AKT3 Polyclonal antibody

AKT3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21641-1-AP


AKT3, PKB GAMMA, PKBG, PRKBG, Protein kinase Akt 3, Protein kinase B gamma, RAC gamma, RAC PK gamma, STK 2

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21641-1-AP is AKT3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKT3 fusion protein Ag16298
Full Name v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Calculated Molecular Weight 465 aa, 54 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC121154
Gene Symbol AKT3
Gene ID (NCBI) 10000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKT3, also named as PKBG, is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas.


Product Specific Protocols
WB protocol for AKT3 antibody 21641-1-APDownload protocol
IHC protocol for AKT3 antibody 21641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Oncol

Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer.

Authors - Yi-Yuan Sun

J Cell Mol Med

EGR1 interacts with DNMT3L to inhibit the transcription of miR-195 and plays an anti-apoptotic role in the development of gastric cancer.

Authors - Yang Yang

Clin Epigenetics

Alterations of DNA methylation were associated with the rapid growth of cortisol-producing adrenocortical adenoma during pregnancy.

Authors - Chuan Wang

Cancer Cell Int

NEK7 promotes gastric cancer progression as a cell proliferation regulator.

Authors - Yi-Ke Li

Exp Hematol Oncol

YTHDF1 promotes hepatocellular carcinoma progression via activating PI3K/AKT/mTOR signaling pathway and inducing epithelial-mesenchymal transition.

Authors - Xiangyuan Luo

Sci Rep

Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways.

Authors - Jie Liu