ANP32CP Polyclonal antibody

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  WB analysis using 31298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31298-1-AP (ANP32CP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ANP32CP Polyclonal Antibody for WB, ELISA

Cat No. 31298-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

ANP32C, PP32R1, Tumorigenic protein pp32r1

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Tested Applications

• Positive WB detected in: DU 145 cells, LNCaP cells, PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31298-1-AP targets ANP32CP in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANP32CP fusion protein Ag35293
• Full Name: acidic (leucine-rich) nuclear phosphoprotein 32 family, member C
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27-30 kDa
• GenBank Accession Number: NM_012403.1
• Gene Symbol: ANP32C/PP32R1
• Gene ID (NCBI): 23520
• RRID: AB_3669935
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O43423
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ANP32CP (acidic nuclear phosphoprotein 32 family member C, pseudogene), also known as PP32R1. It is expected to be located in nucleus and perinuclear region of cytoplasm. Expressed in activated stem cells, such as mobilized CD34+ cells and cord blood CD34+ cells, but not in resting bone marrow CD34+ cells. Expressed in a variety of neoplastic cell lines, mainly in prostatic adenocarcinoma cell lines. Not expressed in normal prostatic tissue (PMID: 10086381). The calculated molecular weight of ANP32CP is 27 kDa. The protein could be the product of a pseudogene. Gene encoding ANP32CP is intronless suggesting that is pseudogene generated by retrotransposition.