ASAH1 Polyclonal antibody

ASAH1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 11274-1-AP

Synonyms

AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, HSD 33, PHP, PHP32, Putative 32 kDa heart protein

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Validation Data Gallery

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  WB analysis using 11274-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11274-1-AP (ASAH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 11274-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 11274-1-AP (ASAH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11274-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11274-1-AP (ASAH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11274-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11274-1-AP (ASAH1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11274-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11274-1-AP (ASAH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11274-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11274-1-AP (ASAH1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11274-1-AP

  IP Result of anti-ASAH1 (IP:11274-1-AP, 4ug; Detection:11274-1-AP 1:300) with mouse brain tissue lysate 3200ug.

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  IHC staining of human breast cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11274-1-AP (ASAH1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11274-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11274-1-AP (ASAH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 11274-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11274-1-AP (ASAH1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, human lung tissue, mouse brain tissue, mouse heart tissue, rat lung tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11274-1-AP targets ASAH1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASAH1 fusion protein Ag1799
• Full Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1
• Calculated molecular weight: 44 kDa
• Observed molecular weight: 55-60 kDa
• GenBank accession number: BC016828
• Gene symbol: ASAH1
• Gene ID (NCBI): 427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. The predicted molecular weight of ASAH1 is 45 kDa. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme in the endosomes/lysosomes.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.; Authors - Judith Lang
human,mouse	WB	Cancer Res; Aneuploid cell survival relies upon sphingolipid homeostasis.; Authors - Yun-Chi Tang
human	WB	Hum Mol Genet; Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.; Authors - Myung Jong Kim
mouse	WB	Int J Mol Sci; Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation.; Authors - Yu-Ting Su
human	WB	J Dermatol Sci; Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis.; Authors - Sawada Eri E
human	WB	Arch Dermatol Res; A reconstructed human epidermal keratinization culture model to characterize ceramide metabolism in the stratum corneum.; Authors - Yoshida Naoki N

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