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Angiopoietin 1 Polyclonal antibody
Angiopoietin 1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 23302-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HUVEC cells, mouse liver tissue, HepG2 cells, mouse heart tissue, A375 cells, rat heart tissue, HeLa cells, K-562 cells, C6 cells, U-87 MG cells |
Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 19 publications below |
IHC | See 5 publications below |
IF | See 2 publications below |
Product Information
The immunogen of 23302-1-AP is Angiopoietin 1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Angiopoietin 1 fusion protein Ag18829 |
Full Name | angiopoietin 1 |
Calculated molecular weight | 498 aa, 58 kDa |
Observed molecular weight | 70 kDa |
GenBank accession number | BC152411 |
Gene symbol | ANGPT1 |
Gene ID (NCBI) | 284 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.
Protocols
Product Specific Protocols | |
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WB protocol for Angiopoietin 1 antibody 23302-1-AP | Download protocol |
IHC protocol for Angiopoietin 1 antibody 23302-1-AP | Download protocol |
IF protocol for Angiopoietin 1 antibody 23302-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Biomater Res High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration | ||
Mol Ther Nucleic Acids Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC. | ||
Br J Pharmacol Canonical hedgehog signaling regulates hepatic stellate cell-mediated angiogenesis in liver fibrosis. | ||
Cell Physiol Biochem Neuregulin-1 Promotes Myocardial Angiogenesis in the Rat Model of Diabetic Cardiomyopathy. | ||
Front Oncol Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer. | ||
Stem Cell Res Ther MicroRNA-9 modified bone marrow-derived mesenchymal stem cells (BMSCs) repair severe acute pancreatitis (SAP) via inducing angiogenesis in rats. |