Albumin Polyclonal antibody

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  WB analysis of HepG2 using 16475-1-AP

  WB result of Albumin antibody (16475-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Albumin transfected HepG2 cells.

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  WB analysis of human plasma using 16475-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 16475-1-AP (ALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16475-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse serum using 16475-1-AP

  mouse serum were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 16475-1-AP

  IP result of anti-Albumin (IP:16475-1-AP, 4ug; Detection:16475-1-AP 1:10000) with human plasma lysate 4000ug.

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  IHC staining of human liver using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16475-1-AP (Albumin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 16475-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 16475-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 16475-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of L02 using 16475-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using Albumin antibody (16475-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Albumin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 16475-1-AP

Publications(180)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Synonyms

ALB, Serum albumin, HSA

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Tested Applications

• Positive WB detected in: human blood tissue, human plasma, mouse serum, HepG2 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: human liver tissue, mouse liver tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 105 publications below
• IHC: See 16 publications below
• IF: See 69 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

16475-1-AP targets Albumin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Albumin fusion protein Ag9569
• Full Name: albumin
• Calculated Molecular Weight: 609 aa, 69 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC034023
• Gene Symbol: Albumin
• Gene ID (NCBI): 213
• RRID: AB_2242567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P02768
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)

Publications

Species	Application	Title
human,mouse	WB	Science; Enterically derived high-density lipoprotein restrains liver injury through the portal vein.; Authors - Yong-Hyun Han
mouse	IF	Signal Transduct Target Ther; MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration; Authors - Jinpeng Du
human,mouse	WB,IF	Signal Transduct Target Ther; The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease; Authors - Chandana Thimme Gowda
human, mouse	WB,IF	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	IHC	Immunity; The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation.; Authors - Wenjie Zhang
mouse	WB	Nat Cell Biol; Manganese regulation of COPII condensation controls circulating lipid homeostasis; Authors - Xiao Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (01-26-2024)

For western blot analysis, antibody worked well at dilution of 1:25000 in 1 X TBST. For immunofluorescence, antibody worked well at dilution of 1:200 in 1 X PBS.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Mouse bone marrow

FH

Kamal (Verified Customer) (01-26-2024)

Liver tissues were well stained in provided dilution with 1X PBS.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse liver tissue