5 Lipoxygenase Polyclonal antibody

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  WB analysis using 10021-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10021-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 10021-1-Ig

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 10021-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 10021-1-Ig

  Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• Featured Product
• KD/KO Validated

5 Lipoxygenase Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 10021-1-Ig

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ALOX5, 5LOX, 5-LO, 5LO, 5-lipoxygenase

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Tested Applications

• Positive WB detected in: DU 145 cells, HL-60 cells, K-562 cells, PC-3 cells, human placenta tissue
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Product Information

10021-1-Ig targets 5 Lipoxygenase in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: arachidonate 5-lipoxygenase
• Calculated Molecular Weight: 674 aa, 78 kDa
• Observed Molecular Weight: 70-78 kDa
• GenBank Accession Number: BC130332
• Gene Symbol: 5 Lipoxygenase
• Gene ID (NCBI): 240
• RRID: AB_513237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09917
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

Publications

Species	Application	Title
mouse	WB,IHC	Front Pharmacol; Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway; Authors - Tong Zhou
human,mouse	WB,IHC,IF	Int Immunopharmacol; The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis; Authors - Wei-Min Hu
mouse	WB	Biochem Pharmacol; PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.; Authors - Yunhui Xu
human	WB	J Biol Chem; Inhibition of 5-lipoxygenase selectively triggers disruption of c-Myc signaling in prostate cancer cells.; Authors - Sivalokanathan Sarveswaran ; KD Validated
human	WB	PLoS One; MK591, a Second Generation Leukotriene Biosynthesis Inhibitor, Prevents Invasion and Induces Apoptosis in the Bone-Invading C4-2B Human Prostate Cancer Cells: Implications for the Treatment of Castration-Resistant, Bone-Metastatic Prostate Cancer.; Authors - Sivalokanathan Sarveswaran
rat	IHC	Exp Cell Res; Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.; Authors - Fen Xu