APOA4 Polyclonal antibody

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  WB analysis of human plasma using 17996-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 17996-1-AP (APOA4 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17996-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17996-1-AP (APOA4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17996-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17996-1-AP (APOA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17996-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17996-1-AP (APOA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17996-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOA4 antibody (17996-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

APOA4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 17996-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

apolipoprotein A IV, APOA 4

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Tested Applications

• Positive WB detected in: human plasma tissue, HepG2 cells, human plasma
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 2 publications below

Product Information

17996-1-AP targets APOA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APOA4 fusion protein Ag12454
• Full Name: apolipoprotein A-IV
• Calculated Molecular Weight: 396 aa, 45 kDa
• Observed Molecular Weight: 45-46 kDa
• GenBank Accession Number: BC074764
• Gene Symbol: APOA4
• Gene ID (NCBI): 337
• RRID: AB_10596638
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote ins secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.

Publications

Species	Application	Title
human	IHC	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
		JCI Insight; The distinct metabolism between large and small HDL indicates unique origins of human apolipoprotein A4; Authors - Allison B Andraski
human	WB	Int J Mol Sci; Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112).; Authors - Sotaro Motoi
mouse	WB	J Proteomics; PMID: 31706944; Authors - Ni Yang
mouse	WB	Alcohol; Hepatic COX-2 expression protects mice from an alcohol-high fat diet-induced metabolic disorder by involving protein acetylation related energy metabolism.; Authors - Minjie Chen
human	WB	Cardiol Young; Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.; Authors - Li Zhang