Validation Data Gallery
|Positive WB detected in
|rat brain tissue, HEK-293T cells, human brain tissue, mouse brain tissue
|Positive IP detected in
|rat brain tissue
|Positive IHC detected in
|human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|hTERT-RPE1 cells, MDCK cells
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12676-1-AP targets BBS3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
|human, mouse, rat, canine
|Host / Isotype
|Rabbit / IgG
|BBS3 fusion protein Ag3363
|ADP-ribosylation factor-like 6
|Calculated molecular weight
|186 aa, 21 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
ADP-ribosylation factor-like protein 6 (ARL6), belongs to a small GTPase superfamily, is involved in membrane protein trafficking at the base of the ciliary organelle. ARL6 is also named Bardet-Biedl syndrome 3 protein, because defects in ARL6 are a cause of Bardet-Biedl syndrome type 3 (BBS3), which is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. ARL6 can interact with many ARL6 interacting proteins and form BBSome complex with other BBS proteins including BBS1, BBS2, BBS4 and so on.
|Product Specific Protocols
|WB protocol for BBS3 antibody 12676-1-AP
|IHC protocol for BBS3 antibody 12676-1-AP
|IF protocol for BBS3 antibody 12676-1-AP
|IP protocol for BBS3 antibody 12676-1-AP
|Click here to view our Standard Protocols
Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.
Proc Natl Acad Sci U S A
ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.
BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.
J Cell Sci
Basal body proteins regulate Notch signaling via endosomal trafficking.
Bardet-Biedl Syndrome 3 regulates development of cranial base midline structures.
ARL3 and ARL13B GTPases participate in distinct steps of INPP5E targeting to the ciliary membrane.