ARID3A Polyclonal antibody
ARID3A Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14068-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 3 publications below |
IP | See 1 publications below |
Product Information
14068-1-AP targets ARID3A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ARID3A fusion protein Ag5124 |
Full Name | AT rich interactive domain 3A (BRIGHT-like) |
Calculated molecular weight | 63 kDa |
Observed molecular weight | 75 kDa |
GenBank accession number | BC060828 |
Gene symbol | ARID3A |
Gene ID (NCBI) | 1820 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) [PMID:17386101]. It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but function in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling [PMID:15927959,11959810,11283269].
Protocols
Product Specific Protocols | |
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WB protocol for ARID3A antibody 14068-1-AP | Download protocol |
IHC protocol for ARID3A antibody 14068-1-AP | Download protocol |
IF protocol for ARID3A antibody 14068-1-AP | Download protocol |
IP protocol for ARID3A antibody 14068-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Anticancer Res ARID3A Positivity Correlated With Favorable Prognosis in Patients With Residual Rectal Cancer After Neoadjuvant Chemoradiotherapy. | ||
Ann Surg Oncol High Expression of AT-Rich Interactive Domain 3A (ARID3A) is Associated with Good Prognosis in Colorectal Carcinoma. | ||
Carcinogenesis ARID3A promotes the development of colorectal cancer by upregulating AURKA.
| ||
PLoS Genet Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2. |