|Positive WB detected in||mouse liver tissue, mouse kidney tissue, rat liver tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10957-1-AP targets ACOX1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACOX1 fusion protein Ag1405|
|Full Name||acyl-Coenzyme A oxidase 1, palmitoyl|
|Calculated molecular weight||74 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC010425|
|Gene ID (NCBI)||51|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).
Am J Physiol Gastrointest Liver Physiol
Inhibition of soluble epoxide hydrolase ameliorates hyperhomocysteinemia-induced hepatic steatosis by enhancing β-oxidation of fatty acid in mice.
Hepatobiliary Pancreat Dis Int
miR-222 targets ACOX1, promotes triglyceride accumulation in hepatocytes.
Biochim Biophys Acta
A novel case of ACOX2 deficiency leads to recognition of a third human peroxisomal acyl-CoA oxidase.
Melanoma Persister Cells Are Tolerant to BRAF/MEK Inhibitors via ACOX1-Mediated Fatty Acid Oxidation.
Biosci Biotechnol Biochem
Effect and mechanism of ginsenoside Rg1-regulating hepatic steatosis in HepG2 cells induced by free fatty acid.
Evid Based Complement Alternat Med
Dendrobium officinale Regulates Fatty Acid Metabolism to Ameliorate Liver Lipid Accumulation in NAFLD Mice.