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  • KD/KO Validated

ATF2-Specific Antibody

ATF2-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14834-1-AP

Synonyms

ATF2, CRE BP1, CREB 2, CREB2, CREBP1, HB16, TREB7



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14834-1-AP targets ATF2-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name activating transcription factor 2
Calculated molecular weight 55 kDa
Observed molecular weight 70 kDa
GenBank accession number BC026175
Gene symbol ATF2
Gene ID (NCBI) 1386
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human ATF2. The calculated molecular weight of ATF2 is 55 kDa, but the modified ATF2 protein is about 70 kDa (PMID: 18269766 ).

Protocols

Product Specific Protocols
WB protocol for ATF2-Specific antibody 14834-1-APDownload protocol
IHC protocol for ATF2-Specific antibody 14834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Exp Clin Cancer Res

ATF2 predicts poor prognosis and promotes malignant phenotypes in renal cell carcinoma.

Authors - Deng-Shuang Wu
mouseIF

Can J Physiol Pharmacol

SET8 participates in lipopolysaccharide-mediated BV2 cells inflammation via modulation of TICAM-2 expression.

Authors - Yanjun Zhao
ratWB

Front Endocrinol (Lausanne)

Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring.

Authors - Xiaomei Liu
humanWB

Artif Cells Nanomed Biotechnol

Molecular mechanism of miR-204 regulates proliferation, apoptosis and autophagy of cervical cancer cells by targeting ATF2.

Authors - Nan Li
  • KD Validated
humanWB

J Biol Chem

MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1.

Authors - Fanpeng Zhao
mouseWB

Cell Rep

Cold-Inducible SIRT6 Regulates Thermogenesis of Brown and Beige Fat.

Authors - Lu Yao