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APPL1 Polyclonal antibody
APPL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 12639-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | C2C12 cells, HEK-293 cells, mouse ovary tissue, HeLa cells, mouse liver tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 6 publications below |
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
12639-1-AP targets APPL1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | APPL1 fusion protein Ag3334 |
Full Name | adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 |
Calculated Molecular Weight | 709 aa, 80 kDa |
Observed Molecular Weight | 80 kDa |
GenBank Accession Number | BC028599 |
Gene Symbol | APPL1 |
Gene ID (NCBI) | 26060 |
RRID | AB_2289669 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].
Protocols
Product Specific Protocols | |
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WB protocol for APPL1 antibody 12639-1-AP | Download protocol |
IHC protocol for APPL1 antibody 12639-1-AP | Download protocol |
IF protocol for APPL1 antibody 12639-1-AP | Download protocol |
IP protocol for APPL1 antibody 12639-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oxid Med Cell Longev DDAH1/ADMA Regulates Adiponectin Resistance in Cerebral Ischemia via the ROS/FOXO1/APR1 Pathway. | ||
J Cell Sci ESCRT-0 marks an APPL1-independent transit route for EGFR between the cell surface and the EEA1-positive early endosome. | ||
J Biol Chem Endocytosis is required for C-X-C chemokine receptor type 4 (CXCR4)-mediated Akt activation and anti-apoptotic signaling.
| ||
J Agric Food Chem Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway. | ||
Biol Reprod Myosin VI maintains the actin-dependent organization of the tubulobulbar complexes required for endocytosis during mouse spermiogenesis. | ||
Brain Res Characterization and distribution of adaptor protein containing a PH domain, PTB domain and leucine zipper motif (APPL1) in Alzheimer's disease hippocampus: an immunohistochemical study. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Ying (Verified Customer) (09-11-2023) | used for WB and IFC of brain section of wild type and APPL1 transgenic mice
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FH Sophie (Verified Customer) (11-11-2022) | I used the antibody in a western blot, my samples were primary B-cell lysates (10ug/lane). The antibody did label APPL1 around the 80kDa mark, but I did see another band lower down, around 37kDa - maybe this is a cleavage product?
|
FH X (Verified Customer) (01-18-2021) | Very strong and specific signal in WB with mouse brain lysate
|