AKT3 Polyclonal antibody

AKT3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21641-1-AP

Synonyms

AKT3, PKB GAMMA, PKBG, PRKBG, Protein kinase Akt 3, Protein kinase B gamma, RAC gamma, RAC PK gamma, STK 2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21641-1-AP is AKT3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKT3 fusion protein Ag16298
Full Name v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Calculated Molecular Weight 465 aa, 54 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC121154
Gene Symbol AKT3
Gene ID (NCBI) 10000
RRIDAB_10733649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKT3, also named as PKBG, is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. AKT3 is a key modulator of several tumors like melanoma, glioma and ovarian cancer. Active AKT3 increases progressively during melanoma tumor progression with highest levels present in advanced-stage metastatic melanomas.

Protocols

Product Specific Protocols
WB protocol for AKT3 antibody 21641-1-APDownload protocol
IHC protocol for AKT3 antibody 21641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Long Non-coding RNA AK025387 Promotes Cell Migration and Invasion of Gastric Cancer.

Authors - Yi-Yuan Sun
humanWB

J Cell Mol Med

EGR1 interacts with DNMT3L to inhibit the transcription of miR-195 and plays an anti-apoptotic role in the development of gastric cancer.

Authors - Yang Yang
humanIHC

Clin Epigenetics

Alterations of DNA methylation were associated with the rapid growth of cortisol-producing adrenocortical adenoma during pregnancy.

Authors - Chuan Wang
humanWB

Cancer Cell Int

NEK7 promotes gastric cancer progression as a cell proliferation regulator.

Authors - Yi-Ke Li
humanWB

Exp Hematol Oncol

YTHDF1 promotes hepatocellular carcinoma progression via activating PI3K/AKT/mTOR signaling pathway and inducing epithelial-mesenchymal transition.

Authors - Xiangyuan Luo
humanWB

Sci Rep

Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways.

Authors - Jie Liu