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  • KD/KO Validated

BBS3 Polyclonal antibody

BBS3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12676-1-AP

Synonyms

ADP ribosylation factor like 6, ARL6, BBS3



Tested Applications

Positive WB detected inrat brain tissue, HEK-293T cells, human brain tissue, mouse brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12676-1-AP targets BBS3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BBS3 fusion protein Ag3363
Full Name ADP-ribosylation factor-like 6
Calculated molecular weight 186 aa, 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC024239
Gene symbol ARL6
Gene ID (NCBI) 84100
RRIDAB_2058675
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factor-like protein 6 (ARL6), belongs to a small GTPase superfamily, is involved in membrane protein trafficking at the base of the ciliary organelle. ARL6 is also named Bardet-Biedl syndrome 3 protein, because defects in ARL6 are a cause of Bardet-Biedl syndrome type 3 (BBS3), which is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. ARL6 can interact with many ARL6 interacting proteins and form BBSome complex with other BBS proteins including BBS1, BBS2, BBS4 and so on.

Protocols

Product Specific Protocols
WB protocol for BBS3 antibody 12676-1-APDownload protocol
IHC protocol for BBS3 antibody 12676-1-APDownload protocol
IF protocol for BBS3 antibody 12676-1-APDownload protocol
IP protocol for BBS3 antibody 12676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

EMBO J

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan
humanWB

Proc Natl Acad Sci U S A

ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.

Authors - Humbert Melissa C MC
  • KD Validated
mouseWB

PLoS Genet

BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.

Authors - Ying Hsu
humanWB

J Cell Sci

Basal body proteins regulate Notch signaling via endosomal trafficking.

Authors - Carmen C Leitch
mouseIHC,IF

Bone

Bardet-Biedl Syndrome 3 regulates development of cranial base midline structures.

Authors - Makiri Kawasaki
  • KO Validated
humanIF

Biol Open

ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.

Authors - Sayaka Fujisawa