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ARF6-Specific Antibody 3 Publications

Rabbit Polyclonal| Catalog number: 20225-1-AP

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Con: 60 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

ARF6-Specific Antibody WB HepG2 cells 20225-1-AP

HepG2 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

ARF6-Specific Antibody IHC human liver cancer tissue 20225-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 20225-1-AP( ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ARF6-Specific Antibody IHC human liver cancer tissue 20225-1-AP

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 20225-1-AP( ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ARF6-Specific Antibody IF HeLa cells 20225-1-AP

IF result of ARF6 antibody (20225-1-AP, 1:100) with PFA fixed HeLa cells. Green: ARF6 antibody stains PM and recycling endosomes. Red: Golgi stain.

Species specificity:
human, mouse
Positive WB detected in:
HepG2 cells, A549 cells, human liver tissue, MCF-7 cells, mouse liver tissue
Positive IHC detected in:
human liver cancer tissue, human colon tissue, human heart tissue, human kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa
Recommended dilution:
WB : 1:200-1:600
IHC : 1:50-1:500
IF : 1:50-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
ADP-ribosylation factor 6
Calculated molecular weight:
20 kDa
Observed molecular weight:
20 kDa
GenBank accession number:
NM_001663
Gene ID (NCBI):
382
Gene symbol
ARF6
Synonyms
ADP ribosylation factor 6, ARF6

Background

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.


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