Angiopoietin 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Angiopoietin 1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 23302-1-AP

Synonyms

AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, Angiopoietin-1, ANGPT1, KIAA0003

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Validation Data Gallery

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  WB analysis using 23302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 23302-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23302-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23302-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 23302-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 23302-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 23302-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 23302-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 23302-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HUVEC cells, mouse liver tissue, HepG2 cells, mouse heart tissue, A375 cells, rat heart tissue, HeLa cells, K-562 cells, C6 cells, U-87 MG cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 19 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

The immunogen of 23302-1-AP is Angiopoietin 1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Angiopoietin 1 fusion protein Ag18829
• Full Name: angiopoietin 1
• Calculated molecular weight: 498 aa, 58 kDa
• Observed molecular weight: 70 kDa
• GenBank accession number: BC152411
• Gene symbol: ANGPT1
• Gene ID (NCBI): 284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Publications

Species	Application	Title
human	WB	Biomater Res; High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration; Authors - Di Sun
human	WB,IF	Mol Ther Nucleic Acids; Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.; Authors - Ying Wang
mouse	WB	Br J Pharmacol; Canonical hedgehog signaling regulates hepatic stellate cell-mediated angiogenesis in liver fibrosis.; Authors - Feng Zhang
rat	WB	Cell Physiol Biochem; Neuregulin-1 Promotes Myocardial Angiogenesis in the Rat Model of Diabetic Cardiomyopathy.; Authors - Chun Gui
human	WB	Front Oncol; Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer.; Authors - Hui Tang
rat	WB	Stem Cell Res Ther; MicroRNA-9 modified bone marrow-derived mesenchymal stem cells (BMSCs) repair severe acute pancreatitis (SAP) via inducing angiogenesis in rats.; Authors - Daohai Qian