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  • KD/KO Validated

ASAH1 Monoclonal antibody

ASAH1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, Mouse, Rat, Pig and More (1)







Cat no : 67092-1-Ig


AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, FLJ21558, FLJ22079, HSD 33, PHP, PHP32, Putative 32 kDa heart protein

Tested Applications

Positive WB detected inrat cerebellum tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67092-1-Ig targets ASAH1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Cited Reactivitymouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ASAH1 fusion protein Ag28645
Full Name N-acylsphingosine amidohydrolase (acid ceramidase) 1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC016828
Gene Symbol ASAH1
Gene ID (NCBI) 427
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.


Product Specific Protocols
WB protocol for ASAH1 antibody 67092-1-IgDownload protocol
IHC protocol for ASAH1 antibody 67092-1-IgDownload protocol
IF protocol for ASAH1 antibody 67092-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Med

Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis

Authors - Juan Xiao
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Hua (Verified Customer) (01-20-2023)

Good antibody. May not be able to use on certain mouse tissues.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:4000
  • Cell Tissue Type: Mouse brain
ASAH1 Antibody Western Blot validation (1:4000 dilution) in Mouse brain (Cat no:67092-1-Ig)