Tested Applications
| Positive WB detected in | Y79 cells, HEK-293 cells, Neuro-2a cells, pig brain tissue, mouse brain tissue, rat brain tissue, rabbit brain tissue, mouse cerebellum tissue, rat cerebellum tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66550-1-Ig targets ARC in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9445 Product name: Recombinant human ARC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-396 aa of BC012321 Sequence: MELDHRTSGGLHAYPGPRGGQVAKPNVILQIGKCRAEMLEHVRRTHRHLLAEVSKQVERELKGLHRSVGKLESNLDGYVPTSDSQRWKKSIKACLCRCQETIANLERWVKREMHVWREVFYRLERWADRLESTGGKYPVGSESARHTVSVGVGGPESYCHEADGYDYTVSPYAITPPPAAGELPGQEPAEAQQYQPWVPGEDGQPSPGVDTQIFEDPREFLSHLEEYLRQVGGSEEYWLSQIQNHMNGPAKKWWEFKQGSVKNWVEFKKEFLQYSEGTLSREAIQRELDLPQKQGEPLDQFLWRKRDLYQTLYVDADEEEIIQYVVGTLQPKLKRFLRHPLPKTLEQLIQRGMEVQDDLEQAAEPAGPHLPVEDEAETLTPAPNSESVASDRTQPE Predict reactive species |
| Full Name | activity-regulated cytoskeleton-associated protein |
| Calculated Molecular Weight | 396 aa, 45 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC012321 |
| Gene Symbol | ARC |
| Gene ID (NCBI) | 23237 |
| RRID | AB_2881912 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q7LC44 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ARC antibody 66550-1-Ig | Download protocol |
| IHC protocol for ARC antibody 66550-1-Ig | Download protocol |
| WB protocol for ARC antibody 66550-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ARC (Activity-Regulated Cytoskeleton Associated Protein) Antibody (Cat# 66550-1-Ig) has 10 total citations published in journals including J Extracell Vesicles, Int J Mol Sci, Front Aging Neurosci, Biomed Pharmacother, Neurochem Res, Neurobiol Stress, Neurotox Res, eNeuro, and Elife. Research fields span neuroscience (synaptic plasticity, memory, LTP), neurodegeneration (amyloid-β, tau hyperphosphorylation), extracellular vesicle biology, epigenetic mRNA modification, and neuropsychiatry (schizophrenia, stress responses). Applications are predominantly WB and IF/IHC in mouse, rat, and human samples.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Glutamatergic Regulation of miRNA-Containing Intraluminal Vesicle Trafficking and Extracellular Vesicle Secretion From Cortical Neurons | ||
Int J Mol Sci Possible Role of Dopamine in the Enhancement of Intrahippocampal Arc Protein Expression Induced by Post-Learning Noradrenergic Stimulation of the Basolateral Amygdala. | ||
Int J Mol Sci Transcriptomic and Proteomic Analysis of CRISPR/Cas9-Mediated ARC-Knockout HEK293 Cells.
| ||
Front Aging Neurosci Methyltransferase-Like 3 Rescues the Amyloid-beta protein-Induced Reduction of Activity-Regulated Cytoskeleton Associated Protein Expression via YTHDF1-Dependent N6-Methyladenosine Modification. | ||
Biomed Pharmacother Protective effect of 3-n-butylphthalide against intrastriatal injection of malonic acid-induced neurotoxicity and biochemical alteration in rats | ||
Neurochem Res Elevation of EGR1/zif268, a Neural Activity Marker, in the Auditory Cortex of Patients with Schizophrenia and its Animal Model. |
Reviews
What customers say
Both reviewers used a 1:1000 dilution. One rated it 5 stars after successful use in Western Blot, IHC, and IF on human and mouse samples, simply calling it "very good." The other gave 4 stars, reporting effective detection of Arc in whole brain lysates via immunoprecipitation followed by Western Blot. Overall, users report reliable performance across multiple applications with straightforward dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Yong (Verified Customer) (10-24-2025) | Very good
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Pegah (Verified Customer) (08-24-2023) | I used this antibody 1:1000 for detection of Arc in whole brain lysates immunoprecipitated for Arc (with an anti-rabbit antibody).
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