ARF6-Specific Polyclonal antibody
ARF6-Specific Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 20225-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, A549 cells, human liver tissue, MCF-7 cells, mouse liver tissue |
Positive IHC detected in | human liver cancer tissue, human colon tissue, human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:600 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
IF | See 2 publications below |
Product Information
20225-1-AP targets ARF6-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | ADP-ribosylation factor 6 |
Calculated molecular weight | 20 kDa |
Observed molecular weight | 20 kDa |
GenBank accession number | NM_001663 |
Gene symbol | ARF6 |
Gene ID (NCBI) | 382 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.
Protocols
Product Specific Protocols | |
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WB protocol for ARF6-Specific antibody 20225-1-AP | Download protocol |
IHC protocol for ARF6-Specific antibody 20225-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncotarget ADP-ribosylation factor 1 expression regulates epithelial-mesenchymal transition and predicts poor clinical outcome in triple-negative breast cancer. | ||
PLoS One TNF Stimulates Nuclear Export and Secretion of IL-15 by Acting on CRM1 and ARF6. | ||
Sci Rep Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity. | ||
Arterioscler Thromb Vasc Biol BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function. | ||