ASAH1 Polyclonal antibody
ASAH1 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11274-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | SH-SY5Y cells, SW480 cells, T-47D cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | SH-SY5Y cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 9 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
11274-1-AP targets ASAH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ASAH1 fusion protein Ag1799 |
Full Name | N-acylsphingosine amidohydrolase (acid ceramidase) 1 |
Calculated molecular weight | 44 kDa |
Observed molecular weight | 37-40, 50-60 kDa |
GenBank accession number | BC016828 |
Gene symbol | ASAH1 |
Gene ID (NCBI) | 427 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). The predicted molecular weight of ASAH1 is 45 kDa. ASAH1 is a glycoprotein processed from a 55-60 kDa precursor via autoproteolytic cleavage into a mature heterodimeric enzyme formed by an α-subunit (13 kDa) and a β-subunit (37-40 kDa) (PMID: 22261821).
Protocols
Product Specific Protocols | |
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WB protocol for ASAH1 antibody 11274-1-AP | Download protocol |
IHC protocol for ASAH1 antibody 11274-1-AP | Download protocol |
IF protocol for ASAH1 antibody 11274-1-AP | Download protocol |
IP protocol for ASAH1 antibody 11274-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease. | ||
Hum Mol Genet Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function. | ||
Int J Mol Sci Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation. | ||
J Dermatol Sci Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis. | ||
Arch Dermatol Res A reconstructed human epidermal keratinization culture model to characterize ceramide metabolism in the stratum corneum. |