ACOX1 Polyclonal antibody

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  WB analysis using 10957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10957-1-AP (ACOX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10957-1-AP (ACOX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10957-1-AP

  WB result of ACOX1 antibody (10957-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACOX1 transfected HepG2 cells.

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  WB analysis of mouse kidney using 10957-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10957-1-AP (ACOX1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10957-1-AP (ACOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10957-1-AP (ACOX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 10957-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ACOX1 antibody (10957-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

ACOX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10957-1-AP

Publications(111)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Peroxisomal acyl-CoA oxidase 1, A chain, Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, mouse kidney tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 92 publications below
• IHC: See 14 publications below
• IF: See 12 publications below

Product Information

10957-1-AP targets ACOX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACOX1 fusion protein Ag1405
• Full Name: acyl-Coenzyme A oxidase 1, palmitoyl
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 70-74kDa, 50 kDa, 22kDa
• GenBank Accession Number: BC010425
• Gene Symbol: ACOX1
• Gene ID (NCBI): 51
• RRID: AB_2221670
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15067
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	J Hematol Oncol; Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma; Authors - Yexin Tong
human	WB	Gastroenterology; Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.; Authors - Yuhong Luo
chicken	WB	Adv Sci (Weinh); α-Lipoic Acid Ameliorates Arsenic-Induced Lipid Disorders by Promoting Peroxisomal β-Oxidation and Reducing Lipophagy in Chicken Hepatocyte; Authors - Yangfei Zhao
mouse	WB	Mol Cell; Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.; Authors - Anyuan He ; KO Validated
mouse	WB	J Clin Invest; Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.; Authors - Hongsuk Park ; KO Validated
human	IF	EMBO J; Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.; Authors - Tatsuo Miyamoto