Tested Applications
| Positive WB detected in | human plasma tissue, HepG2 cells, human plasma |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17996-1-AP targets APOA4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12454 Product name: Recombinant human APOA4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-396 aa of BC074764 Sequence: SADQVATVMWDYFSQLSNNAKEAVEHLQKSELTQQLNALFQDKLGEVNTYAGDLQKKLVPFATELHERLAKDSEKLKEEIGKELEELRARLLPHANEVSQKIGDNLRELQQRLEPYADQLRTQVNTQAEQLRRQLTPYAQRMERVLRENADSLQASLRPHADELKAKIDQNVEELKGRLTPYADEFKVKIDQTVEELRRSLAPYAQDTQEKLNHQLEGLTFQMKKNAEELKARISASAEELRQRLAPLAEDVRGNLRGNTEGLQKSLAELGGHLDQQVEEFRRRVEPYGENFNKALVQQMEQLRQKLGPHAGDVEGHLSFLEKDLRDKVNSFFSTFKEKESQDKTLSLPELEQQQEQQQEQQQEQVQMLAPLES Predict reactive species |
| Full Name | apolipoprotein A-IV |
| Calculated Molecular Weight | 396 aa, 45 kDa |
| Observed Molecular Weight | 45-46 kDa |
| GenBank Accession Number | BC074764 |
| Gene Symbol | APOA4 |
| Gene ID (NCBI) | 337 |
| RRID | AB_10596638 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06727 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
APOA4 is a major protein component of chylomicrons in postprandial lymph and plasma. It is synthesized in the small intestine and the liver, attached to chylomicrons by enterocytes, and secreted into intestinal lymph during fat absorption. APOA4 is intimately involved in metabolism and has protective roles in cardiovascular diseases like atherosclerosis. APOA4 can promote ins secretion and maintain glucose homeostasis. It also has potent antioxidant and anti-inflammatory properties.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for APOA4 antibody 17996-1-AP | Download protocol |
| IHC protocol for APOA4 antibody 17996-1-AP | Download protocol |
| WB protocol for APOA4 antibody 17996-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The APOA4 antibody (Cat# 17996-1-AP) has 13 citations across journals including J Exp Med, JCI Insight, J Lipid Res, Int J Mol Sci, Cancers, Front Mol Biosci, J Proteomics, Alcohol, Biomol Biomed, PLoS One, Amyloid, J Clin Transl Hepatol, and Cardiol Young. Research fields span lipid metabolism, HDL/cholesterol biology, proteomics, cancer biomarkers, neurodegeneration (ALS), liver disease, and cardiovascular research.
| Species | Application | Title |
|---|---|---|
J Exp Med Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. | ||
JCI Insight The distinct metabolism between large and small HDL indicates unique origins of human apolipoprotein A4 | ||
J Clin Transl Hepatol PCSK9 and APOA4: The Dynamic Duo in TMAO-induced Cholesterol Metabolism and Cholelithiasis | ||
Int J Mol Sci Serum APOA4 Pharmacodynamically Represents Administered Recombinant Human Hepatocyte Growth Factor (E3112). | ||
J Lipid Res HDL Isolated by Immunoaffinity, Ultracentrifugation, or Precipitation is Compositionally and Functionally Distinct |
