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AKT Antibody 176 Publications

Rabbit Polyclonal| Catalog number: 10176-2-AP

Featured Product KD/KO validated

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

AKT Antibody WB MCF-7 cells 10176-2-AP

WB result of AKT antibody (10176-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT transfected MCF-7 cells

AKT Antibody WB HeLa cells 10176-2-AP

siRNA AKT1 result from Dr. Eva Martinez-Balibrea. Green:tubulin, Red:10176-2-AP, AKT1.

AKT Antibody WB HepG2 cells 10176-2-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10176-2-AP (AKT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

AKT Antibody IP HeLa cells 10176-2-AP

IP Result of anti-AKT1 (IP:10176-2-AP, 3ug; Detection:10176-2-AP 1:500) with HeLa cells lysate 2500ug.

AKT Antibody IHC human ovary tumor tissue 10176-2-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP( AKT1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

AKT Antibody IHC human ovary tumor tissue 10176-2-AP

Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10176-2-AP( AKT1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

AKT Antibody IF HeLa cells 10176-2-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 10176-2-AP(AKT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

AKT Antibody FC HeLa cells 10176-2-AP

1X10^6 HeLa cells were stained with 0.2ug AKT1 antibody (10176-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat, zebrafish, A. sphaerocephala Krasch, blunt snout bream, chicken, cow, hamster, swine
Positive WB detected in:
HepG2 cells, HeLa cells, human liver tissue, mouse testis tissue, A549 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human ovary tumor tissue, human cervical cancer tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:100-1:400
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
AKT fusion protein Ag0213
Full name:
v-akt murine thymoma viral oncogene homolog 1
Calculated molecular weight:
56 kDa
Observed molecular weight:
56-62 kDa
GenBank accession number:
BC000479
Gene ID (NCBI):
207
Gene symbol
AKT1
Synonyms
AKT, AKT1, AKT2, AKT3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

Background

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.


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