Angiogenin Polyclonal antibody

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  WB analysis of mouse liver using 18302-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18302-1-AP (Angiogenin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human milk using 18302-1-AP

  human milk were subjected to SDS PAGE followed by western blot with 18302-1-AP (Angiogenin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 18302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18302-1-AP (Angiogenin antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 18302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18302-1-AP (Angiogenin antibody at dilution of 1:100 (under 40x lens).

Angiogenin Polyclonal Antibody for WB, IHC, ELISA

Cat No. 18302-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

ALS9, ANG, Angiogenin, HEL168, Ribonuclease 5, RNase 5, RNASE4, RNASE5

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Tested Applications

• Positive WB detected in: mouse liver tissue, human milk tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

18302-1-AP targets Angiogenin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: angiogenin, ribonuclease, RNase A family, 5
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC054880
• Gene Symbol: Angiogenin
• Gene ID (NCBI): 283
• RRID: AB_2274028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P03950
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC. It is a 17 kDa precursor which is cleaved to generate the 14 kDa mature protein.

Publications

Species	Application	Title
mouse	WB	Cell Rep Med; Hyperglycemia-regulated tRNA-derived fragment tRF-3001a propels neurovascular dysfunction in diabetic mice; Authors - Jun-Ya Zhu
human	IF	Theranostics; Transfer RNA derived fragment, tRF-Glu-CTC, aggravates the development of neovascular age-related macular degeneration; Authors - Yu Liang
mouse	WB	Neurosci Bull; The Circadian System Is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke; Authors - Yuxing Zhang
human	WB	FASEB J; MicroRNA-3064-5p sponged by MALAT1 suppresses angiogenesis in human hepatocellular carcinoma by targeting the FOXA1/CD24/Src pathway.; Authors - Pei Zhang
human	WB	Front Oncol; Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer.; Authors - Hui Tang
human	IHC	Front Med (Lausanne); Molecular and clinical characterization of ANG expression in gliomas and its association with tumor-related immune response; Authors - Jin Wang