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  • Western Blot (WB) analysis of various lysates using ARHGEF11 Polyclonal antibody (30508-1-AP)

    WB analysis using 30508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30508-1-AP (ARHGEF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using ARHGEF11 Polyclonal antibody (30508-1-AP)

    IHC staining of mouse colon using 30508-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30508-1-AP (ARHGEF11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARHGEF11 Polyclonal antibody

ARHGEF11 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30508-1-AP
Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Synonyms

ARHGEF11, DKFZp667F1223, GTRAP48, KIAA0380, PDZ RHOGEF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHEK-293T cells, HuH-7 cells, MDA-MB-453 cells, mouse brain tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30508-1-AP targets ARHGEF11 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGEF11 fusion protein Ag33104 Predict reactive species
Full Name Rho guanine nucleotide exchange factor (GEF) 11
Calculated Molecular Weight168 kDa
Observed Molecular Weight200-220 kDa
Gene Symbol ARHGEF11
Gene ID (NCBI) 9826
RRIDAB_3086341
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15085
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGEF11 (Rho guanine nucleotide exchange factor 11) is a specific guanine nucleotide exchange factor (GEF) for RhoA, which is a critical regulator of actin cytoskeleton dynamics and organization of dendritic spines and inhibitor of spine maintenance (PMID: 28036092). ARHGEF11 has been proven to promote tumor metastasis in glioblastoma and ovarian carcinoma (PMID: 33122451).

Protocols

Product Specific Protocols
WB protocol for ARHGEF11 antibody 30508-1-APDownload protocol
IHC protocol for ARHGEF11 antibody 30508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Udesh (Verified Customer) (08-19-2024)

Worked well at 1:2000 - uppermost bands are PDZ

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: MSCs
ARHGEF11 Antibody Western Blot validation (1:2000 dilution) in MSCs (Cat no:30508-1-AP)