ASF1A-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

ASF1A-specific Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22259-1-AP

Synonyms

ASF1A, ASF1A-specific, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, HSPC146

---

Validation Data Gallery

• 

  WB analysis of NIH/3T3 using 22259-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22259-1-AP (ASF1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22259-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22259-1-AP (ASF1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22259-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22259-1-AP (ASF1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 22259-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22259-1-AP (ASF1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of NIH/3T3 using 22259-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 22259-1-AP (ASF1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: NIH/3T3 cells, HeLa cells
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 22259-1-AP is ASF1A-specific Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASF1A-specific fusion protein Ag17764
• Full Name: ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
• Calculated molecular weight: 204 aa, 23 kDa
• Observed molecular weight: 20-23 kDa
• GenBank accession number: BC010878
• Gene symbol: ASF1A
• Gene ID (NCBI): 25842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. This antibody is specific to the 23kd ASF1A protein.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity.; Authors - Sumin Feng ; KD Validated