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ASAH1 Monoclonal antibody
ASAH1 Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 67092-1-Ig
Validation Data Gallery
|Positive WB detected in||rat cerebellum tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
67092-1-Ig targets ASAH1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
|Tested Reactivity||Human, Mouse, Rat, Pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||ASAH1 fusion protein Ag28645|
|Full Name||N-acylsphingosine amidohydrolase (acid ceramidase) 1|
|Calculated molecular weight||44 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC016828|
|Gene ID (NCBI)||427|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hua (Verified Customer) (01-20-2023)
Good antibody. May not be able to use on certain mouse tissues.