ASAH1 Polyclonal antibody

ASAH1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11274-1-AP

Synonyms

AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, HSD 33, PHP, PHP32, Putative 32 kDa heart protein



Tested Applications

Positive WB detected inrat lung tissue, human lung tissue, mouse brain tissue, mouse heart tissue, SH-SY5Y cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11274-1-AP targets ASAH1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASAH1 fusion protein Ag1799
Full Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Calculated molecular weight 44 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC016828
Gene symbol ASAH1
Gene ID (NCBI) 427
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. It is a 32 kDa heart protein. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.

Protocols

Product Specific Protocols
WB protocol for ASAH1 antibody 11274-1-APDownload protocol
IHC protocol for ASAH1 antibody 11274-1-APDownload protocol
IF protocol for ASAH1 antibody 11274-1-APDownload protocol
IP protocol for ASAH1 antibody 11274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Anat Rec (Hoboken)

PMID: 28806500

Authors - Yu-Ting Su
humanWB

Arch Dermatol Res

A reconstructed human epidermal keratinization culture model to characterize ceramide metabolism in the stratum corneum.

Authors - Yoshida Naoki N
humanWB

J Dermatol Sci

Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis.

Authors - Sawada Eri E
mouseWB

Int J Mol Sci

Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation.

Authors - Yu-Ting Su
humanWB

Hum Mol Genet

Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.

Authors - Myung Jong Kim
human,mouseWB

Cancer Res

Aneuploid cell survival relies upon sphingolipid homeostasis.

Authors - Yun-Chi Tang