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  • KD/KO Validated

ARF1 Polyclonal antibody

ARF1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, monkey





Cat no : 10790-1-AP


ADP ribosylation factor 1, ARF1

Tested Applications

Positive WB detected inHeLa cells, rat brain tissue, mouse brain tissue, COS-7 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10790-1-AP targets ARF1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARF1 fusion protein Ag1244
Full Name ADP-ribosylation factor 1
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 18-21 kDa
GenBank Accession NumberBC009247
Gene Symbol ARF1
Gene ID (NCBI) 375
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. It mainly functions in coat recruitment. (PMID: 7759471, PMID: 16042562).This antibody can bind ARFs for the close sequences.


Product Specific Protocols
WB protocol for ARF1 antibody 10790-1-APDownload protocol
IHC protocol for ARF1 antibody 10790-1-APDownload protocol
IF protocol for ARF1 antibody 10790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Host Microbe

A Brucella Type IV Effector Targets the COG Tethering Complex to Remodel Host Secretory Traffic and Promote Intracellular Replication.

Authors - Cheryl N Miller
  • KD Validated


Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase.

Authors - Shoken Lee


Anti-allergic drug azelastine suppresses colon tumorigenesis by directly targeting ARF1 to inhibit IQGAP1-ERK-Drp1-mediated mitochondrial fission.

Authors - Hui-Fang Hu

BMC Biol

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

Authors - Xihua Yue

Fertil Steril

Pathogenic variants in TSC2 might cause premature ovarian insufficiency through activated mTOR induced hyperactivation of primordial follicles

Authors - Bingying Xu

Sci Rep

The Golgi protein ACBD3 facilitates Enterovirus 71 replication by interacting with 3A.

Authors - Xiaobo Lei
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Cristian (Verified Customer) (04-26-2019)

Immunofluorescent analysis of (PFA 3%) fixed HeLa cells using 10790-1-AP (ARF1 antibody) at dilution of 1:500 and Alexa Fluor 594-conjugated Affinipure Goat Anti-Rabbit IgG (H+L)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa
ARF1 Antibody Immunofluorescence, validation (1:500 dilution) in HeLa (Cat no:10790-1-AP)

Boyan (Verified Customer) (01-25-2019)

By IF, PFA fixation, this antibody gave a Actin-like staining, but in literature and also its data sheet, it should be localised to the Golgi.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: MEF