Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, mouse brain tissue, ROS1728 cells, LNCaP cells, Hela cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60203-2-Ig targets AKT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, rabbit, chicken, hamster, sheep, goat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16695 Product name: Recombinant human AKT protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-224 aa of BC000479 Sequence: MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDVDQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQTVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKVILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDRLC Predict reactive species |
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 56-62 kDa |
| GenBank Accession Number | BC000479 |
| Gene Symbol | AKT1 |
| Gene ID (NCBI) | 207 |
| RRID | AB_10912803 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P31749 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AKT antibody 60203-2-Ig | Download protocol |
| IHC protocol for AKT antibody 60203-2-Ig | Download protocol |
| IP protocol for AKT antibody 60203-2-Ig | Download protocol |
| WB protocol for AKT antibody 60203-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-phospho-AKT (Ser473) antibody (Cat# 60203-2-Ig) has accumulated 897 citations across diverse research areas. Publications appear in high-impact journals including Nature Metabolism, Nature Communications, Cell Metabolism, Cell Reports, Advanced Science, Cell Death & Disease, Cancer Letters, Free Radical Biology and Medicine, Theranostics, Phytomedicine, and Journal of Nanobiotechnology. Research fields span oncology (tumor progression, chemoresistance, immunotherapy), neurodegeneration, metabolic disorders (diabetes, obesity), ferroptosis, autophagy, inflammation, cardiovascular disease, stem cell biology, and biomaterials/tissue engineering.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Targeting cancer cell plasticity by HDAC inhibition to reverse EBV-induced dedifferentiation in nasopharyngeal carcinoma. | ||
Signal Transduct Target Ther Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis | ||
Signal Transduct Target Ther Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells | ||
J Hematol Oncol Exosomes released by hepatocarcinoma cells endow adipocytes with tumor-promoting properties. | ||
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Adv Mater Silk fibroin and sericin differentially potentiate the paracrine and regenerative functions of stem cells through multiomics analysis |
Reviews
What customers say
Users consistently rate this antibody 4–5 stars across Western Blot, IHC, IF, and IP applications. Reviewers report clear, specific bands at the expected ~60 kDa size with dilutions ranging from 1:500 to 1:5000. It performs well on diverse samples including mouse brain lysate, RPE-1 cells, Caco-2 cells, and animal colon tissue. Overall, customers describe it as a reliable and high-performing reagent for detecting endogenous AKT.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (01-08-2026) | Antibodies are very wonderful, works very amazingly!
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Sai Sindhura (Verified Customer) (01-08-2026) | AKT works very good for IHC
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Areen (Verified Customer) (12-15-2025) | VERY GOOD ANTIBODY
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A (Verified Customer) (07-15-2025) | Used for caco2 cells and animal colon tissue
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C (Verified Customer) (06-25-2025) | A reliable Ab to detect endo Akt.
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Janine (Verified Customer) (02-18-2019) | Yielded clear and distinguishable bands at the expected size of ~60kDa after 10 sec exposure using enhanced chemiluminescence.
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