AMOT Monoclonal antibody

• Featured Product
• KD/KO Validated

AMOT Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3H7B3

Cat no : 60156-1-Ig

Synonyms

KIAA1071, Angiomotin, 3H7B3

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Validation Data Gallery

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  WB analysis of SGC-7901 using 60156-1-Ig

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60156-1-Ig (AMOT Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 60156-1-Ig

  WB result of AMOT antibody (60156-1-Ig, 1:1000) with si-Control and si-AMOT transfected HEK293 cells.

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  IF Staining of mouse skeletal muscle using 60156-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using AMOT antibody (60156-1-Ig, Clone: 3H7B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: SGC-7901 cells, HEK-293 cells
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60156-1-Ig targets AMOT in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: peptide
• Full Name: angiomotin
• Calculated Molecular Weight: 118 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: NM_001113490
• Gene Symbol: AMOT
• Gene ID (NCBI): 154796
• RRID: AB_10644279
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. The most abundant expression was found in the placenta and skeletal muscle. AMOT has two isoforms with MW 130 kDa (p130) and 80 kDa (p80). This antibody only recognizes p130.