Tested Applications
| Positive WB detected in | mouse liver tissue, human milk tissue |
| Positive IHC detected in | human liver tissue, human Hepatocellular carcinoma Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18302-1-AP targets Angiogenin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | angiogenin, ribonuclease, RNase A family, 5 |
| Calculated Molecular Weight | 17 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC054880 |
| Gene Symbol | Angiogenin |
| Gene ID (NCBI) | 283 |
| RRID | AB_2274028 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P03950 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC. It is a 17 kDa precursor which is cleaved to generate the 14 kDa mature protein.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Angiogenin antibody 18302-1-AP | Download protocol |
| WB protocol for Angiogenin antibody 18302-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Angiogenin (ANG) antibody (Cat# 18302-1-AP) has accumulated 18 citations across journals including Theranostics, Cell Reports Medicine, Cell Death & Disease, Cancer Letters, Free Radical Biology and Medicine, FASEB Journal, Life Sciences, International Immunopharmacology, and Heliyon. Research fields span angiogenesis, oncology (breast, liver, thyroid, colorectal, glioma), neurovascular biology, diabetes, reproductive medicine, stem cell therapy, and ferroptosis.
| Species | Application | Title |
|---|---|---|
Theranostics Transfer RNA derived fragment, tRF-Glu-CTC, aggravates the development of neovascular age-related macular degeneration | ||
Cell Rep Med Hyperglycemia-regulated tRNA-derived fragment tRF-3001a propels neurovascular dysfunction in diabetic mice | ||
Cell Death Dis DNMT2 inhibits anaplastic thyroid cancer progression by downregulating 5'tiRNA(Gly-GCC) production. | ||
Cancer Lett Electroacupuncture Facilitates Vascular Normalization by Inhibiting Glyoxalase1 in Endothelial Cells to Attenuate Glycolysis and Angiogenesis in Triple-Negative Breast Cancer | ||
Antioxidants (Basel) Ribonuclease Inhibitor 1 (RNH1) Regulates Sperm tsRNA Generation for Paternal Inheritance through Interacting with Angiogenin in the Caput Epididymis | ||
Free Radic Biol Med ANG secretion predisposes endothelial cells toward angiogenesis in FIN56-induced ferroptotic hepatocellular carcinoma via the BMP6/ID1 signaling pathway |
