AGGF1 Polyclonal antibody

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  WB analysis of HeLa using 11889-1-AP

  WB result of AGGF1 antibody (11889-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGGF1 transfected HeLa cells.

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  WB analysis using 11889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11889-1-AP (AGGF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

AGGF1 Polyclonal Antibody for WB, ELISA

Cat No. 11889-1-AP

Publications(25)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

HUS84971, HSU84971, GPATCH7, GPATC7, G patch domain-containing protein 7

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Tested Applications

• Positive WB detected in: HUVEC cells, HeLa cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 21 publications below
• IHC: See 9 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

11889-1-AP targets AGGF1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish, macaque, rhesus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AGGF1 fusion protein Ag2497
• Full Name: angiogenic factor with G patch and FHA domains 1
• Calculated Molecular Weight: 714 aa, 81 kDa
• Observed Molecular Weight: 84-100 kDa
• GenBank Accession Number: BC029382
• Gene Symbol: AGGF1
• Gene ID (NCBI): 55109
• RRID: AB_2305338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N302
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis.

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.; Authors - Yufeng Yao
mouse	WB	Mol Ther; The 3' Untranslated Region Protects the Heart from Angiotensin II-Induced Cardiac Dysfunction via AGGF1 Expression.; Authors - Lexi Ding
mouse	WB	PLoS Biol; Angiogenic Factor AGGF1 Activates Autophagy with an Essential Role in Therapeutic Angiogenesis for Heart Disease.; Authors - Qiulun Lu ; KO Validated
rat	WB,IF	Arterioscler. Thromb. Vasc. Biol.; Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury.; Authors - Bisheng Zhou ; KD Validated
human	WB	Mol Ther Nucleic Acids; circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways.; Authors - Qianru He
		FASEB J; Angiotensin II increases angiogenesis by NF-κB-mediated transcriptional activation of angiogenic factor AGGF1.; Authors - Wenxia Si