AMH Polyclonal antibody

AMH Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14461-1-AP

Synonyms

AMH, anti Mullerian hormone, MIF, MIS

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Validation Data Gallery

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  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of rat ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14461-1-AP (AMH antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human ovary tumor using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14461-1-AP (AMH antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human testis using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14461-1-AP (AMH antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14461-1-AP (AMH antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 14461-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 14461-1-AP (AMH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse ovary using 14461-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (14461-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse ovary using 14461-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using AMH antibody (14461-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: mouse ovary tissue, human pancreas tissue, human ovary tumor tissue, human testis tissue, rat ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse ovary tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

14461-1-AP targets AMH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat, chicken, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AMH fusion protein Ag5670
• Full Name: anti-Mullerian hormone
• Calculated molecular weight: 59 kDa
• GenBank accession number: BC049194
• Gene symbol: AMH
• Gene ID (NCBI): 268
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a hormone that is best known for its production by fetal testes in mammals and as the inhibitor of Müllerian duct development in males. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve. The deduced protein sequence of the AMH monomer contains a 25 amino acid secretion specific signal peptide and a monomeric protein of 535 amino acids that, when glycosylated at two glycosylation sites, has a molecular weight of 65-70 kDa. This antibody is specific to AMH.

Publications

Species	Application	Title
chicken	WB	Theriogenology; miR-140-3p promotes follicle granulosa cell proliferation and steroid hormone synthesis via targeting AMH in chickens; Authors - Bei-Bei Zhang ; KD Validated
mouse	WB	Exp Cell Res; Proteomic analysis of mouse ovaries during the prepubertal stages.; Authors - Jiaqiang Xiong
		Hum Reprod; Peroxisome proliferator-activated receptor-γ coactivator 1α-mediated pathway as a possible therapeutic target in endometriosis.; Authors - Hisashi Kataoka
mouse	IHC	J Funct Biomater; Construction of Artificial Ovaries with Decellularized Porcine Scaffold and Its Elicited Immune Response after Xenotransplantation in Mice; Authors - Tong Wu
rat	WB	Basic Clin Pharmacol Toxicol; Effects of quercetin on ovarian function and regulation of the ovarian PI3K/Akt/FoxO3a signalling pathway and oxidative stress in a rat model of cyclophosphamide-induced premature ovarian failure; Authors - Shaoyan Zheng
goat	WB	Theriogenology; BMP6 regulates AMH expression via SMAD1/5/8 in goat ovarian granulosa cells; Authors - Lei Wang