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ACOX1 Polyclonal antibody

ACOX1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10957-1-AP

Synonyms

ACOX, ACOX1, AOX, PALMCOX, Palmitoyl CoA oxidase, SCOX



Tested Applications

Positive WB detected inmouse liver tissue, mouse kidney tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10957-1-AP targets ACOX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACOX1 fusion protein Ag1405
Full Name acyl-Coenzyme A oxidase 1, palmitoyl
Calculated molecular weight 74 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC010425
Gene symbol ACOX1
Gene ID (NCBI) 51
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

Protocols

Product Specific Protocols
WB protocol for ACOX1 antibody 10957-1-APDownload protocol
IHC protocol for ACOX1 antibody 10957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Am J Physiol Gastrointest Liver Physiol

Inhibition of soluble epoxide hydrolase ameliorates hyperhomocysteinemia-induced hepatic steatosis by enhancing β-oxidation of fatty acid in mice.

Authors - Liu Yao
humanWB

Hepatobiliary Pancreat Dis Int

miR-222 targets ACOX1, promotes triglyceride accumulation in hepatocytes.

Authors - Jing-Jie Wang
humanWB

Biochim Biophys Acta

A novel case of ACOX2 deficiency leads to recognition of a third human peroxisomal acyl-CoA oxidase.

Authors - Sacha Ferdinandusse
humanWB

Biosci Biotechnol Biochem

Effect and mechanism of ginsenoside Rg1-regulating hepatic steatosis in HepG2 cells induced by free fatty acid.

Authors - Yue Gao
ratWB

Biomed Environ Sci

Di (2-ethylhexyl) phthalate Disorders Lipid Metabolism via TYK2/STAT1 and Autophagy in Rats.

Authors - Yue Zhu Zhang
mouseWB,IF

Biochem Biophys Res Commun

Expressional studies of the aldehyde oxidase (AOX1) gene during myogenic differentiation in C2C12 cells.

Authors - Majid Rasool Kamli