Albumin Polyclonal antibody

Albumin Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP

Conjugate

Unconjugated

Publications

62

Cat no : 16475-1-AP

Synonyms

ALB, albumin, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341

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• Validation Data Gallery

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  WB analysis using 16475-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 16475-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 16475-1-AP (ALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 16475-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse liver using 16475-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using 16475-1-AP (Albumin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse liver using 16475-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using 16475-1-AP (Albumin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IP experiment of human plasma using 16475-1-AP

  IP Result of anti-Albumin (IP:16475-1-AP, 4ug; Detection:16475-1-AP 1:10000) with human plasma lysate 4000ug.

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Tested Applications

• Positive WB detected in: human blood tissue, human plasma, mouse serum, HepG2 cells, mouse liver tissue, rat liver
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: human liver tissue, human spleen tissue, human heart tissue, mouse liver tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 40 publications below
• IHC: See 5 publications below
• IF: See 22 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

16475-1-AP targets Albumin in WB, IP, IHC, IF, CoIP applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Albumin fusion protein Ag9569
• Full Name: albumin
• Calculated molecular weight: 609 aa, 69 kDa
• Observed molecular weight: 66 kDa
• GenBank accession number: BC034023
• Gene symbol: ALB
• Gene ID (NCBI): 213
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)

Publications

Species	Application	Title
mouse	WB,IF	Life Sci; Blocking CXCR3 with AMG487 ameliorates the blood-retinal barrier disruption in diabetic mice through anti-oxidative.; Authors - Honggang Wang
mouse	WB	Endocrinology; Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.; Authors - Jiao Fu
mouse	IF	Cell Death Dis; Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-ɑvβ6 integrin in liver fibrosis.; Authors - Ting Xu
human	WB	Cell Death Dis; Imbalance in mitochondrial dynamics induced by low PGC-1α expression contributes to hepatocyte EMT and liver fibrosis.; Authors - Linzhong Zhang
mouse	IF	Cell Death Dis; Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease.; Authors - Ye Feng
human	WB	bioRxiv; Detection of Viral RNA Fragments in Human iPSC-Cardiomyocytes following Treatment with Extracellular Vesicles from SARS-CoV-2 Coding-Sequence-Overexpressing Lung Epithelial Cells.; Authors - Youjeong Kwon

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