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  • KD/KO Validated

AIF Polyclonal antibody

AIF Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17984-1-AP

Synonyms

AIF, AIFM1, PDCD8



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, A549 cells, rat kidney tissue, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17984-1-AP targets AIF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIF fusion protein Ag12400
Full Name apoptosis-inducing factor, mitochondrion-associated, 1
Calculated molecular weight 609 aa, 66 kDa
Observed molecular weight 67 kDa, 57 kDa
GenBank accession numberBC111065
Gene symbol AIFM1
Gene ID (NCBI) 9131
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).

Protocols

Product Specific Protocols
WB protocol for AIF antibody 17984-1-APDownload protocol
IHC protocol for AIF antibody 17984-1-APDownload protocol
IF protocol for AIF antibody 17984-1-APDownload protocol
IP protocol for AIF antibody 17984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Invest Ophthalmol Vis Sci

Selective Serotonin Reuptake Inhibitors Aggravate Depression-Associated Dry Eye Via Activating the NF-κB Pathway.

Authors - Xiaozhao Zhang
humanWB

Int J Mol Sci

Proteomics Analysis of Tangeretin-Induced Apoptosis through Mitochondrial Dysfunction in Bladder Cancer Cells.

Authors - Jen-Jie Lin
humanWB

Chem Biol Interact

O-Alkylated derivatives of quercetin induce apoptosis of MCF-7 cells via a caspase-independent mitochondrial pathway.

Authors - Han Liao
humanWB,IF

J Cell Mol Med

Supplementary choline attenuates olive oil lipid emulsion-induced enterocyte apoptosis through suppression of CELF1/AIF pathway.

Authors - Jun-Kai Yan

Biochim Biophys Acta

Quantitative proteomics in Friedreich's ataxia B-lymphocytes: A valuable approach to decipher the biochemical events responsible for pathogenesis.

Authors - Lorène Télot
humanWB

Biochem Biophys Res Commun

XPO1-mediated nuclear export of RNF146 protects from angiotensin II-induced endothelial cellular injury.

Authors - Zhiyong Sheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Praveen (Verified Customer) (05-17-2019)

Excellent Antibody

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
AIF Antibody Western Blot, validation ( dilution) in AC16 (Cat no:17984-1-AP)