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  • KD/KO Validated

AFF4 Polyclonal antibody

AFF4 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14662-1-AP

Synonyms

AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14662-1-AP targets AFF4 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFF4 fusion protein Ag6336
Full Name AF4/FMR2 family, member 4
Calculated molecular weight 127 kDa
Observed molecular weight 140-150 kDa
GenBank accession numberBC063007
Gene symbol AFF4
Gene ID (NCBI) 27125
RRIDAB_2242609
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].

Protocols

Product Specific Protocols
WB protocol for AFF4 antibody 14662-1-APDownload protocol
IP protocol for AFF4 antibody 14662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy.

Authors - Kaiwei Liang
mouseWB

Nat Commun

Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription

Authors - Mei Sheng Lau
human,mouseWB

Mol Cell

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Authors - Yuanjun Li
humanWB

Genes Dev

Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression.

Authors - Bin Zheng
humanWB

Nucleic Acids Res

Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.

Authors - Xiaodong Lu
  • KD Validated
humanWB

Cell Discov

Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription

Authors - Dan Tang