- Featured Product
- KD/KO Validated
AFF4 Polyclonal antibody
AFF4 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
WB, IP, IF, ELISA
Cat no : 14662-1-AP
|Positive WB detected in||HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||L02 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14662-1-AP targets AFF4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AFF4 fusion protein Ag6336|
|Full Name||AF4/FMR2 family, member 4|
|Calculated molecular weight||127 kDa|
|Observed molecular weight||140-150 kDa|
|GenBank accession number||BC063007|
|Gene ID (NCBI)||27125|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].
|Product Specific Protocols|
|WB protocol for AFF4 antibody 14662-1-AP||Download protocol|
|IF protocol for AFF4 antibody 14662-1-AP||Download protocol|
|IP protocol for AFF4 antibody 14662-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy.
Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression.
Nucleic Acids Res
Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.
Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription
A chalcone derivative reactivates latent HIV-1 transcription through activating P-TEFb and promoting Tat-SEC interaction on viral promoter.
Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription.