|Positive WB detected in||SH-SY5Y cells, SW480 cells, T-47D cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11274-1-AP targets ASAH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ASAH1 fusion protein Ag1799|
|Full Name||N-acylsphingosine amidohydrolase (acid ceramidase) 1|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||37-40, 50-60 kDa|
|GenBank accession number||BC016828|
|Gene ID (NCBI)||427|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). The predicted molecular weight of ASAH1 is 45 kDa. ASAH1 is a glycoprotein processed from a 55-60 kDa precursor via autoproteolytic cleavage into a mature heterodimeric enzyme formed by an α-subunit (13 kDa) and a β-subunit (37-40 kDa) (PMID: 22261821).
|Product Specific Protocols|
|WB protocol for ASAH1 antibody 11274-1-AP||Download protocol|
|IHC protocol for ASAH1 antibody 11274-1-AP||Download protocol|
|IF protocol for ASAH1 antibody 11274-1-AP||Download protocol|
|IP protocol for ASAH1 antibody 11274-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.
Aneuploid cell survival relies upon sphingolipid homeostasis.
Hum Mol Genet
Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.
Int J Mol Sci
Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation.
J Dermatol Sci
Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis.
Arch Dermatol Res
A reconstructed human epidermal keratinization culture model to characterize ceramide metabolism in the stratum corneum.