Validation Data Gallery
|Positive WB detected in||A549 cells, HepG2 cells, MCF-7 cells, HeLa cells, HEK-293 cells, NIH/3T3 cells, C6 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human ovary tumor tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10176-2-AP targets AKT in WB, IP, IHC, IF, FC, applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||Artemisia sphaerocephala Krasch, bovine, chicken, fish, goat, hamster, human, Megalobrama amblycephala, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||AKT fusion protein Ag0213|
|Full Name||v-akt murine thymoma viral oncogene homolog 1|
|Calculated molecular weight||56 kDa|
|Observed molecular weight||56-62 kDa|
|GenBank accession number||BC000479|
|Gene ID (NCBI)||207|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
|Product Specific Protocols|
|WB protocol for AKT antibody 10176-2-AP||Download protocol|
|IHC protocol for AKT antibody 10176-2-AP||Download protocol|
|IF protocol for AKT antibody 10176-2-AP||Download protocol|
|IP protocol for AKT antibody 10176-2-AP||Download protocol|
|FC protocol for AKT antibody 10176-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Med Sci Monit
Asian J Androl
Human tissue kallikrein-1 protects against the development of erectile dysfunction in a rat model of hyperhomocysteinemia.
Fish Physiol Biochem
Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala.
Biochem Biophys Res Commun
Long non-coding RNA FAM84B-AS promotes resistance of gastric cancer to platinum drugs through inhibition of FAM84B expression.
J Biomed Nanotechnol
Nano-Gold Loaded with Resveratrol Enhance the Anti-Hepatoma Effect of Resveratrol In Vitro and In Vivo.
Umbilical Cord Blood Mesenchymal Stem Cells Enhance Lipopolysaccharide-Induced IL-10 and IL-37 Production in THP-1 Cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hyopil (Verified Customer) (08-23-2021)
Worked well with western blotting
Azita (Verified Customer) (06-02-2021)
Western blot analysis using AKT polyclonal antibody in NSC34 cell line at dilution of 1:500.