ASS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ASS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

32

Cat no : 16210-1-AP

Synonyms

EC:6.3.4.5, ASS

---

Validation Data Gallery

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16210-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 16210-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 16210-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16210-1-AP (ASS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16210-1-AP

  IP result of anti-ASS1 (IP:16210-1-AP, 4ug; Detection:16210-1-AP 1:1500) with HeLa cells lysate 1600ug.

• 

  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16210-1-AP (ASS1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16210-1-AP (ASS1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16210-1-AP (ASS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16210-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16210-1-AP (ASS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 16210-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ASS1 antibody (16210-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, rat kidney tissue, SMMC-7721 cells, Jurkat cells, mouse kidney tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human testis tissue, human liver tissue, human placenta tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IHC: See 13 publications below
• IF: See 6 publications below
• CoIP: See 2 publications below

Product Information

16210-1-AP targets ASS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASS1 fusion protein Ag9200
• Full Name: argininosuccinate synthetase 1
• Calculated Molecular Weight: 412 aa, 47 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC009243
• Gene Symbol: ASS1
• Gene ID (NCBI): 445
• RRID: AB_2060466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).

Publications

Species	Application	Title
mouse	IF	Immunity; Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis.; Authors - Fangzhou Lou
human	WB	Sci Transl Med; An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs.; Authors - Wei-Jian Li
human	WB	Mol Cell; Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants; Authors - Chao Yang
human	WB	Mol Cell; CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.; Authors - Ran Lin ; KO Validated
mouse	IHC	Autophagy; BNIP3-dependent mitophagy promotes cytosolic localization of LC3B and metabolic homeostasis in the liver.; Authors - Maya Z Springer
mouse	IF	J Am Soc Nephrol; Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney.; Authors - Sierra S Marable

Related Products

ASL Polyclonal antibody

16645-1-AP

OTC Polyclonal antibody

26470-1-AP

ADSS Polyclonal antibody

16373-1-AP

CPS1 Polyclonal antibody

24513-1-AP

ASNS Polyclonal antibody

14681-1-AP