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  • KD/KO Validated

AP3B1 Polyclonal antibody

AP3B1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13384-1-AP

Synonyms

ADTB3, ADTB3A, AP 3 complex subunit beta 1, AP3B1, Beta 3A adaptin, HPS, HPS2, PE



Tested Applications

Positive WB detected inA431 cells, mouse thymus tissue, COLO 320 cells, HeLa cells, HepG2 cells, SKOV-3 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13384-1-AP targets AP3B1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AP3B1 fusion protein Ag4225
Full Name adaptor-related protein complex 3, beta 1 subunit
Calculated molecular weight 1094 aa, 121 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC038444
Gene symbol AP3B1
Gene ID (NCBI) 8546
RRIDAB_2056499
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AP3B1 is the 140-kDa β3A subunit of the adaptor-related protein complex-3 (AP-3), a ubiquitous heterotetrameric complex that is localized to the trans-Golgi network and endosomes and is involved in protein trafficking to lysosomes or specialized endosomal-lysosomal organelles (PMID: 9182526; 9545220). This complex is composed of two larger subunits (δ and β3A or β3B), a medium subunit (μ3A or μ3B), and a small subunit (σ3A or σ3B). The absence of the β3A subunit (AP3B1) results in the loss of stability of AP3 and leads to degradation of μ3A, to which β3A is directly bound, while the other subunits are variably affected (PMID: 16507770). AP3B1 contains three main domains: the N-terminal head domain, the hinge, and the C-terminal ear domain. It has been reported as a target of IP(7)-mediated pyrophosphorylation (PMID: 19934039). Defects in AP3B1 are the cause of Hermansky-Pudlak syndrome type 2 (HPS2) (PMID: 10024875; 16507770).

Protocols

Product Specific Protocols
WB protocol for AP3B1 antibody 13384-1-APDownload protocol
IHC protocol for AP3B1 antibody 13384-1-APDownload protocol
IP protocol for AP3B1 antibody 13384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Misrouting of v-ATPase subunit V0a1 dysregulates lysosomal acidification in a neurodegenerative lysosomal storage disease model.

Authors - Maria B Bagh
humanWB

Cell Rep

Modeling of Fibrotic Lung Disease Using 3D Organoids Derived from Human Pluripotent Stem Cells.

Authors - Alexandros Strikoudis
  • KO Validated
WB

EMBO Rep

BioID reveals an ATG9A interaction with ATG13-ATG101 in the degradation of p62/SQSTM1-ubiquitin clusters.

Authors - Ashari R Kannangara
humanWB

J Neurosci

Quantitative proteomic and genetic analyses of the schizophrenia susceptibility factor dysbindin identify novel roles of the biogenesis of lysosome-related organelles complex 1.

Authors - Gokhale Avanti A
humanWB,IF

J Virol

Matrix Proteins of Nipah and Hendra Viruses Interact with Beta Subunits of AP-3 Complexes.

Authors - Weina Sun
  • KD Validated
humanWB,IF

J Biol Chem

BLOC-2, AP-3, and AP-1 proteins function in concert with Rab38 and Rab32 proteins to mediate protein trafficking to lysosome-related organelles.

Authors - Bultema Jarred J JJ
  • KD Validated