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- KD/KO Validated
AGGF1 Polyclonal antibody
AGGF1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11889-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, HEK-293 cells, HeLa cells, rat liver tissue |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 10 publications below |
| IHC | See 5 publications below |
| IF | See 2 publications below |
Product Information
11889-1-AP targets AGGF1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rhesus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AGGF1 fusion protein Ag2497 |
| Full Name | angiogenic factor with G patch and FHA domains 1 |
| Calculated molecular weight | 714 aa, 81 kDa |
| Observed molecular weight | 84-100 kDa |
| GenBank accession number | BC029382 |
| Gene symbol | AGGF1 |
| Gene ID (NCBI) | 55109 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis. Catalog#11889-1-AP was validated by KO and KD methods in scientists' studies (PMID: 28153879; 27513923), and the observed MW is 84 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AGGF1 antibody 11889-1-AP | Download protocol |
| IHC protocol for AGGF1 antibody 11889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Int Knockdown of AGGF1 inhibits the invasion and migration of gastric cancer via epithelial-mesenchymal transition through Wnt/β-catenin pathway.
| ||
Biochim Biophys Acta Role of microRNA-27a in down-regulation of angiogenic factor AGGF1 under hypoxia associated with high-grade bladder urothelial carcinoma. | ||
Clin Oral Investig AGGF1 inhibits the expression of inflammatory mediators and promotes angiogenesis in dental pulp cells. | ||
J Biochem Mol Toxicol Angiogenic factor with G patch and FHA domains 1 protects retinal vascular endothelial cells under hyperoxia by inhibiting autophagy. | ||
Biochim Biophys Acta Angiogenic factor with G patch and FHA domains 1 (Aggf1) regulates liver fibrosis by modulating TGF-β signaling.
| ||
PLoS One Angiogenic factor AGGF1 promotes therapeutic angiogenesis in a mouse limb ischemia model. |
