APC2 Polyclonal antibody

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  WB analysis using 13559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13559-1-AP (APC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13559-1-AP (APC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 13559-1-AP

  IP result of anti-APC2 (IP:13559-1-AP, 4ug; Detection:13559-1-AP 1:300) with MCF-7 cells lysate 3200ug.

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  IHC staining of human breast cancer using 13559-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13559-1-AP (APC2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13559-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13559-1-AP (APC2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 13559-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using APC2 antibody (13559-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

APC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13559-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ANAPC 2, ANAPC2, Anaphase-promoting complex subunit 2

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Product Information

13559-1-AP targets APC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APC2 fusion protein Ag4427
• Full Name: anaphase promoting complex subunit 2
• Calculated Molecular Weight: 822 aa, 94 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC032503
• Gene Symbol: APC2
• Gene ID (NCBI): 29882
• RRID: AB_2227055
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJX6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
Human	WB	Nat Commun; The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.; Authors - Kyungsoo Ha
mouse,human	WB,IF	FASEB J; APC2CDH1 negatively regulates agrin signaling by promoting the ubiquitination and proteolytic degradation of DOK7.; Authors - Aizhong Chen ; KD Validated
human	WB	FEBS J; The MAD2B-APC/C-MDM2 axis mediates acute kidney injury by modulating p53; Authors - Zhe Hao
mouse	IF	Nat Commun; CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I; Authors - Cheng-Jie Zhou
human	WB	Adv Sci (Weinh); CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC; Authors - Qi Li