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  • KD/KO Validated

ARF1 Antibody

ARF1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10790-1-AP

Synonyms

ADP ribosylation factor 1, ARF1



Tested Applications

Positive WB detected inHeLa cells, COS-7 cells, HEK-293 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10790-1-AP targets ARF1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARF1 fusion protein Ag1244
Full Name ADP-ribosylation factor 1
Calculated molecular weight 21 kDa
Observed molecular weight 18-21 kDa
GenBank accession number BC009247
Gene symbol ARF1
Gene ID (NCBI) 375
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment. (PMID: 7759471, PMID: 16042562).This antibody can bind ARFs for the close sequences.

Protocols

Product Specific Protocols
WB protocol for ARF1 antibody 10790-1-APDownload protocol
IHC protocol for ARF1 antibody 10790-1-APDownload protocol
IF protocol for ARF1 antibody 10790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncotarget

ADP-ribosylation factor 1 expression regulates epithelial-mesenchymal transition and predicts poor clinical outcome in triple-negative breast cancer.

Authors - Sabrina Schlienger
  • KD Validated
humanWB

Cancer Biol Ther

The small GTPase ADP-Ribosylation Factor 1 mediates the sensitivity of triple negative breast cancer cells to EGFR tyrosine kinase inhibitors.

Authors - Eric Haines
  • KD Validated
ratWB

Cell Signal

ARF GTPases control phenotypic switching of vascular smooth muscle cells through the regulation of actin function and actin dependent gene expression.

Authors - Ricardo Charles
  • KD Validated
humanWB

Cell Signal

ARF1 controls Rac1 signaling to regulate migration of MDA-MB-231 invasive breast cancer cells.

Authors - Lewis-Saravalli Sebastian S
  • KD Validated
humanWB

Mol Biol Cell

ARF1 regulates the Rho/MLC pathway to control EGF-dependent breast cancer cell invasion.

Authors - Schlienger Sabrina S
  • KD Validated
humanWB

J Biol Chem

The adaptor proteins p66Shc and Grb2 regulate the activation of the GTPases ARF1 and ARF6 in invasive breast cancer cells.

Authors - Eric Haines

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Cristian (Verified Customer) (04-26-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa
ARF1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:10790-1-AP)
FH

Boyan (Verified Customer) (01-25-2019)

By IF, PFA fixation, this antibody gave a Actin-like staining, but in literature and also its data sheet, it should be localised to the Golgi.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: MEF
No image submitted with review