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  • KD/KO Validated

AFF4 Polyclonal antibody

AFF4 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14662-1-AP

Synonyms

AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31



Tested Applications

Positive WB detected inL02 cells, HeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14662-1-AP targets AFF4 in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFF4 fusion protein Ag6336
Full Name AF4/FMR2 family, member 4
Calculated molecular weight 127 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC063007
Gene symbol AFF4
Gene ID (NCBI) 27125
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].

Protocols

Product Specific Protocols
WB protocol for AFF4 antibody 14662-1-APDownload protocol
IF protocol for AFF4 antibody 14662-1-APDownload protocol
IP protocol for AFF4 antibody 14662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Discov

Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription.

Authors - Dan Tang
humanWB

Sci Rep

A chalcone derivative reactivates latent HIV-1 transcription through activating P-TEFb and promoting Tat-SEC interaction on viral promoter.

Authors - Jun Wu
humanWB

Nucleic Acids Res

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Authors - Xiaodong Lu
  • KD Validated
humanWB

Cell

Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy.

Authors - Kaiwei Liang