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APOA4 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 17996-1-AP

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

APOA4 Antibody WB human plasma tissue 17996-1-AP

human plasma tissue were subjected to SDS PAGE followed by western blot with 17996-1-AP( APOA4 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours

APOA4 Antibody IHC human liver tissue 17996-1-AP

Immunohistochemical analysis of paraffin-embedded human liver using 17996-1-AP(APOA4 antibody) at dilution of 1:100 (under 10x lens)

APOA4 Antibody IHC human liver tissue 17996-1-AP

Immunohistochemical analysis of paraffin-embedded human liver using 17996-1-AP(APOA4 antibody) at dilution of 1:100 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
human plasma tissue, rat liver tissue, HepG2 cells, mouse liver tissue
Positive IHC detected in:
human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:2000-1:16000
IHC : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
APOA4 fusion protein Ag12454
Full name:
apolipoprotein A-IV
Calculated molecular weight:
396aa,45 kDa
Observed molecular weight:
45-46 kDa
GenBank accession number:
BC074764
Gene ID (NCBI):
337
Gene symbol
APOA4
Synonyms
APOA4, apolipoprotein A IV

Background

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote insulin secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.


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