|Positive WB detected in||HeLa cells, HEK-293 cells, mouse kidney tissue, rat kidney tissue, human kidney tissue, SMMC-7721 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human testis tissue, human liver tissue, human placenta tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16210-1-AP targets ASS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ASS1 fusion protein Ag9200|
|Full Name||argininosuccinate synthetase 1|
|Calculated molecular weight||412 aa, 47 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||BC009243|
|Gene ID (NCBI)||445|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).
|Product Specific Protocols|
|WB protocol for ASS1 antibody 16210-1-AP||Download protocol|
|IHC protocol for ASS1 antibody 16210-1-AP||Download protocol|
|IF protocol for ASS1 antibody 16210-1-AP||Download protocol|
|IP protocol for ASS1 antibody 16210-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Circular RNA circASS1 is down-regulated in breast cancer cells MDA-MB-231 and suppressed invasion and migration.
Acta Biochim Biophys Sin (Shanghai)
Argininosuccinate synthase 1 suppresses cancer cell invasion by inhibiting STAT3 pathway in hepatocellular carcinoma.
J Proteome Res
Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.
Cell Death Dis
Androgen receptor regulates ASS1P3/miR-34a-5p/ASS1 signaling to promote renal cell carcinoma cell growth.
ASS1 Overexpression: A Hallmark of Sonic Hedgehog Hepatocellular Adenomas; Recommendations for Clinical Practice.
Hnf4a deletion in the mouse kidney phenocopies Fanconi renotubular syndrome.