ASF1A-specific Polyclonal antibody

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  WB analysis using 22259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22259-1-AP (ASF1A-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 22259-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ASF1A-specific antibody (22259-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

ASF1A-specific Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 22259-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

ASF1A, Anti-silencing function protein 1 homolog A, CGI-98, CGI 98, CCG1-interacting factor A

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

22259-1-AP targets ASF1A-specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17764

  Product name: Recombinant human ASF1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 151-204 aa of BC010878

  Sequence: INWEDNTEKLEDAESSNPNLQSLLSTDALPSASKGWSTSENSLNVMLESHMDCM

• Full Name: ASF1 anti-silencing function 1 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 204 aa, 23 kDa
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC010878
• Gene Symbol: ASF1A
• Gene ID (NCBI): 25842
• RRID: AB_2879052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y294
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. This antibody is specific to the 23kd ASF1A protein.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Nat Commun; RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity.; Authors - Sumin Feng ; KD Validated