|Positive WB detected in
|Positive IP detected in
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2400
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16932-1-AP targets AP1B1 in WB, IP, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|AP1B1 fusion protein Ag10417
|adaptor-related protein complex 1, beta 1 subunit
|Calculated molecular weight
|919 aa, 101 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.
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