AID Polyclonal antibody

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  WB analysis using 15642-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15642-1-AP (AID antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of human tonsillitis using 15642-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using AID antibody (15642-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AID Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 15642-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

AICDA, Activation-induced cytidine deaminase, ARP2, CDA2, Cytidine aminohydrolase

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Tested Applications

• Positive WB detected in: MG-63 cells, Ramos cells, THP-1 cells, U2OS cells, NIH/3T3 cells
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15642-1-AP targets AID in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8136

  Product name: Recombinant human AID protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-198 aa of BC006296

  Sequence: MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLDFGYLRNKNGCHVELLFLRYISDWDLDPGRCYRVTWFTSWSPCYDCARHVADFLRGNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFVENHERTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL

• Full Name: activation-induced cytidine deaminase
• Calculated Molecular Weight: 198 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC006296
• Gene Symbol: AIDA/AID
• Gene ID (NCBI): 57379
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9GZX7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AID is essential to all three genetic alterations required for antigen-specific immunoglobulin: class switch recombination (CSR), somatic bypermutation (SHM), and gene conversion. Expression of AID is normally restricted to B cells in the GC, where SHM and CSR occur. It is likely these represent AID monomer and homodimer that western blot detected two bands in purified AID from nuclear extracts, one at 52 kDa and the other at 26 kDa. (PMID:16280388)