APAF1 Antibody 7 Publications

Rabbit Polyclonal| Catalog number: 21710-1-AP

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Freight/Packing

Con: 23 μg/150 μl

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Species specificity:
human, rat, mouse

Positive WB detected in:
HEK-293 cells, A549 cells, COLO 320 cells

Positive IP detected in:
HEK-293 cells

Positive IHC detected in:
human heart tissue

Positive IF detected in:
A549 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
apoptotic peptidase activating factor 1

Calculated molecular weight:
1248aa,142 kDa

Observed molecular weight:
130-142 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
APAF1

Synonyms
APAF 1, APAF1, CED4, DKFZp781B1145, KIAA0413
Background

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.


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