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  • KD/KO Validated

ANP32B Antibody

ANP32B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 10843-1-AP

Synonyms

ANP32B, APRIL, PHAPI2, Silver stainable protein SSP29, SSP29



Tested Applications

Positive WB detected inHEK-293T cells, RAW 264.7 cells, HEK-293 cells, A431 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue, human pancreas tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10843-1-AP targets ANP32B in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse samples.

Reactivity human, mouse
Cited Species human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANP32B fusion protein Ag1274
Full Name acidic (leucine-rich) nuclear phosphoprotein 32 family, member B
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession number BC013003
Gene symbol ANP32B
Gene ID (NCBI) 10541
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).

Protocols

Product Specific Protocols
WB protocol for ANP32B antibody 10843-1-APDownload protocol
IHC protocol for ANP32B antibody 10843-1-APDownload protocol
IF protocol for ANP32B antibody 10843-1-APDownload protocol
IP protocol for ANP32B antibody 10843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Med Rep

Caspase-3‑resistant uncleavable form of acidic leucine-rich nuclear phosphoprotein 32B potentiates leukemic cell apoptosis.

Authors - Cai-Xia Li
humanWB

Biochem Biophys Res Commun

Acidic leucine-rich nuclear phosphoprotein 32 family member B (ANP32B) contributes to retinoic acid-induced differentiation of leukemic cells.

Authors - Yu Yun Y
mouseWB

PLoS One

Generation and characterization of the Anp32e-deficient mouse.

Authors - Reilly Patrick T PT
humanWB

J Virol

ANP32 proteins are essential for influenza virus replication in human cells.

Authors - Ecco Staller
  • KO Validated
humanWB,IHC

Cell Death Dis

ANP32B deficiency impairs proliferation and suppresses tumor progression by regulating AKT phosphorylation.

Authors - S Yang
  • KD Validated
humanWB

Blood Cancer J

Synergistic cytotoxicity of gemcitabine, clofarabine and edelfosine in lymphoma cell lines.

Authors - B C Valdez