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  • KD/KO Validated

AIF Polyclonal antibody

AIF Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 17984-1-AP


AIF, AIFM1, Cell and organelle markers, PDCD8

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17984-1-AP targets AIF in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIF fusion protein Ag12400
Full Name apoptosis-inducing factor, mitochondrion-associated, 1
Calculated Molecular Weight 609 aa, 66 kDa
Observed Molecular Weight 67 kDa, 57 kDa
GenBank Accession NumberBC111065
Gene Symbol AIFM1
Gene ID (NCBI) 9131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID: 25101006).


Product Specific Protocols
WB protocol for AIF antibody 17984-1-APDownload protocol
IHC protocol for AIF antibody 17984-1-APDownload protocol
IF protocol for AIF antibody 17984-1-APDownload protocol
IP protocol for AIF antibody 17984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Rep

Newly synthesized AIFM1 determines the hypersensitivity of T lymphocytes to STING activation-induced cell apoptosis

Authors - Wangsheng Ji
  • KD Validated

Oxid Med Cell Longev

miRNA-27a Transcription Activated by c-Fos Regulates Myocardial Ischemia-Reperfusion Injury by Targeting ATAD3a.

Authors - Yandong Bao


Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.

Authors - Maxence Le Vasseur

PLoS Pathog

GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.

Authors - Xuewu Zhang

J Med Chem

Anticancer Effects of Honokiol via Mitochondrial Dysfunction Are Strongly Enhanced by the Mitochondria-Targeting Carrier Berberine.

Authors - Xiaojia Shi

J Agric Food Chem

β-Sitosterol-D-glucoside from sweet potato exerts an anti-breast cancer activity by upregulating microRNA-10a and PI3K/Akt signaling pathway.

Authors - Heshan Xu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tom (Verified Customer) (11-13-2020)

HeLa cells permeablisied (0.1% Triton X-100 in PBS) then blocked (1% BSA in PBS). Cells incubated with primary antibody (1:100) for 1 hour, 3xPBS prior to 1 hour incubation with goat anti-rabbit 488 Alexa Fluor secondary antibody.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HeLa
AIF Antibody Immunofluorescence validation (1:100 dilution) in HeLa (Cat no:17984-1-AP)

Praveen (Verified Customer) (05-17-2019)

Excellent Antibody

  • Applications: Western Blot,
  • Cell Tissue Type: AC16
AIF Antibody Western Blot, validation ( dilution) in AC16 (Cat no:17984-1-AP)