ARF6-Specific Polyclonal antibody

ARF6-Specific Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 20225-1-AP

Synonyms

ADP ribosylation factor 6, ARF6, ARF6-Specific

---

Validation Data Gallery

• 

  WB analysis using 20225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20225-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 20225-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 20225-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20225-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 20225-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20225-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20225-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20225-1-AP (ARF6-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 20225-1-AP

  IF result of ARF6 antibody (20225-1-AP, 1:100) with PFA fixed HeLa cells. Green: ARF6 antibody stains PM and recycling endosomes. Red: Golgi stain.

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, A549 cells, human liver tissue, MCF-7 cells, HEK-293 cells, rat liver tissue
• Positive IHC detected in: human liver cancer tissue, human colon tissue, human heart tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IF: See 4 publications below

Product Information

20225-1-AP targets ARF6-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ADP-ribosylation factor 6
• Calculated molecular weight: 20 kDa
• Observed molecular weight: 20 kDa
• GenBank accession number: NM_001663
• Gene symbol: ARF6
• Gene ID (NCBI): 382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20 kDa protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.

Publications

Species	Application	Title
human	WB	Oncotarget; ADP-ribosylation factor 1 expression regulates epithelial-mesenchymal transition and predicts poor clinical outcome in triple-negative breast cancer.; Authors - Sabrina Schlienger
human	WB,IF	Cell Rep; Recruitment of DNA to tumor-derived microvesicles.; Authors - James W Clancy
human	IF	PLoS One; Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT.; Authors - Masao Takeuchi
human	IF	PLoS One; TNF Stimulates Nuclear Export and Secretion of IL-15 by Acting on CRM1 and ARF6.; Authors - Ouyang Suidong S
mouse		Sci Rep; Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.; Authors - Ermes Candiello
human	WB	Arterioscler Thromb Vasc Biol; BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.; Authors - Lin Sisi S