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AFF4 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 14662-1-AP

Featured Product KD/KO validated

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Con: 22 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

AFF4 Antibody WB L02 cells 14662-1-AP

L02 cells were subjected to SDS PAGE followed by western blot with 14662-1-AP(AFF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

AFF4 Antibody IP HepG2 cells 14662-1-AP

IP Result of anti-AFF4 (IP:14662-1-AP, 4ug; Detection:14662-1-AP 1:300) with HepG2 cells lysate 3200ug.

AFF4 Antibody IF L02 cells 14662-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed L02 cells using 14662-1-AP(AFF4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat
Positive WB detected in:
L02 cells, HeLa cells, HepG2 cells
Positive IP detected in:
HepG2 cells
Positive IF detected in:
L02 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AFF4 fusion protein Ag6336
Full name:
AF4/FMR2 family, member 4
Calculated molecular weight:
127 kDa
Observed molecular weight:
140 kDa
GenBank accession number:
BC063007
Gene ID (NCBI):
27125
Gene symbol
AFF4
Synonyms
AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31

Background

Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].


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