ARC Monoclonal antibody

• Featured Product
• KD/KO Validated

ARC Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, rabbit and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1G4B5

Cat no : 66550-1-Ig

Synonyms

ARC, ARC/ARG3.1, Arg3.1, KIAA0278

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Validation Data Gallery

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  WB analysis using 66550-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66550-1-Ig (ARC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66550-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66550-1-Ig (ARC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66550-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66550-1-Ig (ARC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 66550-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using ARC antibody (66550-1-Ig, Clone: 1G4B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Y79 cells, HEK-293 cells, Neuro-2a cells, pig brain tissue, mouse brain tissue, rat brain tissue, rabbit brain tissue, mouse cerebellum tissue, rat cerebellum tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Product Information

66550-1-Ig targets ARC in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, rabbit samples.

• Tested Reactivity: Human, Mouse, Rat, Pig, rabbit
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARC fusion protein Ag9445
• Full Name: activity-regulated cytoskeleton-associated protein
• Calculated molecular weight: 396 aa, 45 kDa
• Observed molecular weight: 50 kDa
• GenBank accession number: BC012321
• Gene symbol: ARC
• Gene ID (NCBI): 23237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration. Recent study shows that expression of Arc can regulate cognitive flexibility.

Publications

Species	Application	Title
human,rat	WB,IF	Front Aging Neurosci; Methyltransferase-Like 3 Rescues the Amyloid-beta protein-Induced Reduction of Activity-Regulated Cytoskeleton Associated Protein Expression via YTHDF1-Dependent N6-Methyladenosine Modification.; Authors - Chenhaoyi Xu
mouse	WB	eNeuro; Phosphorylation of CREB at Serine 142 and 143 Is Essential for Visual Cortex Plasticity.; Authors - Nisha S Pulimood
rat	WB	Neurochem Res; Elevation of EGR1/zif268, a Neural Activity Marker, in the Auditory Cortex of Patients with Schizophrenia and its Animal Model.; Authors - Yuriko Iwakura
human	WB	Int J Mol Sci; Transcriptomic and Proteomic Analysis of CRISPR/Cas9-Mediated ARC-Knockout HEK293 Cells.; Authors - Yu-Yuan Wang ; KD Validated
rat	WB	Neurotox Res; Inhibition of NLRP1-Dependent Pyroptosis Prevents Glycogen Synthase Kinase-3β Overactivation-Induced Hyperphosphorylated Tau in Rats; Authors - Xiangying Liu
rat	WB	Biomed Pharmacother; Protective effect of 3-n-butylphthalide against intrastriatal injection of malonic acid-induced neurotoxicity and biochemical alteration in rats; Authors - Chunling Yuan