Arrestin C Polyclonal antibody

Arrestin C Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 11100-2-AP

Synonyms

ARR3, Arrestin C, ARRX, C arrestin, CAR, cArr, Cone arrestin, Retinal cone arrestin 3, X arrestin

---

Validation Data Gallery

• 

  WB analysis of human brain using 11100-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11100-2-AP (Arrestin C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11100-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11100-2-AP (Arrestin C antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 11100-2-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 11100-2-AP (Arrestin C antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse eye using 11100-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11100-2-AP (Arrestin C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 11100-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 11100-2-AP (Arrestin C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: human brain tissue, mouse eye tissue, mouse heart tissue
• Positive IHC detected in: mouse eye tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

11100-2-AP targets Arrestin C in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Arrestin C fusion protein Ag1580
• Full Name: arrestin 3, retinal (X-arrestin)
• Calculated molecular weight: 43 kDa
• Observed molecular weight: 43 kDa
• GenBank accession number: BC012096
• Gene symbol: ARR3
• Gene ID (NCBI): 407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Nat Commun; A high-risk retinoblastoma subtype with stemness features, dedifferentiated cone states and neuronal/ganglion cell gene expression.; Authors - Jing Liu
human	IHC	Cancers (Basel); Clinical, Genomic, and Pharmacological Study of MYCN-Amplified RB1 Wild-Type Metastatic Retinoblastoma.; Authors - Santiago Zugbi
human	IHC	Int J Mol Sci; Tridimensional Retinoblastoma Cultures as Vitreous Seeds Models for Live-Cell Imaging of Chemotherapy Penetration.; Authors - Ursula Winter
mouse	WB	Front Mol Neurosci; A mouse model of cone photoreceptor function loss (cpfl9) with degeneration due to a mutation in Gucy2e; Authors - Anna S E N Naggert

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Alessandro (Verified Customer) (01-19-2023)

great outcome, no unspecific staining

• Applications: Immunofluorescence