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PLAP Polyclonal antibody

PLAP Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 18507-1-AP

Synonyms

ALP, ALPP, PALP, Placental alkaline phosphatase, PLAP, PLAP 1



Tested Applications

Positive WB detected inhuman placenta tissue, mouse liver tissue, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman placenta tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18507-1-AP is PLAP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLAP fusion protein Ag13361
Full Name alkaline phosphatase, placental (Regan isozyme)
Calculated molecular weight 58 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC009647
Gene symbol ALPP
Gene ID (NCBI) 250
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.

Protocols

Product Specific Protocols
WB protocol for PLAP antibody 18507-1-APDownload protocol
IHC protocol for PLAP antibody 18507-1-APDownload protocol
IP protocol for PLAP antibody 18507-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

ACS Biomater Sci Eng

6-Bromoindirubin-3'-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model.

Authors - Song Shen
mouseWB

Aging (Albany NY)

Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.

Authors - Jie Xu
humanWB

Amino Acids

New aspects of antiproliferative activity of 4-hydroxybenzyl isothiocyanate, a natural H2S-donor.

Authors - Halina Jurkowska
WB

J Dent

PMID: 32119967

Authors - Yunxia Zhang
mouseWB

Mol Cell Endocrinol

Long noncoding RNA ZFAS1 suppresses osteogenic differentiation of bone marrow-derived mesenchymal stem cells by upregulating miR-499-EPHA5 axis.

Authors - Jinhui Wu