- Featured Product
- KD/KO Validated
AKT Monoclonal antibody
AKT Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat and More (4)
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
250
CloneNo.
2C5D1
Cat no : 60203-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, rat liver tissue, mouse brain tissue, HEK-293 cells, NIH/3T3 cells, RAW 264.7 cells, ROS1728 cells, LNCaP cells, Hela cells, Jurkat cells, HSC-T6 cells, PC-12 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 244 publications below |
IHC | See 4 publications below |
IF | See 5 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
60203-2-Ig targets AKT in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine, chicken, hamster, sheep |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AKT fusion protein Ag16695 |
Full Name | v-akt murine thymoma viral oncogene homolog 1 |
Calculated molecular weight | 56 kDa |
Observed molecular weight | 56-62 kDa |
GenBank accession number | BC000479 |
Gene symbol | AKT1 |
Gene ID (NCBI) | 207 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for AKT antibody 60203-2-Ig | Download protocol |
IHC protocol for AKT antibody 60203-2-Ig | Download protocol |
IF protocol for AKT antibody 60203-2-Ig | Download protocol |
IP protocol for AKT antibody 60203-2-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A Progesterone activates GPR126 to promote breast cancer development via the Gi pathway. | ||
Theranostics Interleukin 33-mediated inhibition of A-type K+ channels induces sensory neuronal hyperexcitability and nociceptive behaviors in mice. | ||
Free Radic Biol Med The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro | ||
J Med Chem Persistent Degradation of HER2 Protein by Hybrid nanoPROTAC for Programmed Cell Death | ||
J Invest Dermatol Keratinocyte growth factor-2 regulates signal-transducing adaptor protein-2-mediated signal transducer and activator of transcription 3 signaling and reduces skin scar formation. | ||
Cell Death Discov Cancer-associated fibroblast-secreted IGFBP7 promotes gastric cancer by enhancing tumor associated macrophage infiltration via FGF2/FGFR1/PI3K/AKT axis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Janine (Verified Customer) (02-18-2019) | Yielded clear and distinguishable bands at the expected size of ~60kDa after 10 sec exposure using enhanced chemiluminescence.
|