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- KD/KO Validated
ASF1A-specific Polyclonal antibody
ASF1A-specific Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 22259-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | NIH/3T3 cells, HeLa cells |
Positive IF detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
IF | See 1 publications below |
Product Information
The immunogen of 22259-1-AP is ASF1A-specific Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ASF1A-specific fusion protein Ag17764 |
Full Name | ASF1 anti-silencing function 1 homolog A (S. cerevisiae) |
Calculated molecular weight | 204 aa, 23 kDa |
Observed molecular weight | 20-23 kDa |
GenBank accession number | BC010878 |
Gene symbol | ASF1A |
Gene ID (NCBI) | 25842 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. This antibody is specific to the 23kd ASF1A protein.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ASF1A-specific antibody 22259-1-AP | Download protocol |
IF protocol for ASF1A-specific antibody 22259-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity.
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