Validation Data Gallery
|Positive WB detected in||human blood tissue, A549 cells, SKOV-3 cells, HeLa cells, MCF-7 cells, HepG2 cells, A2780 cells, SW 1990 cells, human plasma tissue|
|Positive IP detected in||HepG2 cells, human plasma tissue|
|Positive IHC detected in||human kidney tissue, human spleen tissue, human liver tissue, human pancreas tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11486-2-AP targets APOL1 in WB, IP, IHC, IF, FC applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, Trypanosoma brucei|
|Host / Isotype||Rabbit / IgG|
|Immunogen||APOL1 fusion protein Ag2016|
|Full Name||apolipoprotein L, 1|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||39 kDa|
|GenBank accession number||BC017331|
|Gene ID (NCBI)||8542|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous APOL1 of 39-45 kDa in blood lysate.
|Product Specific Protocols|
|WB protocol for APOL1 antibody 11486-2-AP||Download protocol|
|IHC protocol for APOL1 antibody 11486-2-AP||Download protocol|
|IF protocol for APOL1 antibody 11486-2-AP||Download protocol|
|IP protocol for APOL1 antibody 11486-2-AP||Download protocol|
|FC protocol for APOL1 antibody 11486-2-AP||Download protocol|
|Click here to view our Standard Protocols|
Antisense oligonucleotide treatment ameliorates IFN-γ-induced proteinuria in APOL1-transgenic mice.
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All You Ever Wanted to Know About APOL1 and TLFs and Did Not Dare Ask.
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A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.
The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo.