Albumin Polyclonal antibody
Albumin Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF,ELISA
Conjugate
Unconjugated
Cat no : 16475-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human blood tissue, human plasma tissue, mouse serum tissue, mouse liver tissue, HepG2 cells |
Positive IP detected in | human plasma tissue |
Positive IHC detected in | human liver tissue, human spleen tissue, human heart tissue, mouse liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse liver tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 29 publications below |
IHC | See 3 publications below |
IF | See 14 publications below |
Product Information
16475-1-AP targets Albumin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Albumin fusion protein Ag9569 |
Full Name | albumin |
Calculated molecular weight | 609 aa, 69 kDa |
Observed molecular weight | 66 kDa |
GenBank accession number | BC034023 |
Gene symbol | ALB |
Gene ID (NCBI) | 213 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Albumin antibody 16475-1-AP | Download protocol |
IHC protocol for Albumin antibody 16475-1-AP | Download protocol |
IF protocol for Albumin antibody 16475-1-AP | Download protocol |
IP protocol for Albumin antibody 16475-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Life Sci Blocking CXCR3 with AMG487 ameliorates the blood-retinal barrier disruption in diabetic mice through anti-oxidative. | ||
Cell Death Dis Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-ɑvβ6 integrin in liver fibrosis. | ||
Cell Death Dis Imbalance in mitochondrial dynamics induced by low PGC-1α expression contributes to hepatocyte EMT and liver fibrosis. | ||
Cell Death Dis Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease. | ||
bioRxiv Detection of Viral RNA Fragments in Human iPSC-Cardiomyocytes following Treatment with Extracellular Vesicles from SARS-CoV-2 Coding-Sequence-Overexpressing Lung Epithelial Cells. |