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ARID3A Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 14068-1-AP

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Con: 29 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

ARID3A Antibody WB K-562 cells 14068-1-AP

K-562 cells were subjected to SDS PAGE followed by western blot with 14068-1-AP(ARID3A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours

ARID3A Antibody IHC human colon cancer tissue 14068-1-AP

Immunohistochemical of paraffin-embedded human colon cancer using 14068-1-AP(ARID3A antibody) at dilution of 1:100 (under 10x lens)

ARID3A Antibody IHC human colon cancer tissue 14068-1-AP

Immunohistochemical of paraffin-embedded human colon cancer using 14068-1-AP(ARID3A antibody) at dilution of 1:100 (under 40x lens)

ARID3A Antibody IF HepG2 cells 14068-1-AP

Immunofluorescent analysis of HepG2 cells, using ARID3A antibody 14068-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

ARID3A Antibody IP K-562 cells 14068-1-AP

IP Result of anti-ARID3A (IP:14068-1-AP, 4ug; Detection:14068-1-AP 1:1000) with K-562 cells lysate 3200ug.

Species specificity:
human, mouse, rat
Positive WB detected in:
K-562 cells
Positive IP detected in:
K-562 cells
Positive IHC detected in:
human colon cancer tissue
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ARID3A fusion protein Ag5124
Full name:
AT rich interactive domain 3A (BRIGHT-like)
Calculated molecular weight:
63 kDa
Observed molecular weight:
75 kDa
GenBank accession number:
BC060828
Gene ID (NCBI):
1820
Gene symbol
ARID3A
Synonyms
ARID3A, BRIGHT, Dead ringer like protein 1, DRIL1, DRIL3, DRX, E2F binding protein 1, E2FBP1

Background

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) [PMID:17386101]. It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but function in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling [PMID:15927959,11959810,11283269].


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