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  • KD/KO Validated

AGGF1 Antibody

AGGF1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11889-1-AP

Synonyms

AGGF1, Angiogenic factor VG5Q, GPATC7, GPATCH7, HSU84971, HUS84971, hVG5Q, VG5Q



Tested Applications

Positive WB detected inmouse liver tissue, HEK-293 cells, HeLa cells, rat liver tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11889-1-AP targets AGGF1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, rhesus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGGF1 fusion protein Ag2497
Full Name angiogenic factor with G patch and FHA domains 1
Calculated molecular weight 714 aa, 81 kDa
Observed molecular weight 84-100 kDa
GenBank accession numberBC029382
Gene symbol AGGF1
Gene ID (NCBI) 55109
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis. Catalog#11889-1-AP was validated by KO and KD methods in scientists' studies (PMID: 28153879; 27513923), and the observed MW is 84 kDa.

Protocols

Product Specific Protocols
WB protocol for AGGF1 antibody 11889-1-APDownload protocol
IHC protocol for AGGF1 antibody 11889-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell Int

Knockdown of AGGF1 inhibits the invasion and migration of gastric cancer via epithelial-mesenchymal transition through Wnt/β-catenin pathway.

Authors - Han-Hui Yao
  • KD Validated
humanWB,IHC

Biochim Biophys Acta

Role of microRNA-27a in down-regulation of angiogenic factor AGGF1 under hypoxia associated with high-grade bladder urothelial carcinoma.

Authors - Yan Xu
humanIHC

Clin Oral Investig

AGGF1 inhibits the expression of inflammatory mediators and promotes angiogenesis in dental pulp cells.

Authors - Song Shen
rhesusWB,IF

J Biochem Mol Toxicol

Angiogenic factor with G patch and FHA domains 1 protects retinal vascular endothelial cells under hyperoxia by inhibiting autophagy.

Authors - Guomin Yao
mouseWB

Biochim Biophys Acta

Angiogenic factor with G patch and FHA domains 1 (Aggf1) regulates liver fibrosis by modulating TGF-β signaling.

Authors - Bisheng Zhou
  • KD Validated
mouseWB,IHC

PLoS One

Angiogenic factor AGGF1 promotes therapeutic angiogenesis in a mouse limb ischemia model.

Authors - Lu Qiulun Q