- Featured Product
- KD/KO Validated
ATF2-Specific Polyclonal Antibody for IHC, WB, ELISA
Cat no : 14834-1-AP
ATF2, CRE BP1, CREB 2, CREB2, CREBP1, HB16, TREB7
|Positive WB detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14834-1-AP targets ATF2-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||activating transcription factor 2|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC026175|
|Gene ID (NCBI)||1386|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ATF2, also named as CREB2 and CREBP1, contains one bZIP domain and one C2H2-type zinc finger. It belongs to the bZIP family. ATF2 binds to the cAMP-responsive element(CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. It is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. ATF2 binds DNA as a dimer and can form a homodimer in the absence of DNA. It binds through its N-terminal region to UTF1 which acts as a coactivator of ATF2 transcriptional activity. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human ATF2. The calculated molecular weight of ATF2 is 55 kDa, but the modified ATF2 protein is about 70 kDa (PMID: 18269766 ).
J Exp Clin Cancer Res
ATF2 predicts poor prognosis and promotes malignant phenotypes in renal cell carcinoma.
Can J Physiol Pharmacol
SET8 participates in lipopolysaccharide-mediated BV2 cells inflammation via modulation of TICAM-2 expression.
Front Endocrinol (Lausanne)
Maternal Protein Restriction Induces Alterations in Hepatic Unfolded Protein Response-Related Molecules in Adult Rat Offspring.
Artif Cells Nanomed Biotechnol
Molecular mechanism of miR-204 regulates proliferation, apoptosis and autophagy of cervical cancer cells by targeting ATF2.
J Biol Chem
MicroRNA-26b Inhibits Hepatitis B Virus Transcription and Replication by Targeting the Host Factor CHORDC1.
Cold-Inducible SIRT6 Regulates Thermogenesis of Brown and Beige Fat.