncogene The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a. | ||
Int J Biol Macromol ADRM1/RPN13 attenuates cartilage extracellular matrix degradation via enhancing UCH37-mediated ALK5 deubiquitination
| ||
