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APAF1 Polyclonal antibody

APAF1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21710-1-AP

Synonyms

APAF 1, APAF1, CED4, KIAA0413



Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, COLO 320 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21710-1-AP is APAF1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APAF1 fusion protein Ag16336
Full Name apoptotic peptidase activating factor 1
Calculated molecular weight 1248 aa, 142 kDa
Observed molecular weight 130-142 kDa
GenBank accession numberBC136532
Gene symbol APAF1
Gene ID (NCBI) 317
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Protocols

Product Specific Protocols
WB protocol for APAF1 antibody 21710-1-APDownload protocol
IHC protocol for APAF1 antibody 21710-1-APDownload protocol
IF protocol for APAF1 antibody 21710-1-APDownload protocol
IP protocol for APAF1 antibody 21710-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Theranostics

Pro-Inflammatory CXCR3 Impairs Mitochondrial Function in Experimental Non-Alcoholic Steatohepatitis.

Authors - Jinghua Du
humanWB,IHC,IF

Mol Ther Nucleic Acids

LNC473 Regulating APAF1 IRES-Dependent Translation via Competitive Sponging miR574 and miR15b: Implications in Colorectal Cancer.

Authors - Huizhe Wu
ratWB

Cell Biol Toxicol

Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca2+ homeostasis.

Authors - Yujie Zhong
human, ratWB

Mol Nutr Food Res

Diosgenin Protects Against Kidney Injury and Mitochondrial Apoptosis Induced by 3-MCPD Through the Regulation of ER Stress, Ca2+ Homeostasis, and Bcl2 Expression.

Authors - Yujie Zhong
mouseWB

Stem Cell Res Ther

Melatonin suppresses ER stress-dependent proapoptotic effects via AMPK in bone mesenchymal stem cells during mitochondrial oxidative damage.

Authors - Chongxi Fan
ratWB

Mol Neurobiol

Photobiomodulation for Global Cerebral Ischemia: Targeting Mitochondrial Dynamics and Functions.

Authors - Ruimin Wang