|Positive WB detected in||rat lung tissue, human lung tissue, mouse brain tissue, mouse heart tissue, SH-SY5Y cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11274-1-AP targets ASAH1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ASAH1 fusion protein Ag1799|
|Full Name||N-acylsphingosine amidohydrolase (acid ceramidase) 1|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||55-60 kDa|
|GenBank accession number||BC016828|
|Gene ID (NCBI)||427|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. It is a 32 kDa heart protein. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in Heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.
|Product Specific Protocols|
|WB protocol for ASAH1 antibody 11274-1-AP||Download protocol|
|IHC protocol for ASAH1 antibody 11274-1-AP||Download protocol|
|IF protocol for ASAH1 antibody 11274-1-AP||Download protocol|
|IP protocol for ASAH1 antibody 11274-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Anat Rec (Hoboken)
Arch Dermatol Res
A reconstructed human epidermal keratinization culture model to characterize ceramide metabolism in the stratum corneum.
J Dermatol Sci
Th1 cytokines accentuate but Th2 cytokines attenuate ceramide production in the stratum corneum of human epidermal equivalents: an implication for the disrupted barrier mechanism in atopic dermatitis.
Int J Mol Sci
Hyperbaric Oxygen Treatment Ameliorates Hearing Loss and Auditory Cortex Injury in Noise Exposed Mice by Repressing Local Ceramide Accumulation.
Hum Mol Genet
Acid ceramidase inhibition ameliorates α-synuclein accumulation upon loss of GBA1 function.
Aneuploid cell survival relies upon sphingolipid homeostasis.