ARF6-Specific Polyclonal antibody

ARF6-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20225-1-AP

Synonyms

ADP ribosylation factor 6, ARF6



Tested Applications

Positive WB detected inHepG2 cells, A549 cells, human liver tissue, MCF-7 cells, mouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human colon tissue, human heart tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:600
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:100
Sample-dependent, check data in validation data gallery

Product Information

20225-1-AP targets ARF6-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ADP-ribosylation factor 6
Calculated molecular weight 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberNM_001663
Gene symbol ARF6
Gene ID (NCBI) 382
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.

Protocols

Product Specific Protocols
WB protocol for ARF6-Specific antibody 20225-1-APDownload protocol
IHC protocol for ARF6-Specific antibody 20225-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

ADP-ribosylation factor 1 expression regulates epithelial-mesenchymal transition and predicts poor clinical outcome in triple-negative breast cancer.

Authors - Sabrina Schlienger
humanIF

PLoS One

TNF Stimulates Nuclear Export and Secretion of IL-15 by Acting on CRM1 and ARF6.

Authors - Ouyang Suidong S
mouse

Sci Rep

Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.

Authors - Ermes Candiello
humanWB

Arterioscler Thromb Vasc Biol

BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.

Authors - Lin Sisi S
humanIF

Nat Cell Biol

An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.

Authors - James W Clancy