ARID3A Polyclonal antibody

• Featured Product
• KD/KO Validated

ARID3A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14068-1-AP

Synonyms

DRIL1, Dead ringer-like protein 1, BRIGHT, B-cell regulator of IgH transcription, AT-rich interactive domain-containing protein 3A

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Validation Data Gallery

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  WB analysis using 14068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14068-1-AP (ARID3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 14068-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14068-1-AP (ARID3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14068-1-AP

  IP result of anti-ARID3A (IP:14068-1-AP, 4ug; Detection:14068-1-AP 1:1000) with K-562 cells lysate 3200ug.

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  IP experiment of K-562 using 14068-1-AP

  IP result of anti-ARID3A (IP:14068-1-AP, 4ug; Detection:14068-1-AP 1:1000) with K-562 cells lysate 3680 ug.

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  IHC staining of human colon cancer using 14068-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14068-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14068-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14068-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14068-1-AP (ARID3A antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14068-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARID3A antibody (14068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).

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  IF Staining of HepG2 using 14068-1-AP

  Immunofluorescent analysis of HepG2 cells, using ARID3A antibody 14068-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: K-562 cells, mouse placenta tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

14068-1-AP targets ARID3A in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARID3A fusion protein Ag5124
• Full Name: AT rich interactive domain 3A (BRIGHT-like)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC060828
• Gene Symbol: ARID3A
• Gene ID (NCBI): 1820
• RRID: AB_2060390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARID3A is a nuclear matrix-associated transcription factor that stimulates immunoglobulin heavy chain (IgH) expression and Cyclin E1/E2F-dependent cell cycle progression. It activates IgH transcriptional initiation by binding to ATC-rich P sites within nuclear matrix attachment regions (MARs) flanking the IgH intronic enhancer (Emu) (PMID:17386101). It is the founder of the 13-member (in humans) ARID (AT-Rich Interaction Domain) family, which share a highly conserved DNA binding domain, but functions in diverse biological processes such as cell cycle regulated events, epigenetic post-translational modification, and chromatin remodeling (PMID:15927959,11959810,11283269). The expression molecular weight observed is consistent with what has been described in the literature (PMID: 15922553, 19436740).

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
mouse	IHC	PLoS Genet; Let-7 Represses Carcinogenesis and a Stem Cell Phenotype in the Intestine via Regulation of Hmga2.; Authors - Blair B Madison
human,mouse	WB,IF	FASEB J; Exosomal let-7b-5p deriving from parietal epithelial cells attenuate renal fibrosis through suppression of TGFβR1 and ARID3a in obstructive kidney disease; Authors - Ahui Song
human	WB,IP	Carcinogenesis; ARID3A promotes the development of colorectal cancer by upregulating AURKA.; Authors - Jing Tang ; KD Validated
human	WB,IHC	Ann Surg Oncol; High Expression of AT-Rich Interactive Domain 3A (ARID3A) is Associated with Good Prognosis in Colorectal Carcinoma.; Authors - Meiying Song
human,mouse	WB,IF	Cell Biol Int; ARID3A plays a key regulatory role in palmitic acid-stimulated milk fat synthesis in mouse mammary epithelial cells; Authors - Xudong Guo ; KD Validated