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Angiopoietin 1 Polyclonal antibody

Angiopoietin 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 23302-1-AP


AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, Angiopoietin-1, ANGPT1, KIAA0003

Tested Applications

Positive WB detected inHUVEC cells, mouse liver tissue, HepG2 cells, mouse heart tissue, A375 cells, rat heart tissue, HeLa cells, K-562 cells, C6 cells, U-87 MG cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23302-1-AP is Angiopoietin 1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Angiopoietin 1 fusion protein Ag18829
Full Name angiopoietin 1
Calculated molecular weight 498 aa, 58 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC152411
Gene symbol ANGPT1
Gene ID (NCBI) 284
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.


Product Specific Protocols
WB protocol for Angiopoietin 1 antibody 23302-1-APDownload protocol
IHC protocol for Angiopoietin 1 antibody 23302-1-APDownload protocol
IF protocol for Angiopoietin 1 antibody 23302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Biomater Res

High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration

Authors - Di Sun

Mol Ther Nucleic Acids

Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.

Authors - Ying Wang

Br J Pharmacol

Canonical hedgehog signaling regulates hepatic stellate cell-mediated angiogenesis in liver fibrosis.

Authors - Feng Zhang

Cell Physiol Biochem

Neuregulin-1 Promotes Myocardial Angiogenesis in the Rat Model of Diabetic Cardiomyopathy.

Authors - Chun Gui

Front Oncol

Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer.

Authors - Hui Tang

Stem Cell Res Ther

MicroRNA-9 modified bone marrow-derived mesenchymal stem cells (BMSCs) repair severe acute pancreatitis (SAP) via inducing angiogenesis in rats.

Authors - Daohai Qian