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ASS1 Polyclonal antibody

ASS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16210-1-AP

Synonyms

Argininosuccinate synthase, argininosuccinate synthetase 1, ASS, ASS1, Citrulline aspartate ligase, CTLN1



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse kidney tissue, rat kidney tissue, human kidney tissue, SMMC-7721 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human liver tissue, human placenta tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16210-1-AP targets ASS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ASS1 fusion protein Ag9200
Full Name argininosuccinate synthetase 1
Calculated molecular weight 412 aa, 47 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC009243
Gene symbol ASS1
Gene ID (NCBI) 445
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Protocols

Product Specific Protocols
WB protocol for ASS1 antibody 16210-1-APDownload protocol
IHC protocol for ASS1 antibody 16210-1-APDownload protocol
IF protocol for ASS1 antibody 16210-1-APDownload protocol
IP protocol for ASS1 antibody 16210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Epigenomics

Circular RNA circASS1 is down-regulated in breast cancer cells MDA-MB-231 and suppressed invasion and migration.

Authors - Jun-Chen Hou
humanWB,IHC

Acta Biochim Biophys Sin (Shanghai)

Argininosuccinate synthase 1 suppresses cancer cell invasion by inhibiting STAT3 pathway in hepatocellular carcinoma.

Authors - Xuemei Tao
humanWB

J Proteome Res

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Authors - Yin Cao
humanWB,IHC

Cell Death Dis

Androgen receptor regulates ASS1P3/miR-34a-5p/ASS1 signaling to promote renal cell carcinoma cell growth.

Authors - Kefeng Wang
humanIHC

Hepatol Commun

ASS1 Overexpression: A Hallmark of Sonic Hedgehog Hepatocellular Adenomas; Recommendations for Clinical Practice.

Authors - Margaux Sala
mouseIF

JCI Insight

Hnf4a deletion in the mouse kidney phenocopies Fanconi renotubular syndrome.

Authors - Sierra S Marable