ALDH1L2 Polyclonal antibody

ALDH1L2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 21391-1-AP

Synonyms

ALDH1L2

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Validation Data Gallery

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  WB analysis using 21391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21391-1-AP (ALDH1L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21391-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21391-1-AP (ALDH1L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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  IHC staining of human heart using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 21391-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 21391-1-AP (ALDH1L2 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: human pancreas tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below

Product Information

21391-1-AP targets ALDH1L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ALDH1L2 fusion protein Ag16046
• Full Name: aldehyde dehydrogenase 1 family, member L2
• Calculated molecular weight: 923 aa, 102 kDa
• Observed molecular weight: 102 kDa, 89 kDa
• GenBank accession number: BC103935
• Gene symbol: ALDH1L2
• Gene ID (NCBI): 160428
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH1L2(Aldehyde dehydrogenase family 1 member L2) is also named as mitochondrial 10-FTHFDH, mtFDH and belongs to the aldehyde dehydrogenase family and ALDH1L subfamily. The ALDH1L2 gene has three transcriptional variants with the molecular weight of 102 kDa, 42 kDa and 89 kDa(PMID:19823103). It is a mitochondrial enzyme, a likely source of CO2 production from 10-formyltetrahydrofolate in mitochondria and playing an essential role in the distribution of one-carbon groups between the cytosolic and mitochondrial compartments of the cell(PMID: 20498374). It also has dehydrogenase/hydrolase activities similar to cytosolic FDH (ALDH1L1). The native ALDH1L2 has been reported to exist as a dimer or tetramer(PMID:7822273).

Publications

Species	Application	Title
mouse	WB	Mol Cell; Kynurenine importation by SLC7A11 propagates anti-ferroptotic signaling.; Authors - Alessandra Fiore
human	IHC	Sci Adv; G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.; Authors - Yang Zhang
human	WB	Nat Commun; Defective NADPH production in mitochondrial disease complex I causes inflammation and cell death.; Authors - Eduardo Balsa
human,mouse	WB,IHC	Free Radic Biol Med; Ursodeoxycholic acid protects against cisplatin-induced acute kidney injury and mitochondrial dysfunction through acting on ALDH1L2.; Authors - Yunwen Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (08-26-2019)

Works well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: liver, kidney, hippocampus

ALDH1L2 Antibody Western Blot, validation (1:1000 dilution) in liver, kidney, hippocampus (Cat no:21391-1-AP)