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- KD/KO Validated
ARC Monoclonal antibody
ARC Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Human, Mouse, Rat, Pig, rabbit and More (3)
WB, IHC, IF, ELISA
Cat no : 66550-1-Ig
|Positive WB detected in||Y79 cells, HEK-293 cells, Neuro-2a cells, pig brain tissue, mouse brain tissue, rat brain tissue, rabbit brain tissue, mouse cerebellum tissue, rat cerebellum tissue|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 6 publications below|
|IF||See 1 publications below|
66550-1-Ig targets ARC in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.
|Tested Reactivity||Human, Mouse, Rat, Pig, rabbit|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ARC fusion protein Ag9445|
|Full Name||activity-regulated cytoskeleton-associated protein|
|Calculated molecular weight||396 aa, 45 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC012321|
|Gene ID (NCBI)||23237|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.
|Product Specific Protocols|
|WB protocol for ARC antibody 66550-1-Ig||Download protocol|
|IHC protocol for ARC antibody 66550-1-Ig||Download protocol|
|IF protocol for ARC antibody 66550-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Front Aging Neurosci
Methyltransferase-Like 3 Rescues the Amyloid-beta protein-Induced Reduction of Activity-Regulated Cytoskeleton Associated Protein Expression via YTHDF1-Dependent N6-Methyladenosine Modification.
Phosphorylation of CREB at Serine 142 and 143 Is Essential for Visual Cortex Plasticity.
Elevation of EGR1/zif268, a Neural Activity Marker, in the Auditory Cortex of Patients with Schizophrenia and its Animal Model.
Int J Mol Sci
Transcriptomic and Proteomic Analysis of CRISPR/Cas9-Mediated ARC-Knockout HEK293 Cells.
Inhibition of NLRP1-Dependent Pyroptosis Prevents Glycogen Synthase Kinase-3β Overactivation-Induced Hyperphosphorylated Tau in Rats
Protective effect of 3-n-butylphthalide against intrastriatal injection of malonic acid-induced neurotoxicity and biochemical alteration in rats