APPL1 Polyclonal antibody

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• APPL1 Polyclonal antibody (12639-1-AP)

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APPL1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12639-1-AP Copied!

Publications (13)

Reviews (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Adapter protein containing PH domain, PTB domain and leucine zipper motif 1, APPL, DCC-interacting protein 13-alpha, Dip13 alpha, DIP13A

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  WB analysis using 12639-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12639-1-AP (APPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HEK-293 using 12639-1-AP

  WB result of APPL1 antibody (12639-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APPL1 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse ovary using 12639-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12639-1-AP (APPL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse brain using 12639-1-AP

  IP result of anti-APPL1 (IP:12639-1-AP, 4ug; Detection:12639-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human ovary tumor using 12639-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12639-1-AP (APPL1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 12639-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using APPL1 antibody (12639-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HepG2 using 12639-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human APPL1 (12639-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: C2C12 cells, HEK-293 cells, mouse ovary tissue, HeLa cells, mouse liver tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12639-1-AP targets APPL1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3334

  Product name: Recombinant human APPL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 405-709 aa of BC028599

  Sequence: QQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSSGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELNKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEGGKKRESEA

• Full Name: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
• Calculated Molecular Weight: 709 aa, 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC028599
• Gene Symbol: APPL1
• Gene ID (NCBI): 26060
• RRID: AB_2289669
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UKG1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].

Publications

What published studies show

The anti-APPL1 antibody (Cat# 12639-1-AP) has 13 total citations. Citations appear in journals including NPJ Aging, Arthritis Rheumatology, Cell Reports, Journal of Neuroscience, Journal of Biological Chemistry, Journal of Cell Science, Biochemical Pharmacology, Oxidative Medicine and Cellular Longevity, Environmental Science & Technology, Journal of Agricultural and Food Chemistry, Brain Research, Biology of Reproduction, and bioRxiv. Research fields span Alzheimer's disease and neurodegeneration, lipid metabolism, inflammatory arthritis, aging, cerebral ischemia, skeletal muscle remodeling, asthma, and endocytosis.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	NPJ Aging; Rejuvenation of leukocyte trafficking in aged mice through PEPITEM intervention; Authors - Sophie J Hopkin
human	WB	Environ Sci Technol; The Role of Human Adiponectin Receptor 1 in 2-Ethylhexyl Diphenyl Phosphate Induced Lipid Metabolic Disruption; Authors - Jiafan Feng
human	WB	Arthritis Rheumatol; PEPITEM Regulates the Synovial Microenvironment During Immune-Mediated Inflammatory Arthritis to Limit Disease.; Authors - Mussarat Wahid
mouse	WB	Cell Rep; Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease.; Authors -
rat	WB	Oxid Med Cell Longev; DDAH1/ADMA Regulates Adiponectin Resistance in Cerebral Ischemia via the ROS/FOXO1/APR1 Pathway.; Authors - Yichen Zhao
mouse	WB	J Agric Food Chem; Naringin Promotes Skeletal Muscle Fiber Remodeling by the AdipoR1-APPL1-AMPK Signaling Pathway.; Authors - Peiyuan Li

Reviews

4.6 out of 5

3 verified customer reviews

What customers say

All three reviewers rated this antibody 4–5 stars. It was validated for WB, IHC, IF, and IP across mouse brain, human fibroblasts, and primary B-cells, with dilutions ranging from 1:500 to 1:5000. Users reported strong and specific signals, with one noting an additional band around 37 kDa that may represent a cleavage product. Overall, the antibody is well-regarded for its sensitivity and specificity across multiple applications and species.

Automatically generated from the 3 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Ying (Verified Customer) (09-11-2023)

used for WB and IFC of brain section of wild type and APPL1 transgenic mice

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:500 for IFC, 1:1000 for WB
• Cell Tissue Type: mouse brain/human fibroblast

APPL1 Antibody Western Blot,Immunohistochemistry,Immunofluorescence,Immunoprecipitation validation (1:500 for IFC, 1:1000 for WB dilution) in mouse brain/human fibroblast (Cat no:12639-1-AP)

Sophie (Verified Customer) (11-11-2022)

I used the antibody in a western blot, my samples were primary B-cell lysates (10ug/lane). The antibody did label APPL1 around the 80kDa mark, but I did see another band lower down, around 37kDa - maybe this is a cleavage product?

• Applications: Western Blot
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: Primary B-cells (human, from blood)

APPL1 Antibody Western Blot validation (1:2,000 dilution) in Primary B-cells (human, from blood) (Cat no:12639-1-AP)

X (Verified Customer) (01-18-2021)

Very strong and specific signal in WB with mouse brain lysate

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mouse brain

APPL1 Antibody Western Blot validation (1:5000 dilution) in mouse brain (Cat no:12639-1-AP)