Validation Data Gallery
|Positive WB detected in||HeLa cells, Jurkat cells, A549 cells, rat kidney tissue, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17984-1-AP targets AIF in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AIF fusion protein Ag12400|
|Full Name||apoptosis-inducing factor, mitochondrion-associated, 1|
|Calculated molecular weight||609 aa, 66 kDa|
|Observed molecular weight||67 kDa, 57 kDa|
|GenBank accession number||BC111065|
|Gene ID (NCBI)||9131|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).
|Product Specific Protocols|
|WB protocol for AIF antibody 17984-1-AP||Download protocol|
|IHC protocol for AIF antibody 17984-1-AP||Download protocol|
|IF protocol for AIF antibody 17984-1-AP||Download protocol|
|IP protocol for AIF antibody 17984-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells.
GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.
J Med Chem
Anticancer Effects of Honokiol via Mitochondrial Dysfunction Are Strongly Enhanced by the Mitochondria-Targeting Carrier Berberine.
Mol Nutr Food Res
Diosgenin Protects Against Kidney Injury and Mitochondrial Apoptosis Induced by 3-MCPD Through the Regulation of ER Stress, Ca2+ Homeostasis, and Bcl2 Expression.
Mitochondrial Glrx2 Knockout Augments Acetaminophen-Induced Hepatotoxicity in Mice
Selenoprotein T protects against cisplatin-induced acute kidney injury through suppression of oxidative stress and apoptosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Tom (Verified Customer) (11-13-2020)
HeLa cells permeablisied (0.1% Triton X-100 in PBS) then blocked (1% BSA in PBS). Cells incubated with primary antibody (1:100) for 1 hour, 3xPBS prior to 1 hour incubation with goat anti-rabbit 488 Alexa Fluor secondary antibody.
Praveen (Verified Customer) (05-17-2019)