Validation Data Gallery
|Positive WB detected in||human blood tissue, human plasma, mouse serum, HepG2 cells, mouse liver tissue, rat liver|
|Positive IP detected in||human plasma tissue|
|Positive IHC detected in||human liver tissue, human spleen tissue, human heart tissue, mouse liver tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse liver tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16475-1-AP targets Albumin in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Albumin fusion protein Ag9569|
|Calculated molecular weight||609 aa, 69 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||BC034023|
|Gene ID (NCBI)||213|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)
|Product Specific Protocols|
|WB protocol for Albumin antibody 16475-1-AP||Download protocol|
|IHC protocol for Albumin antibody 16475-1-AP||Download protocol|
|IF protocol for Albumin antibody 16475-1-AP||Download protocol|
|IP protocol for Albumin antibody 16475-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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