ACOX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACOX1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

37

Cat no : 10957-1-AP

Synonyms

ACOX, ACOX1, AOX, PALMCOX, Palmitoyl CoA oxidase, SCOX

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Validation Data Gallery

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  WB analysis using 10957-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10957-1-AP (ACOX1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 10957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10957-1-AP (ACOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10957-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10957-1-AP (ACOX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse kidney tissue, rat liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 34 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

10957-1-AP targets ACOX1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: chicken, human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACOX1 fusion protein Ag1405
• Full Name: acyl-Coenzyme A oxidase 1, palmitoyl
• Calculated molecular weight: 74 kDa
• Observed molecular weight: 50 kDa
• GenBank accession number: BC010425
• Gene symbol: ACOX1
• Gene ID (NCBI): 51
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).

Publications

Species	Application	Title
mouse	WB	Am J Physiol Gastrointest Liver Physiol; Inhibition of soluble epoxide hydrolase ameliorates hyperhomocysteinemia-induced hepatic steatosis by enhancing β-oxidation of fatty acid in mice.; Authors - Liu Yao
human	WB	Hepatobiliary Pancreat Dis Int; miR-222 targets ACOX1, promotes triglyceride accumulation in hepatocytes.; Authors - Jing-Jie Wang
human	WB	Biochim Biophys Acta; A novel case of ACOX2 deficiency leads to recognition of a third human peroxisomal acyl-CoA oxidase.; Authors - Sacha Ferdinandusse
human	WB	Cell Rep; Melanoma Persister Cells Are Tolerant to BRAF/MEK Inhibitors via ACOX1-Mediated Fatty Acid Oxidation.; Authors - Shensi Shen ; KD Validated
human	WB	Biosci Biotechnol Biochem; Effect and mechanism of ginsenoside Rg1-regulating hepatic steatosis in HepG2 cells induced by free fatty acid.; Authors - Yue Gao
rat	WB	Biomed Environ Sci; Di (2-ethylhexyl) phthalate Disorders Lipid Metabolism via TYK2/STAT1 and Autophagy in Rats.; Authors - Yue Zhu Zhang