Apolipoprotein CIII Polyclonal antibody

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  WB analysis of human plasma using 22262-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 22262-1-AP (APOC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 22262-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22262-1-AP (APOC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 22262-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22262-1-AP (APOC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Apolipoprotein CIII Polyclonal Antibody for WB, IHC, ELISA

Cat No. 22262-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

APOC3, Apo CIII, ApoC III, APOCIII, Apo-CIII

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Tested Applications

• Positive WB detected in: human plasma
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

22262-1-AP targets Apolipoprotein CIII in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: apolipoprotein C-III
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: NM_000040
• Gene Symbol: Apolipoprotein CIII
• Gene ID (NCBI): 345
• RRID: AB_3085690
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P02656
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apolipoprotein CIII (apoCIII) is the most abundant C-apolipoprotein in human and is found on very low density lipoproteins, chylomicrons, and high density lipoproteins (HDL) (PMID: 18408013). ApoCIII inhibits lipoprotein lipase as well as binding of lipoproteins to cell surface heparan sulfate proteoglycans and receptors (PMID: 26228667). ApoC-III has 3 actions that can impair plasma lipoprotein metabolism and lead to hypertriglyceridemia (PMID: 24941081).

Publications

Species	Application	Title
human	IHC	Curr Urol; Construction and evaluation of a novel prognostic risk model of aging-related genes in bladder cancer; Authors - Delin Wang