AGGF1 Polyclonal antibody

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• KD/KO Validated

AGGF1 Polyclonal Antibody for WB, ELISA

Cat No. 11889-1-AP Copied!

Publications (28)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

HUS84971, HSU84971, GPATCH7, GPATC7, G patch domain-containing protein 7

• 

  WB analysis using 11889-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11889-1-AP (AGGF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 11889-1-AP

  WB result of AGGF1 antibody (11889-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGGF1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HUVEC cells, HeLa cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11889-1-AP targets AGGF1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish, macaque, rhesus
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2497

  Product name: Recombinant human AGGF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 4-270 aa of BC029382

  Sequence: DMEHTLRIPEVGVSKFHAEIYFDHDLQSYVPVDQGSQNGTIVNGKQILQPKTKCDPYVLEHGDEVKIGETVLSFHIHPGSDTCDGCEPGQVRAHLRLDKKDESFVGPTLSKEEKELERRKELKKIRVKYGLQNTEYEDEKTLKNPKYKDRAGKRREQVGSEGTFQRDDAPASVHSEITDSNKGRKMLEKMGWKKGEGLGKDGGGMKTPIQLQLRRTHAGLGTGKPSSFEDVHLLQNKNKKNWDKARERFTENFPETKPQKDDPGTMP

• Full Name: angiogenic factor with G patch and FHA domains 1
• Calculated Molecular Weight: 714 aa, 81 kDa
• Observed Molecular Weight: 84-100 kDa
• GenBank Accession Number: BC029382
• Gene Symbol: AGGF1
• Gene ID (NCBI): 55109
• RRID: AB_2305338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N302
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis.

Publications

What published studies show

The AGGF1 antibody (Cat# 11889-1-AP) has 28 citations across journals including Nature Communications, Molecular Therapy, FASEB Journal, Arterioscler Thromb Vasc Biol, PLoS Biology, J Biol Chem, Biochim Biophys Acta, and Cancer Cell Int. Research fields span angiogenesis, cardiovascular disease, liver fibrosis, retinal vascular development, cancer (gastric, glioma, bladder), neuroprotection, placenta accreta, and diabetic kidney disease.

Automatically generated from the 28 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Nat Commun; A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure.; Authors - Yufeng Yao
human,mouse	WB	Nat Commun; Endothelial AGGF1 promotes retinal angiogenesis by coordinating TNFSF12/FN14 signalling; Authors - Ying Cheng ; KD Validated
	WB,IHC	Nat Commun; AGGF1 therapy inhibits thoracic aortic aneurysms by enhancing integrin α7-mediated inhibition of TGF-β1 maturation and ERK1/2 signaling; Authors - Xingwen Da
mouse	WB	Mol Ther; The 3' Untranslated Region Protects the Heart from Angiotensin II-Induced Cardiac Dysfunction via AGGF1 Expression.; Authors - Lexi Ding
rat	WB,IF	Arterioscler. Thromb. Vasc. Biol.; Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury.; Authors - Bisheng Zhou ; KD Validated
human,zebrafish	WB	Arterioscler Thromb Vasc Biol; Aggf1 Specifies Hemangioblasts at the Top of Regulatory Hierarchy via Npas4l and mTOR-S6K-Emp2-ERK Signaling; Authors - Zhongcheng Yang ; KO Validated