Arginase-1 Polyclonal antibody

Arginase-1 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 16001-1-AP

Synonyms

ARG1, Arginase 1, Arginase-1, liver Arginase, Liver type arginase



Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16001-1-AP targets Arginase-1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Arginase-1 fusion protein Ag8595
Full Name arginase, liver
Calculated molecular weight236aa,25 kDa; 322aa,35 kDa
Observed molecular weight 35-36 kDa
GenBank accession numberBC005321
Gene symbol ARG1
Gene ID (NCBI) 383
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). ARG1 mainly expresses in neurons in a normal brain. The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).

Protocols

Product Specific Protocols
WB protocol for Arginase-1 antibody 16001-1-APDownload protocol
IHC protocol for Arginase-1 antibody 16001-1-APDownload protocol
IP protocol for Arginase-1 antibody 16001-1-APDownload protocol
FC protocol for Arginase-1 antibody 16001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Biomed Res Int

Rapamycin and FTY720 Alleviate Atherosclerosis by Cross Talk of Macrophage Polarization and Autophagy.

Authors - Rui-Zhen Sun
mouseIF

Front Cell Neurosci

Targeting MAPK Pathways by Naringenin Modulates Microglia M1/M2 Polarization in Lipopolysaccharide-Stimulated Cultures.

Authors - Bei Zhang
humanIF

Nat Commun

The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.

Authors - Biao Ma
ratWB,IF

Eur J Pharmacol

Berberine attenuates arthritis in adjuvant-induced arthritic rats associated with regulating polarization of macrophages through AMPK/NF-кB pathway.

Authors - Jing Zhou
humanWB

J Proteome Res

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Authors - Yin Cao
humanWB,IHC

Cell Oncol (Dordr)

PMID: 31001733

Authors - Xiteng Yin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Debanjan (Verified Customer) (12-23-2019)

This antibody performs very well for immunohistochemistry from human skin samples

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Human skin Biopsy
Arginase-1 Antibody Immunofluorescence, validation (1:50 dilution) in Human skin Biopsy (Cat no:16001-1-AP)