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APOL1 Polyclonal antibody

APOL1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, FC

Conjugate

Unconjugated

Cat no : 11486-2-AP

Synonyms

APO L, APOL, APOL I, APOL1, Apolipoprotein L, Apolipoprotein L I, apolipoprotein L, 1, Apolipoprotein L1



Tested Applications

Positive WB detected inhuman blood tissue, A549 cells, SKOV-3 cells, HeLa cells, MCF-7 cells, HepG2 cells, A2780 cells, SW 1990 cells, human plasma tissue
Positive IP detected inHepG2 cells, human plasma tissue
Positive IHC detected inhuman kidney tissue, human spleen tissue, human liver tissue, human pancreas tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11486-2-AP targets APOL1 in WB, IP, IHC, IF, FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, Trypanosoma brucei
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APOL1 fusion protein Ag2016
Full Name apolipoprotein L, 1
Calculated molecular weight 44 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC017331
Gene symbol APOL1
Gene ID (NCBI) 8542
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue. This antibody recognizes the endogenous APOL1 of 39-45 kDa in blood lysate.

Protocols

Product Specific Protocols
WB protocol for APOL1 antibody 11486-2-APDownload protocol
IHC protocol for APOL1 antibody 11486-2-APDownload protocol
IF protocol for APOL1 antibody 11486-2-APDownload protocol
IP protocol for APOL1 antibody 11486-2-APDownload protocol
FC protocol for APOL1 antibody 11486-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Nat Microbiol

PMID: 30988429

Authors - Daisuke Yamane
IHC

JCI Insight

Antisense oligonucleotide treatment ameliorates IFN-γ-induced proteinuria in APOL1-transgenic mice.

Authors - Mariam Aghajan

Methods Mol Biol

All You Ever Wanted to Know About APOL1 and TLFs and Did Not Dare Ask.

Authors - Joseph Verdi
humanWB

Cells

MiR193a Modulation and Podocyte Phenotype.

Authors - Alok Jha
humanIHC

Br J Cancer

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

Authors - Xiaohui Liu
humanWB

Exp Parasitol

The effect of normal human serum on the mouse trypanosome Trypanosoma musculi in vitro and in vivo.

Authors - Xuan Zhang