ASAH1 Monoclonal antibody

• Featured Product
• KD/KO Validated

ASAH1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1B7E4

Reviews (1)

Cat no : 67092-1-Ig

Synonyms

AC, Acid ceramidase, Acylsphingosine deacylase, ASAH, ASAH1, FLJ21558, FLJ22079, HSD 33, PHP, PHP32, Putative 32 kDa heart protein

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Validation Data Gallery

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  WB analysis of rat cerebellum using 67092-1-Ig

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67092-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67092-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig cerebellum using 67092-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67092-1-Ig (ASAH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67092-1-Ig (ASAH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67092-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67092-1-Ig (ASAH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 67092-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 67092-1-Ig (ASAH1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: rat cerebellum tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

67092-1-Ig targets ASAH1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASAH1 fusion protein Ag28645
• Full Name: N-acylsphingosine amidohydrolase (acid ceramidase) 1
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC016828
• Gene Symbol: ASAH1
• Gene ID (NCBI): 427
• RRID: AB_2882397
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASAH1, also named as AC, ACDase, Acid CDase, PHP32 and ASAH, belongs to the acid ceramidase family. ASAH1 is a lipid hydrolase that catalyzes the conversion of ceramide (cer) into sphingosine (SPH) and a free fatty acid. Mutation of ASAH1 will cause the Farber lipogranulomatosis (FL) which also known as Farber disease (FD). ASAH1 is highly expressed in heart. And it is first synthesized as an 55-60 kDa precursor and subsequently processed to the mature, heterodimeric enzyme (40 + 13 kDa) in the endosomes/lysosomes.

Publications

Species	Application	Title
mouse	WB,IF,IP	Mol Med; Acid ceramidase targeting pyruvate kinase affected trypsinogen activation in acute pancreatitis; Authors - Juan Xiao ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (01-20-2023)

Good antibody. May not be able to use on certain mouse tissues.

• Applications: Western Blot
• Primary Antibody Dilution: 1:4000
• Cell Tissue Type: Mouse brain

ASAH1 Antibody Western Blot validation (1:4000 dilution) in Mouse brain (Cat no:67092-1-Ig)