Phospho-AKT (Ser473) Polyclonal antibody

Phospho-AKT (Ser473) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat and More (2)





Cat no : 28731-1-AP


AKT, AKT1, Phospho-AKT (Ser473), PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

Tested Applications

Positive WB detected inCalyculin A treated PC-3 cells, PDGF treated NIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28731-1-AP targets Phospho-AKT (Ser473) in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-akt murine thymoma viral oncogene homolog 1
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_005163
Gene Symbol AKT1
Gene ID (NCBI) 207
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA (PubMed:20333297). This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation (PubMed:9512493). Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1 (PubMed:21464307, PubMed:8978681). AKT1 Thr-308 and Ser-473 is Phosphorylated by IKBKE and TBK1 (PubMed:15718470, PubMed:18456494, PubMed:20481595, PubMed:8978681). Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A) (PubMed:14761976). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells (PubMed:17013611). Ser-473 phosphorylation is enhanced by signaling through activated FLT3 (By similarity). Ser-473 is dephosphorylated by PHLPP (PubMed:28147277) and Ser-473 by PP2A phosphatase (PubMed:21329884).


Product Specific Protocols
WB protocol for Phospho-AKT (Ser473) antibody 28731-1-APDownload protocol
Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Itziar (Verified Customer) (06-27-2022)

This antibody works very well with even little protein quantities

  • Applications: Western Blot
  • Primary Antibody Dilution: %5 BSA in TBST
  • Cell Tissue Type: human pancreatic beta cells and Jurkat