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  • KD/KO Validated

5 Lipoxygenase Polyclonal antibody

5 Lipoxygenase Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10021-1-Ig

Synonyms

5 Lipoxygenase, 5 LO, 5 LOX, 5LPG, ALOX5, arachidonate 5 lipoxygenase, LOG5



Tested Applications

Positive WB detected inDU 145 cells, HL-60 cells, K-562 cells, PC-3 cells, human placenta tissue
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10021-1-Ig targets 5 Lipoxygenase in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein
Full Name arachidonate 5-lipoxygenase
Calculated molecular weight 674 aa, 78 kDa
Observed molecular weight 78 kDa
GenBank accession numberBC130332
Gene symbol ALOX5
Gene ID (NCBI) 240
RRIDAB_513237
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

Protocols

Product Specific Protocols
WB protocol for 5 Lipoxygenase antibody 10021-1-IgDownload protocol
IF protocol for 5 Lipoxygenase antibody 10021-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Front Pharmacol

Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway

Authors - Tong Zhou
human,mouseWB,IHC,IF

Int Immunopharmacol

The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis

Authors - Wei-Min Hu
mouseWB

Biochem Pharmacol

PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.

Authors - Yunhui Xu
humanWB

J Biol Chem

Inhibition of 5-lipoxygenase selectively triggers disruption of c-Myc signaling in prostate cancer cells.

Authors - Sivalokanathan Sarveswaran
  • KD Validated
humanWB

PLoS One

MK591, a Second Generation Leukotriene Biosynthesis Inhibitor, Prevents Invasion and Induces Apoptosis in the Bone-Invading C4-2B Human Prostate Cancer Cells: Implications for the Treatment of Castration-Resistant, Bone-Metastatic Prostate Cancer.

Authors - Sivalokanathan Sarveswaran
ratIHC

Exp Cell Res

Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.

Authors - Fen Xu