Angiogenin Polyclonal antibody

Angiogenin Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 18302-1-AP

Synonyms

ALS9, ANG, Angiogenin, HEL168, Ribonuclease 5, RNase 5, RNASE4, RNASE5

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 18302-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18302-1-AP (Angiogenin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human milk using 18302-1-AP

  human milk were subjected to SDS PAGE followed by western blot with 18302-1-AP (Angiogenin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 18302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18302-1-AP (Angiogenin antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 18302-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18302-1-AP (Angiogenin antibody at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: mouse liver tissue, human milk tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

18302-1-AP targets Angiogenin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: angiogenin, ribonuclease, RNase A family, 5
• Calculated molecular weight: 17 kDa
• Observed molecular weight: 17 kDa
• GenBank accession number: BC054880
• Gene symbol: ANG
• Gene ID (NCBI): 283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiogenin (ANG), an angiogenic ribonuclease, is a member of the vertebrate-specific, secreted RNASE superfamily. Angiogenin, originally identified as a tumor angiogenic factor, was related with the growth and metastasis of numerous tumors. Angiogenin has been proposed as a permissive factor for angiogenesis induced by other angiogenic factors, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor, acidic fibroblast growth factor, and epidermal growth factor. Angiogenin production and secretion may be stimulated by hypoxia. Increased angiogenin serum levels have been associated with the incidence and severity of several human tumors, including HCC. It is a 17 kDa precursor which is cleaved to generate the 14 kDa mature protein.

Publications

Species	Application	Title
mouse	WB	Neurosci Bull; The Circadian System Is Essential for the Crosstalk of VEGF-Notch-mediated Endothelial Angiogenesis in Ischemic Stroke; Authors - Yuxing Zhang
human	WB	FASEB J; MicroRNA-3064-5p sponged by MALAT1 suppresses angiogenesis in human hepatocellular carcinoma by targeting the FOXA1/CD24/Src pathway.; Authors - Pei Zhang
human	WB	Front Oncol; Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer.; Authors - Hui Tang
rat	IHC	Life Sci; Sphingosine kinase 1/sphingosine 1-phosphate/sphingosine 1-phosphate receptor 1 pathway: A novel target of geniposide to inhibit angiogenesis.; Authors - Minghui Sun
rat	WB	Stem Cells Int; Bone Marrow-Derived Mesenchymal Stem Cells Repair Necrotic Pancreatic Tissue and Promote Angiogenesis by Secreting Cellular Growth Factors Involved in the SDF-1 α /CXCR4 Axis in Rats.; Authors - Daohai Qian
human	IHC	Cell Transplant; Transplantation of Human Adipose Stem Cells Using Acellular Human Amniotic Membrane Improves Angiogenesis in Injured Endometrial Tissue in a Rat Intrauterine Adhesion Model.; Authors - Xiao Han