Albumin Polyclonal antibody
Albumin Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16475-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human blood tissue, human plasma tissue, mouse serum tissue, mouse liver tissue, HepG2 cells |
| Positive IP detected in | human plasma tissue |
| Positive IHC detected in | human liver tissue, human spleen tissue, human heart tissue, mouse liver tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 24 publications below |
| IHC | See 3 publications below |
| IF | See 12 publications below |
Product Information
16475-1-AP targets Albumin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Albumin fusion protein Ag9569 |
| Full Name | albumin |
| Calculated molecular weight | 609 aa, 69 kDa |
| Observed molecular weight | 66 kDa |
| GenBank accession number | BC034023 |
| Gene symbol | ALB |
| Gene ID (NCBI) | 213 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Albumin antibody 16475-1-AP | Download protocol |
| IHC protocol for Albumin antibody 16475-1-AP | Download protocol |
| IF protocol for Albumin antibody 16475-1-AP | Download protocol |
| IP protocol for Albumin antibody 16475-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Life Sci Blocking CXCR3 with AMG487 ameliorates the blood-retinal barrier disruption in diabetic mice through anti-oxidative. | ||
Cell Death Dis Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-ɑvβ6 integrin in liver fibrosis. | ||
Cell Death Dis Imbalance in mitochondrial dynamics induced by low PGC-1α expression contributes to hepatocyte EMT and liver fibrosis. | ||
Cell Death Dis Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease. | ||
bioRxiv Detection of Viral RNA Fragments in Human iPSC-Cardiomyocytes following Treatment with Extracellular Vesicles from SARS-CoV-2 Coding-Sequence-Overexpressing Lung Epithelial Cells. |
