ATF4 Polyclonal antibody

ATF4 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 28657-1-AP

Synonyms

ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, TAXREB67, TXREB



Tested Applications

Positive WB detected inA431 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

28657-1-AP targets ATF4 in WB,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATF4 fusion protein Ag29844
Full Name activating transcription factor 4 (tax-responsive enhancer element B67)
Calculated molecular weight 39 kDa
Observed molecular weight45-50 kDa
GenBank accession numberBC022088
Gene symbol ATF4
Gene ID (NCBI) 468
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.

Protocols

Product Specific Protocols
WB protocol for ATF4 antibody 28657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Hazard Mater

Endoplasmic reticulum stress manipulates autophagic response that antagonizes polybrominated diphenyl ethers quinone induced cytotoxicity in microglial BV2 cells.

Authors - Qiushuang Zhu