|Positive WB detected in||HeLa cells, Jurkat cells, A549 cells, rat kidney tissue, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
17984-1-AP targets AIF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AIF fusion protein Ag12400|
|Full Name||apoptosis-inducing factor, mitochondrion-associated, 1|
|Calculated molecular weight||609 aa, 66 kDa|
|Observed molecular weight||67 kDa, 57 kDa|
|GenBank accession number||BC111065|
|Gene ID (NCBI)||9131|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).
|Product Specific Protocols|
|WB protocol for AIF antibody 17984-1-AP||Download protocol|
|IHC protocol for AIF antibody 17984-1-AP||Download protocol|
|IF protocol for AIF antibody 17984-1-AP||Download protocol|
|IP protocol for AIF antibody 17984-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (11-13-2020)
HeLa cells permeablisied (0.1% Triton X-100 in PBS) then blocked (1% BSA in PBS). Cells incubated with primary antibody (1:100) for 1 hour, 3xPBS prior to 1 hour incubation with goat anti-rabbit 488 Alexa Fluor secondary antibody.
Praveen (Verified Customer) (05-17-2019)