|Positive WB detected in||rat brain tissue, HEK-293T cells, human brain tissue, mouse brain tissue|
|Positive IP detected in||rat brain tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||hTERT-RPE1 cells, MDCK cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12676-1-AP targets BBS3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||BBS3 fusion protein Ag3363|
|Full Name||ADP-ribosylation factor-like 6|
|Calculated molecular weight||186 aa, 21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC024239|
|Gene ID (NCBI)||84100|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ADP-ribosylation factor-like protein 6 (ARL6), belongs to a small GTPase superfamily, is involved in membrane protein trafficking at the base of the ciliary organelle. ARL6 is also named Bardet-Biedl syndrome 3 protein, because defects in ARL6 are a cause of Bardet-Biedl syndrome type 3 (BBS3), which is a genetically heterogeneous disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. ARL6 can interact with many ARL6 interacting proteins and form BBSome complex with other BBS proteins including BBS1, BBS2, BBS4 and so on.
|Product Specific Protocols|
|WB protocol for BBS3 antibody 12676-1-AP||Download protocol|
|IHC protocol for BBS3 antibody 12676-1-AP||Download protocol|
|IF protocol for BBS3 antibody 12676-1-AP||Download protocol|
|IP protocol for BBS3 antibody 12676-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Absence of BBSome function leads to astrocyte reactivity in the brain.
Requirement of IFT-B-BBSome complex interaction in export of GPR161 from cilia.
Bardet-Biedl Syndrome 3 regulates development of cranial base midline structures.
J Cell Sci
Basal body proteins regulate Notch signaling via endosomal trafficking.
BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment.
Proc Natl Acad Sci U S A
ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting.