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AFF4 Polyclonal antibody
AFF4 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 14662-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue |
Positive IP detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 15 publications below |
IHC | See 1 publications below |
IP | See 3 publications below |
Product Information
14662-1-AP targets AFF4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AFF4 fusion protein Ag6336 |
Full Name | AF4/FMR2 family, member 4 |
Calculated Molecular Weight | 127 kDa |
Observed Molecular Weight | 140-150 kDa |
GenBank Accession Number | BC063007 |
Gene Symbol | AFF4 |
Gene ID (NCBI) | 27125 |
RRID | AB_2242609 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].
Protocols
Product Specific Protocols | |
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WB protocol for AFF4 antibody 14662-1-AP | Download protocol |
IP protocol for AFF4 antibody 14662-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy. | ||
Nat Commun Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription | ||
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
Genes Dev Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression. | ||
Nucleic Acids Res Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.
| ||
Cell Discov Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription |