APOA4 Polyclonal antibody

APOA4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17996-1-AP

Synonyms

APOA4, apolipoprotein A IV



Tested Applications

Positive WB detected inhuman plasma tissue, rat liver tissue, HepG2 cells, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

17996-1-AP targets APOA4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOA4 fusion protein Ag12454
Full Name apolipoprotein A-IV
Calculated molecular weight 396 aa, 45 kDa
Observed molecular weight 45-46 kDa
GenBank accession numberBC074764
Gene symbol APOA4
Gene ID (NCBI) 337
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote ins secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.

Protocols

Product Specific Protocols
WB protocol for APOA4 antibody 17996-1-APDownload protocol
IHC protocol for APOA4 antibody 17996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cardiol Young

Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.

Authors - Li Zhang
mouseWB

J Proteomics

PMID: 31706944

Authors - Ni Yang