AFF4 Polyclonal antibody

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  WB analysis using 14662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14662-1-AP (AFF4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14662-1-AP

  IP result of anti-AFF4 (IP:14662-1-AP, 4ug; Detection:14662-1-AP 1:20000) with HeLa cells lysate 1880 ug.

• Featured Product
• KD/KO Validated

AFF4 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14662-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ELISA

Synonyms

AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

14662-1-AP targets AFF4 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AFF4 fusion protein Ag6336
• Full Name: AF4/FMR2 family, member 4
• Calculated Molecular Weight: 127 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC063007
• Gene Symbol: AFF4
• Gene ID (NCBI): 27125
• RRID: AB_2242609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHB7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcriptional controled by RNA polymerase II (Pol II) is a multi-step process requiring the concerted action of multiple factors and contacts with the DNA template for the proper synthesis of nascent RNA [PMID:12676794]. ELL1 was demonstrated to be a Pol II elongation factor capable of enhancing the catalytic rate of transcription elongation by reducing transient pausing by the enzyme [PMID:8596958]. AFF4 was initially identified as a fusion partner of the MLL (mixed-lineage leukemia) gene participated in infant acute lymphoblastic leukemia, and a component of the ELL/p-TEFb elongation complex. AFF4 interacts with positive transcription elongation factor-b while also repressing Tat transactivation of HIV-1 [PMID:17389929].

Publications

Species	Application	Title
human	WB	Cell; Targeting Processive Transcription Elongation via SEC Disruption for MYC-Induced Cancer Therapy.; Authors - Kaiwei Liang
mouse	WB	Nat Commun; Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription; Authors - Mei Sheng Lau
human,mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	WB	Genes Dev; Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression.; Authors - Bin Zheng
human	WB	Nucleic Acids Res; Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.; Authors - Xiaodong Lu ; KD Validated
human	WB	Cell Discov; Structural and functional insight into the effect of AFF4 dimerization on activation of HIV-1 proviral transcription; Authors - Dan Tang