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Albumin Antibody 17 Publications

Rabbit Polyclonal| Catalog number: 16475-1-AP

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Con: 40 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

Albumin Antibody WB mouse liver tissue 16475-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours

Albumin Antibody WB human plasma tissue 16475-1-AP

human blood tissue were subjected to SDS PAGE followed by western blot with 16475-1-AP(ALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours

Albumin Antibody IHC human liver tissue 16475-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens).

Albumin Antibody IHC human liver tissue 16475-1-AP

Immunohistochemistry of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens).

Albumin Antibody IF mouse liver tissue 16475-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse liver tissue using 16475-1-AP (Albumin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Albumin Antibody IF mouse liver tissue 16475-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse liver tissue using 16475-1-AP (Albumin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Albumin Antibody IP human plasma tissue 16475-1-AP

IP Result of anti-Albumin (IP:16475-1-AP, 4ug; Detection:16475-1-AP 1:10000) with human plasma tissue lysate 4000ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
human blood tissue, human plasma tissue, mouse liver tissue, mouse serum tissue
Positive IP detected in:
human plasma tissue
Positive IHC detected in:
human liver tissue, human heart tissue, human spleen tissue, human liver cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse liver tissue
Recommended dilution:
WB : 1:5000-1:50000
IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Albumin fusion protein Ag9569
Full name:
albumin
Calculated molecular weight:
609aa,69 kDa
Observed molecular weight:
66 kDa
GenBank accession number:
BC034023
Gene ID (NCBI):
213
Gene symbol
ALB
Synonyms
ALB, albumin, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341

Background

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)


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