Angiopoietin 1 Polyclonal antibody

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  WB analysis using 23302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 23302-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23302-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23302-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 23302-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 23302-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 23302-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Angiopoietin 1 antibody (23302-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

Angiopoietin 1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 23302-1-AP

Publications(29)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ANGPT1, angiopoietin1, ANG-1, ANG1, ANG 1

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Tested Applications

• Positive WB detected in: HUVEC cells, mouse liver tissue, HepG2 cells, mouse heart tissue, A375 cells, rat heart tissue, HeLa cells, K-562 cells, C6 cells, U-87 MG cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 27 publications below
• IF: See 2 publications below

Product Information

23302-1-AP targets Angiopoietin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Angiopoietin 1 fusion protein Ag18829
• Full Name: angiopoietin 1
• Calculated Molecular Weight: 498 aa, 58 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC152411
• Gene Symbol: Angiopoietin 1
• Gene ID (NCBI): 284
• RRID: AB_2879250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15389
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Publications

Species	Application	Title
human	WB	Biomater Res; High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration; Authors - Di Sun
human,rat	WB	Acta Biomater; Chemotactic Recruitment of Genetically Engineered Cell Membrane-Camouflaged Metal-Organic Framework Nanoparticles for Ischemic Osteonecrosis Treatment; Authors - Hongyi Jiang
human	WB,IF	Mol Ther Nucleic Acids; Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.; Authors - Ying Wang
human,mouse	WB	Neural Regen Res; Endorepellin downregulation promotes angiogenesis after experimental traumatic brain injury; Authors - Qian Zhang
human	WB	J Cell Mol Med; IGFBP-4 enhances VEGF-induced angiogenesis in a mouse model of myocardial infarction.; Authors - Da Wo
mouse	WB	Br J Pharmacol; Canonical hedgehog signaling regulates hepatic stellate cell-mediated angiogenesis in liver fibrosis.; Authors - Feng Zhang