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AP3B1 Polyclonal antibody

AP3B1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 13384-1-AP

Synonyms

ADTB3, ADTB3A, AP 3 complex subunit beta 1, AP3B1, Beta 3A adaptin, HPS, HPS2, PE



Tested Applications

Positive WB detected inmouse thymus tissue, COLO 320 cells
Positive IP detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

13384-1-AP targets AP3B1 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP3B1 fusion protein Ag4225
Full Name adaptor-related protein complex 3, beta 1 subunit
Calculated molecular weight 1094 aa, 121 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC038444
Gene symbol AP3B1
Gene ID (NCBI) 8546
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AP3B1 is the 140-kDa β3A subunit of the adaptor-related protein complex-3 (AP-3), a ubiquitous heterotetrameric complex that is localized to the trans-Golgi network and endosomes and is involved in protein trafficking to lysosomes or specialized endosomal-lysosomal organelles (PMID: 9182526; 9545220). This complex is composed of two lager subunits (δ and β3A or β3B), a medium subunit (μ3A or μ3B), and a small subunit (σ3A or σ3B). The absence of the β3A subunit (AP3B1) results in the loss of stability of AP3 and leads to degradation of μ3A, to which β3A is directly bound, while the other subunits are variably affected (PMID: 16507770). AP3B1 contains three main domains: the N-terminal head domain, the hinge, and the C-terminal ear domain. It has been reported as a target of IP(7)-mediated pyrophosphorylation (PMID: 19934039). Defects in AP3B1 are the cause of Hermansky-Pudlak syndrome type 2 (HPS2) (PMID: 10024875; 16507770).

Protocols

Product Specific Protocols
WB protocol for AP3B1 antibody 13384-1-APDownload protocol
IP protocol for AP3B1 antibody 13384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Haematologica

Defective AP-3-dependent VAMP8 trafficking impairs Weibel-Palade body exocytosis in Hermansky-Pudlak Syndrome type 2 blood outgrowth endothelial cells.

Authors - Ellie Karampini
humanWB

Stem Cell Reports

In Vitro Disease Modeling of Hermansky-Pudlak Syndrome Type 2 Using Human Induced Pluripotent Stem Cell-Derived Alveolar Organoids.

Authors - Yohei Korogi
humanWB

PLoS One

Cellular and molecular defects in a patient with Hermansky-Pudlak syndrome type 5.

Authors - Joshi Stephen

Mol Biol Cell

Adaptor protein-3 complex is required for Vangl2 trafficking and planar cell polarity of the inner ear.

Authors - Cristy Tower-Gilchrist
humanWB

Mol Biol Cell

Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells.

Authors - Zlatic Stephanie A SA
humanWB

Mol Biol Cell

Chemical-Genetic Disruption of Clathrin Function Spares Adaptor Complex 3-Dependent Endosome Vesicle Biogenesis.

Authors - Zlatic Stephanie A SA