Albumin Polyclonal antibody

Albumin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16475-1-AP

Synonyms

ALB, albumin, GIG42, HSA, PRO0883, PRO0903, PRO1341, PRO1708, PRO2044, PRO2619, PRO2675, Serum albumin, UNQ696/PRO1341



Tested Applications

Positive WB detected inhuman blood tissue, human plasma tissue, mouse serum tissue, mouse liver tissue, HepG2 cells
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue, human spleen tissue, human heart tissue, mouse liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16475-1-AP targets Albumin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Albumin fusion protein Ag9569
Full Name albumin
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC034023
Gene symbol ALB
Gene ID (NCBI) 213
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)

Protocols

Product Specific Protocols
WB protocol for Albumin antibody 16475-1-APDownload protocol
IHC protocol for Albumin antibody 16475-1-APDownload protocol
IF protocol for Albumin antibody 16475-1-APDownload protocol
IP protocol for Albumin antibody 16475-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Life Sci

Blocking CXCR3 with AMG487 ameliorates the blood-retinal barrier disruption in diabetic mice through anti-oxidative.

Authors - Honggang Wang
mouseWB

Endocrinology

Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.

Authors - Jiao Fu
mouseIF

Cell Death Dis

Myofibroblast induces hepatocyte-to-ductal metaplasia via laminin-ɑvβ6 integrin in liver fibrosis.

Authors - Ting Xu
humanWB

Cell Death Dis

Imbalance in mitochondrial dynamics induced by low PGC-1α expression contributes to hepatocyte EMT and liver fibrosis.

Authors - Linzhong Zhang
mouseIF

Cell Death Dis

Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease.

Authors - Ye Feng
humanWB

bioRxiv

Detection of Viral RNA Fragments in Human iPSC-Cardiomyocytes following Treatment with Extracellular Vesicles from SARS-CoV-2 Coding-Sequence-Overexpressing Lung Epithelial Cells.

Authors - Youjeong Kwon