|Positive WB detected in||HeLa cells, HepG2 cells, A549 cells, Jurkat cells, mouse pancreas tissue, rat pancreas tissue|
|Positive IHC detected in||human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
23843-1-AP targets ARCN1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ARCN1 fusion protein Ag20836|
|Full Name||archain 1|
|Calculated molecular weight||511 aa, 57 kDa|
|Observed molecular weight||57 kDa, 47 kDa|
|GenBank accession number||BC093636|
|Gene ID (NCBI)||372|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Archain 1 (ARCN1), also known as delta-COP, is a subunit of the coat protein I (COPI) complex, which comprises one of the protein coats that mediate vesicle budding from the membrane. Protein sequences of ARCN1 are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. The gene of ARCN1 has been identified in chromosome band 11q23.3, and sequencing of cDNAs suggests that at least two forms of mRNA with alternative 5' ends are present. (PMID: 7782067; 20502676)
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (05-14-2021)
very good for WB detection