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  • KD/KO Validated

Amphiphysin Polyclonal antibody

Amphiphysin Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13379-1-AP

Synonyms

AMPH, AMPH1, Amphiphysin



Tested Applications

Positive WB detected inmouse brain tissue, mouse lung tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human pancreas cancer tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13379-1-AP targets Amphiphysin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Amphiphysin fusion protein Ag4204
Full Name amphiphysin
Calculated molecular weight 695 aa, 76 kDa
Observed molecular weight 115-125 kDa
GenBank accession numberBC034376
Gene symbol AMPH
Gene ID (NCBI) 273
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Amphiphysin (AMPH), a synaptic vesicle-associated protein that is highly concentrated in nerve terminal, is an autoantigen of Stiff-Man syndrome with breast cancer. Amphiphysin contains a N-terminal BAR (Bin1/Amphiphysin/Rv167) domain followed by an endocytosis domain, and a C-terminal SH3 domain. Amphiphysin has been proposed to function as a linker between the clathrin coat and dynamin in the endocytosis of synaptic vesicles. It may also participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. (PMID: 8552632; 8245793; 10559861)

Protocols

Product Specific Protocols
WB protocol for Amphiphysin antibody 13379-1-APDownload protocol
IHC protocol for Amphiphysin antibody 13379-1-APDownload protocol
IP protocol for Amphiphysin antibody 13379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Physiol Rep

Conditional ablation of the prorenin receptor in nephron progenitor cells results in developmental programming of hypertension.

Authors - Renfang Song
humanWB,IHC

J Cell Mol Med

AMPH1 functions as a tumour suppressor in ovarian cancer via the inactivation of PI3K/AKT pathway.

Authors - Yajun Chen
  • KD Validated
humanWB,FC,IHC

Lasers Med Sci

AMPH-1 is a tumor suppressor of lung cancer by inhibiting Ras-Raf-MEK-ERK signal pathway.

Authors - Haifeng Yang
  • KD Validated
humanWB,IHC

J Cancer

AMPH-1 is critical for breast cancer progression.

Authors - Yajun Chen
  • KD Validated
mouseIF

Am J Physiol Renal Physiol

Renal blood flow and oxygenation drive nephron progenitor differentiation.

Authors - Christopher Rymer
mouseIF

PLoS One

Stromally Expressed β-Catenin Modulates Wnt9b Signaling in the Ureteric Epithelium.

Authors - Felix J Boivin