|Positive WB detected in||HepG2 cells, A549 cells, human liver tissue, MCF-7 cells, mouse liver tissue|
|Positive IHC detected in||human liver cancer tissue, human colon tissue, human heart tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa|
|Western Blot (WB)||WB : 1:200-1:600|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:100|
|Sample-dependent, check data in validation data gallery|
20225-1-AP targets ARF6-Specific in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||ADP-ribosylation factor 6|
|Calculated molecular weight||20 kDa|
|Observed molecular weight||20 kDa|
|GenBank accession number||NM_001663|
|Gene ID (NCBI)||382|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. The ARF6 is one of best identified ARFs It is present at the plasma membrane and to some extent on endosomal membranes where it regulates the flow of trafficking into and out of the cell and the actin cytoskeleton. The antibody is specific to ARF6.
ADP-ribosylation factor 1 expression regulates epithelial-mesenchymal transition and predicts poor clinical outcome in triple-negative breast cancer.
Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT.
TNF Stimulates Nuclear Export and Secretion of IL-15 by Acting on CRM1 and ARF6.
Differential regulation of synaptic AP-2/clathrin vesicle uncoating in synaptic plasticity.
Arterioscler Thromb Vasc Biol
BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein modulates ATP-binding cassette transporter A-1 trafficking and function.
Nat Cell Biol
An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.