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AKT1 Antibody 82 Publications

Rabbit Polyclonal| Catalog number: 10176-2-AP

Featured Product KD/KO validated

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Tested Applications
Applications:
ELISA, FC, IF, IHC, IP, WB
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Images See all

10176-2-AP;HeLa cells

HeLa cells were subjected to SDS PAGE followed by western blot with 10176-2-AP(AKT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

10176-2-AP;HeLa cells

siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.

10176-2-AP;HeLa cells

IP Result of anti-AKT1 (IP:10176-2-AP, 3ug; Detection:10176-2-AP 1:500) with HeLa cells lysate 2500ug.

10176-2-AP;human ovary tumor tissue

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP( AKT1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

10176-2-AP;human ovary tumor tissue

Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP( AKT1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

10176-2-AP;HeLa cells

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10176-2-AP(AKT1 antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

10176-2-AP;HeLa cells

1X10^6 HeLa cells were stained with 0.2ug AKT1 antibody (10176-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, zebrafish, A. sphaerocephala Krasch, hamster
Positive WB detected in:
HeLa cells, A549 cells, HeLa cells, HepG2 cells, human liver tissue, mouse testis tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human ovary tumor tissue, human breast cancer tissue, human cervical cancer tissue, human ovary tumor tissue
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AKT1 fusion protein ag0213
Full name:
v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight:
62 kDa
Observed molecular weight:
62 kDa
GenBank accession number:
BC000479
Gene ID (NCBI):
207
Gene symbol
AKT1
Synonyms
AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha
Background

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.


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