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  • KD/KO Validated

ADRM1 Polyclonal antibody

ADRM1 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 11468-1-AP

Synonyms

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog



Tested Applications

Positive WB detected inHEK-293 cells, mouse testis tissue, mouse ovary tissue, HeLa cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11468-1-AP targets ADRM1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRM1 fusion protein Ag1997
Full Name adhesion regulating molecule 1
Calculated molecular weight 407 aa, 42 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC017245
Gene symbol ADRM1
Gene ID (NCBI) 11047
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.

Protocols

Product Specific Protocols
WB protocol for ADRM1 antibody 11468-1-APDownload protocol
IHC protocol for ADRM1 antibody 11468-1-APDownload protocol
IF protocol for ADRM1 antibody 11468-1-APDownload protocol
IP protocol for ADRM1 antibody 11468-1-APDownload protocol
FC protocol for ADRM1 antibody 11468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncol Rep

A possible connection between adhesion regulating molecule 1 overexpression and nuclear factor kappa B activity in hepatocarcinogenesis.

Authors - Yang Xin X
  • KD Validated
mouseIF

Biochem Biophys Res Commun

Disturbance of proteasomal and autophagic protein degradation pathways by amyotrophic lateral sclerosis-linked mutations in ubiquilin 2.

Authors - Mayuko Osaka
humanIHC

Genes Chromosomes Cancer

Comprehensive analysis of 20q13 genes in ovarian cancer identifies ADRM1 as amplification target.

Authors - Fejzo Marlena S MS
humanWB

Oncogene

The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.

Authors - Sparks A A
WB

Cell Rep

SUMOylation of Psmd1 Controls Adrm1 Interaction with the Proteasome.

Authors - Hyunju Ryu
mouseIHC

Proc Natl Acad Sci U S A

Dendritic spinopathy in transgenic mice expressing ALS/dementia-linked mutant UBQLN2.

Authors - George H Gorrie