AFF4 Polyclonal antibody

• Featured Product
• KD/KO Validated

AFF4 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14662-1-AP

Synonyms

AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31

---

Validation Data Gallery

• 

  WB analysis using 14662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14662-1-AP (AFF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14662-1-AP

  IP result of anti-AFF4(IP:14662-1-AP, 4ug; Detection:14662-1-AP 1:20000) with HeLa cells lysate 1880 ug.

• 

  IF Staining of L02 using 14662-1-AP

  Immunofluorescent analysis of (4% PFA) fixed L02 cells using 14662-1-AP (AFF4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IF detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below

Product Information

14662-1-AP targets AFF4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AFF4 fusion protein Ag6336
• Full Name: AF4/FMR2 family, member 4
• Calculated molecular weight: 127 kDa
• Observed molecular weight: 140-150 kDa
• GenBank accession number: BC063007
• Gene symbol: AFF4
• Gene ID (NCBI): 27125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].

Publications

Species	Application	Title
human	WB	Cell; Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy.; Authors - Kaiwei Liang
human	WB	Genes Dev; Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression.; Authors - Bin Zheng
human	WB	Nucleic Acids Res; Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.; Authors - Xiaodong Lu ; KD Validated
human	WB	Cell Discov; Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription; Authors - Dan Tang
human	WB	Sci Rep; A chalcone derivative reactivates latent HIV-1 transcription through activating P-TEFb and promoting Tat-SEC interaction on viral promoter.; Authors - Jun Wu
human	WB	Cell Discov; Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription.; Authors - Dan Tang