ASAH1 Polyclonal antibody

ASAH1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 11274-1-AP


AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, HSD 33, PHP, PHP32, Putative 32 kDa heart protein

Tested Applications

Positive WB detected inSH-SY5Y cells, SW480 cells, T-47D cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11274-1-AP targets ASAH1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASAH1 fusion protein Ag1799
Full Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC016828
Gene Symbol ASAH1
Gene ID (NCBI) 427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). The predicted molecular weight of ASAH1 is 45 kDa. ASAH1 is a glycoprotein processed from a 55-60 kDa precursor via autoproteolytic cleavage into a mature heterodimeric enzyme formed by an α-subunit (13 kDa) and a β-subunit (37-40 kDa) (PMID: 22261821).


Product Specific Protocols
WB protocol for ASAH1 antibody 11274-1-APDownload protocol
IHC protocol for ASAH1 antibody 11274-1-APDownload protocol
IF protocol for ASAH1 antibody 11274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.

Authors - Judith Lang

Cancer Res

Aneuploid cell survival relies upon sphingolipid homeostasis.

Authors - Yun-Chi Tang

Hum Mol Genet

Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.

Authors - Myung Jong Kim

Int J Mol Sci

Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation.

Authors - Yu-Ting Su

J Dermatol Sci

Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis.

Authors - Sawada Eri E

Arch Dermatol Res

A reconstructed human epidermal keratinization culture model to characterize ceramide metabolism in the stratum corneum.

Authors - Yoshida Naoki N