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AKT Monoclonal antibody
AKT Monoclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 60203-2-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse brain tissue, A549 cells, rat liver tissue, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue, human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60203-2-Ig is AKT Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||AKT fusion protein Ag16695|
|Full Name||v-akt murine thymoma viral oncogene homolog 1|
|Calculated molecular weight||56 kDa|
|Observed molecular weight||56-62 kDa|
|GenBank accession number||BC000479|
|Gene ID (NCBI)||207|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.
|Product Specific Protocols|
|WB protocol for AKT antibody 60203-2-Ig||Download protocol|
|IHC protocol for AKT antibody 60203-2-Ig||Download protocol|
|IF protocol for AKT antibody 60203-2-Ig||Download protocol|
|IP protocol for AKT antibody 60203-2-Ig||Download protocol|
|Click here to view our Standard Protocols|
Allogeneic adipose-derived stem cells suppress mTORC1 pathway in a murine model of systemic lupus erythematosus.
Evid Based Complement Alternat Med
Tetrandrine-Induced Autophagy in MDA-MB-231 Triple-Negative Breast Cancer Cell through the Inhibition of PI3K/AKT/mTOR Signaling.
Exp Cell Res
ITGA6 and RPSA synergistically promote pancreatic cancer invasion and metastasis via PI3K and MAPK signaling pathways.
Free Radic Biol Med
Neuroprotective effect of phosphocreatine on oxidative stress and mitochondrial dysfunction induced apoptosis in vitro and in vivo: Involvement of dual PI3K/Akt and Nrf2/HO-1 pathways.
Qing brick tea (QBT) aqueous extract protects monosodium glutamate-induced obese mice against metabolic syndrome and involves up-regulation Transcription Factor Nuclear Factor-Erythroid 2-Related Factor 2 (Nrf2) antioxidant pathway.
Oxid Med Cell Longev
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Janine (Verified Customer) (02-18-2019)
Yielded clear and distinguishable bands at the expected size of ~60kDa after 10 sec exposure using enhanced chemiluminescence.